Arhgef12 Rho guanine nucleotide exchange factor 12 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Arhgef12provided by RGD
Official Full Name: Rho guanine nucleotide exchange factor 12provided by RGD
Primary source: RGD:1305111
See related: EnsemblRapid:ENSRNOG00000008924 AllianceGenome:RGD:1305111
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Larg
Summary: Predicted to enable G protein-coupled receptor binding activity and guanyl-nucleotide exchange factor activity. Predicted to be involved in G protein-coupled receptor signaling pathway and regulation of blood pressure. Predicted to be located in membrane. Predicted to be active in cytoplasm. Orthologous to human ARHGEF12 (Rho guanine nucleotide exchange factor 12). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Lung (RPKM 673.0), Kidney (RPKM 582.1) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 8q22

Exon count:: 43

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	8	NC_086026.1 (52247047..52373158, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	8	NC_051343.1 (43350070..43475404, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	8	NC_005107.4 (47259377..47393042, complement)

Chromosome 8 - NC_086026.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

reduction of blood pressure elevation by losartan in spontaneously hypertensive rats occurs through the suppression of LARG expression and MYPT-1 phosphorylation in vascular smooth muscle cells
Title: Reduction of blood pressure elevation by losartan in spontaneously hypertensive rats through suppression of LARG expression in vascular smooth muscle cells.
Results indicate that miR-214-3p acts as a novel regulator of hypoxia-induced proliferation and migration by directly targeting ARHGEF12 and dysregulating c-jun and c-fos in PASMC.
Title: MicroRNA-214-3p Regulates Hypoxia-Mediated Pulmonary Artery Smooth Muscle Cell Proliferation and Migration by Targeting ARHGEF12.
Agonist-induced Ca2+ sensitization in smooth muscle: redundancy of Rho guanine nucleotide exchange factors (RhoGEFs) and response kinetics, a caged compound study.
Title: Agonist-induced Ca2+ sensitization in smooth muscle: redundancy of Rho guanine nucleotide exchange factors (RhoGEFs) and response kinetics, a caged compound study.
These data establish a previously uncharacterized role for the exchange factors Lsc/p115 RhoGEF and LARG in linking fibronectin signals to downstream RhoA activation.
Title: A novel role for Lsc/p115 RhoGEF and LARG in regulating RhoA activity downstream of adhesion to fibronectin.

Submit: New GeneRIF Correction

General gene information

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Markers

AW533862 (e-PCR)
- UniSTS:246016

AI102854 (e-PCR)
- UniSTS:250554

AI007655 (e-PCR)
- UniSTS:261572

RH144182 (e-PCR)
- UniSTS:233428

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables G protein-coupled receptor binding	IBA Inferred from Biological aspect of Ancestor more info
enables G protein-coupled receptor binding	ISO Inferred from Sequence Orthology more info
enables GTPase activator activity	IEA Inferred from Electronic Annotation more info
enables guanyl-nucleotide exchange factor activity	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in G protein-coupled receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within G protein-coupled receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in Rho protein signal transduction	IEA Inferred from Electronic Annotation more info
involved_in biological_process	ND No biological Data available more info
involved_in regulation of blood pressure	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: rho guanine nucleotide exchange factor 12

Names: Rho guanine nucleotide exchange factor (GEF) 12; leukemia-associated Rho guanine nucleotide exchange factor

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001013246.2 → NP_001013264.2 rho guanine nucleotide exchange factor 12

Status: VALIDATED

Source sequence(s)

JAXUCZ010000008

UniProtKB/TrEMBL

Q5BMA6

Related

ENSRNOP00000074482.2, ENSRNOT00000081007.3

Conserved Domains (4) summary

cd08754
Location:349 → 570

RGS_LARG; Regulator of G protein signaling (RGS) domain found in the leukemia-associated Rho guanine nucleotide exchange factor (RhoGEF) protein (LARG)

smart00325
Location:791 → 976

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases

cd00992
Location:71 → 145

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

cd13390
Location:995 → 1132

PH_LARG; Leukemia-associated Rho guanine nucleotide exchange factor Pleckstrin homology (PH) domain

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086026.1 Reference GRCr8

Range

52247047..52373158 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039081856.2 → XP_038937784.1 rho guanine nucleotide exchange factor 12 isoform X1

UniProtKB/TrEMBL

D3ZYR0, Q5BMA6

Related

ENSRNOP00000056613.5, ENSRNOT00000059868.6

Conserved Domains (4) summary

cd08754
Location:330 → 551

RGS_LARG; Regulator of G protein signaling (RGS) domain found in the leukemia-associated Rho guanine nucleotide exchange factor (RhoGEF) protein (LARG)

smart00325
Location:772 → 957

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases

cd00992
Location:52 → 126

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

cd13390
Location:976 → 1113

PH_LARG; Leukemia-associated Rho guanine nucleotide exchange factor Pleckstrin homology (PH) domain
XM_063265873.1 → XP_063121943.1 rho guanine nucleotide exchange factor 12 isoform X2

UniProtKB/TrEMBL

A0A8I5Y1Y6
XM_063265874.1 → XP_063121944.1 rho guanine nucleotide exchange factor 12 isoform X3

UniProtKB/TrEMBL

A6J3T5