BR6OX2 brassinosteroid-6-oxidase 2 [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: BR6OX2
Official Full Name: brassinosteroid-6-oxidase 2
Primary source: TAIR:AT3G30180
Locus tag: AT3G30180
See related: Araport:AT3G30180
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: brassinosteroid-6-oxidase 2; CYP85A2
Summary: Encodes a cytochrome p450 enzyme that catalyzes the last reaction in the production of brassinolide. It is capable of converting 6-deoxocastasterone into castasterone, a C-6 oxidation, as well as the further conversion of castasterone into brassinolide by a Baeyer-Villinger oxidation reaction at C-6, resulting in the formation of an unusual seven-membered lactone ring. The enzyme possesses high affinity for both C28- and C27-Brassinosteroids. The expression of the gene using a CYP85A2 promoter:LUC fusion construct was shown to be under circadian and light control.
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Genomic context

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See BR6OX2 in Genome Data Viewer

Location:: chromosome: 3

Exon count:: 9

Sequence:: Chromosome: 3; NC_003074.8 (11810726..11813883)

Chromosome 3 - NC_003074.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003074.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

CYP85A2, the cytochrome P450 enzyme that performs the last step in brassinosteroid biosynthesis (conversion of castasterone to brassinolide)(4), must be farnesylated to function in Arabidopsis. Loss of either CYP85A2 or CYP85A2 farnesylation results in reduced brassinolide accumulation and increased plant responsiveness to the hormone abscisic acid (ABA) and overall drought tolerance, explaining previous observations.
Title: Farnesylation mediates brassinosteroid biosynthesis to regulate abscisic acid responses.
The seu cyp85A2 double mutants displayed enhanced disruptions of gynoecial and ovule development.
Title: Synergistic disruptions in seuss cyp85A2 double mutants reveal a role for brassinolide synthesis during gynoecium and ovule development.
CYP85A2 catalyzes the lactonization reactions of not only castasterone but also teasterone and typhasterol
Title: Arabidopsis CYP85A2 catalyzes lactonization reactions in the biosynthesis of 2-deoxy-7-oxalactone brassinosteroids.
investigated the temporal and spatial expression patterns of CYP85A1 and CYP85A2; both promoters of CYP85A1 and CYP85A2 showed maximum activity during the first week following germination, particularly in the vascular tissues
Title: Unique and overlapping expression patterns of Arabidopsis CYP85 genes involved in brassinosteroid C-6 oxidation.
CYP85A2 is a bifunctional enzyme that possesses BR C-6 oxidase (C-6 oxidation of brassinosteroids) and brassinolide (BL) synthase activity. It is thus a cytochrome P450 that mediates Baeyer-Villiger oxidation in plants. [CYP85A2]
Title: Arabidopsis CYP85A2, a cytochrome P450, mediates the Baeyer-Villiger oxidation of castasterone to brassinolide in brassinosteroid biosynthesis.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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NM_113917.4

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables brassinolide synthase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables heme binding	IEA Inferred from Electronic Annotation more info
enables iron ion binding	IEA Inferred from Electronic Annotation more info
enables monooxygenase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within brassinosteroid biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in brassinosteroid biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in protein farnesylation	IDA Inferred from Direct Assay more info	PubMed
involved_in response to light stimulus	IEP Inferred from Expression Pattern more info	PubMed

Component	Evidence Code	Pubs
located_in endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	ISM Inferred from Sequence Model more info

General protein information

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Preferred Names: brassinosteroid-6-oxidase 2

NP_566852.1

brassinosteroid-6-oxidase 2 (BR6OX2); FUNCTIONS IN: monooxygenase activity, oxygen binding; INVOLVED IN: response to light stimulus, circadian rhythm, brassinosteroid biosynthetic process; LOCATED IN: endomembrane system; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Cytochrome P450 (InterPro:IPR001128), Cytochrome P450, E-class, group I (InterPro:IPR002401), Cytochrome P450, conserved site (InterPro:IPR017972); BEST Arabidopsis thaliana protein match is: brassinosteroid-6-oxidase 1 (TAIR:AT5G38970.1); Has 29372 Blast hits to 29308 proteins in 1622 species: Archae - 48; Bacteria - 4489; Metazoa - 10449; Fungi - 5456; Plants - 7735; Viruses - 3; Other Eukaryotes - 1192 (source: NCBI BLink).