Rbf Retinoblastoma-family protein [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Rbfprovided by FlyBase
Official Full Name: Retinoblastoma-family proteinprovided by FlyBase
Primary source: FLYBASE:FBgn0015799
Locus tag: Dmel_CG7413
See related: AllianceGenome:FB:FBgn0015799
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG7413; Dmel\CG7413; dRBF; EG:34F3.3; FBF; pRb; rb; Rb; RB; Rb1; rbf; RbF; RBF; rbf1; Rbf1; RbF1; RBf1; RBF1
Summary: Enables DNA-binding transcription factor binding activity and RNA polymerase II transcription regulatory region sequence-specific DNA binding activity. Involved in several processes, including DNA endoreduplication; negative regulation of macromolecule metabolic process; and regulation of cell cycle process. Located in chromatin and nucleus. Part of DRM complex; Myb complex; and Rb-E2F complex. Is expressed in several structures, including embryonic/larval midgut primordium; ganglia; germline cell; gut section; and head epidermis primordium. Used to study cancer and prostate cancer. Orthologous to human RBL1 (RB transcriptional corepressor like 1) and RBL2 (RB transcriptional corepressor like 2). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 1C5-1C5; 1-0 cM

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	X	NC_004354.4 (797090..801479, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	X	NC_004354.3 (691123..695512, complement)

Chromosome X - NC_004354.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Rbf/E2F1 control growth and endoreplication via steroid-independent Ecdysone Receptor signalling in Drosophila prostate-like secondary cells.
Title: Rbf/E2F1 control growth and endoreplication via steroid-independent Ecdysone Receptor signalling in Drosophila prostate-like secondary cells.
Rbf is a critical regulator of niche cells in the Drosophila testis.
Title: Retinoblastoma Intrinsically Regulates Niche Cell Quiescence, Identity, and Niche Number in the Adult Drosophila Testis.
Genetic experiments demonstrate that such metabolic changes, restricted to this unique Rbf mutant population, sensitize cells to apoptosis and define the pattern of cell death in Rbf mutant eye disc.
Title: Single cell RNA-sequencing identifies a metabolic aspect of apoptosis in Rbf mutant.
Rbf in the somatic lineage controls germline stem cell renewal and differentiation non-autonomously via essential roles in the microenvironment of the germline lineage.
Title: Rbf Regulates Drosophila Spermatogenesis via Control of Somatic Stem and Progenitor Cell Fate in the Larval Testis.
These results reveal that ESCRT-0 (ESCRT-0 components stam and hrs)mutants inhibit EGFR signaling by disrupting Rhomboid endosomal trafficking in the ligand-producing cells.
Title: ESCRT-0 complex modulates Rbf-mutant cell survival by regulating Rhomboid endosomal trafficking and EGFR signaling.
Data show that depletion of retinoblastoma factor-1 (Rbf1) is indeed associated with polarity defects in the wing and eye.
Title: Regulation of cell polarity determinants by the Retinoblastoma tumor suppressor protein.
Rbf1-induced apoptosis activates a compensatory proliferation mechanism which also depends on Slipper and TRAF1, indicating that these two proteins seem to be key players of compensatory proliferation in Drosophila.
Title: Two different specific JNK activators are required to trigger apoptosis or compensatory proliferation in response to Rbf1 in Drosophila.
These results provide a mechanism by which Drosophila Bcl-2 family proteins can control apoptosis, and shed light on a link between Rbf1 and mitochondrial dynamics in vivo.
Title: The Drosophila retinoblastoma protein, Rbf1, induces a Debcl- and Drp1-dependent mitochondrial apoptosis.
An evolutionarily conserved C-terminal "instability element" of Drosophila Rbf1 regulates cell cycle genes, and its own turnover.
Title: Role of Drosophila retinoblastoma protein instability element in cell growth and proliferation.
These results show for the first time that the caspase cleavage site of RBF could be important to control the function of RBF in tissue homeostasis in vivo.
Title: Mutating RBF can enhance its pro-apoptotic activity and uncovers a new role in tissue homeostasis.

Submit: New GeneRIF Correction See all GeneRIFs (48)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables DNA-binding transcription factor binding	IPI Inferred from Physical Interaction more info	PubMed
enables RNA polymerase II transcription regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II transcription regulatory region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II transcription regulatory region sequence-specific DNA binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables transcription cis-regulatory region binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA endoreduplication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell differentiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in chromosome organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in eggshell chorion gene amplification	TAS Traceable Author Statement more info	PubMed
involved_in endomitotic cell cycle	TAS Traceable Author Statement more info	PubMed
involved_in intrinsic apoptotic signaling pathway in response to DNA damage	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in maintenance of mitotic sister chromatid cohesion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of DNA replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of G1/S transition of mitotic cell cycle	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of G1/S transition of mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cell cycle	IEA Inferred from Electronic Annotation more info
involved_in regulation of cell population proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cell size	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of chromosome segregation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of execution phase of apoptosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info
involved_in response to starvation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of DRM complex	IDA Inferred from Direct Assay more info	PubMed
part_of Myb complex	IDA Inferred from Direct Assay more info	PubMed
part_of Rb-E2F complex	IDA Inferred from Direct Assay more info	PubMed
located_in chromatin	IBA Inferred from Biological aspect of Ancestor more info
located_in chromatin	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of transcription regulator complex	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: Retinoblastoma-family protein

Names: CG7413-PA; Rb; Rbf-PA; retinoblastoma; retinoblastoma factor; retinoblastoma factor-1; retinoblastoma family protein 1; retinoblastoma-family homolog

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_004354.4 Reference assembly

Range

797090..801479 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_080297.3 → NP_525036.2 Retinoblastoma-family protein [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_525036.2

Status: REVIEWED

UniProtKB/Swiss-Prot

O77272, Q24472, Q961U8

Related

FBpp0070141

Conserved Domains (3) summary

pfam01857
Location:582 → 704

RB_B; Retinoblastoma-associated protein B domain

pfam01858
Location:376 → 560

RB_A; Retinoblastoma-associated protein A domain

pfam11934
Location:90 → 225

DUF3452; Domain of unknown function (DUF3452)