Hes5 hes family bHLH transcription factor 5 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Hes5provided by MGI
Official Full Name: hes family bHLH transcription factor 5provided by MGI
Primary source: MGI:MGI:104876
See related: Ensembl:ENSMUSG00000048001 AllianceGenome:MGI:104876
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: bHLHb38
Summary: Enables DNA-binding transcription repressor activity, RNA polymerase II-specific. Involved in several processes, including negative regulation of cell differentiation; nephron morphogenesis; and regulation of DNA-templated transcription. Acts upstream of or within several processes, including nervous system development; regulation of nervous system development; and regulation of transcription by RNA polymerase II. Predicted to be located in nucleoplasm. Predicted to be active in nucleus. Is expressed in several structures, including central nervous system; embryo ectoderm; paraxial mesenchyme; sensory organ; and urinary system. Orthologous to human HES5 (hes family bHLH transcription factor 5). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in CNS E11.5 (RPKM 37.3), whole brain E14.5 (RPKM 16.6) and 5 other tissues See more
Orthologs: human all
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Genomic context

Location:: 4 E2; 4 86.01 cM

Exon count:: 2

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	4	NC_000070.7 (155042358..155046829)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	4	NC_000070.6 (154960626..154962372)

Chromosome 4 - NC_000070.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Notch3/Hes5 Induces Vascular Dysfunction in Hypoxia-Induced Pulmonary Hypertension Through ER Stress and Redox-Sensitive Pathways.
Title: Notch3/Hes5 Induces Vascular Dysfunction in Hypoxia-Induced Pulmonary Hypertension Through ER Stress and Redox-Sensitive Pathways.
A dynamic, spatially periodic, micro-pattern of HES5 underlies neurogenesis in the mouse spinal cord.
Title: A dynamic, spatially periodic, micro-pattern of HES5 underlies neurogenesis in the mouse spinal cord.
Repression of FBXW7 by HES5 contributes to inactivation of the TGF-beta signaling pathway and alleviation of endometriosis.
Title: Repression of FBXW7 by HES5 contributes to inactivation of the TGF-β signaling pathway and alleviation of endometriosis.
This increases the decoding capacity of HES5 oscillations and correlates with interneuron versus motor neuron cell fate.
Title: Quantitative single-cell live imaging links HES5 dynamics with cell-state and fate in murine neurogenesis.
Olig2 represses expression of the Notch signaling pathway effectors Hes1 and Hes5
Title: Olig2 and Hes regulatory dynamics during motor neuron differentiation revealed by single cell transcriptomics.
These results suggest that hyperhomocysteinemia-enhanced brain damage is associated with increased autophagy and neuronal apoptosis in Apo E(-/-) mice, in which downregulation of hes1 and hes5 is involved.
Title: Hyperhomocysteinemia-induced autophagy and apoptosis with downregulation of hairy enhancer of split 1/5 in cortical neurons in mice.
Although Hes5-GFP and Hes1 are coexpressed in particular developmental contexts, we also noted cohorts of lens or retinal cells expressing just one factor. The dynamic Hes5-GFP expression pattern, coupled with its derepressed expression in Hes1 mutants, suggests that this transgene contains the relevant cis-regulatory elements that regulate endogenous Hes5 in the mouse lens and retina.
Title: Separate and coincident expression of Hes1 and Hes5 in the developing mouse eye.
Hes5 is expressed in the nascent mesoderm of gastrulating mouse embryos. Hes5 knockdown enhances primitive erythropoiesis in mouse embryonic stem cells. A stage-specific pulse of Hes5 instructs preferential cardiac fate in mouse embryonic stem cells.
Title: Transient HES5 Activity Instructs Mesodermal Cells toward a Cardiac Fate.
Sox2 activity is crucial for the induction of the neural progenitor gene Hes5 and for subsequent differentiation of the neuronal lineage.
Title: Sox2 is required for olfactory pit formation and olfactory neurogenesis through BMP restriction and Hes5 upregulation.
Hes5 regulates the transition timing between phases for specification of neocortical neurons and between neurogenesis and gliogenesis.
Title: Hes5 regulates the transition timing of neurogenesis and gliogenesis in mammalian neocortical development.

Submit: New GeneRIF Correction See all GeneRIFs (38)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (6) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

UniSTS:484887 (e-PCR)
- UniSTS:484887

UniSTS:259615 (e-PCR)
- UniSTS:259615

Hes5 (e-PCR), detects polymorphism
- UniSTS:259615

UniSTS:235116 (e-PCR)
- UniSTS:235116

Hes5 (e-PCR), detects polymorphism
- UniSTS:547237

Hes5 (e-PCR), detects polymorphism
- UniSTS:526045

Hes5 (e-PCR), detects polymorphism
- UniSTS:526046

UniSTS:265716 (e-PCR)
- UniSTS:265716

Hes5 (e-PCR), detects polymorphism
- UniSTS:265716

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-binding transcription repressor activity, RNA polymerase II-specific	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables chromatin binding	ISO Inferred from Sequence Orthology more info
enables double-stranded DNA binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein dimerization activity	IEA Inferred from Electronic Annotation more info
enables sequence-specific double-stranded DNA binding	IEA Inferred from Electronic Annotation more info
enables sequence-specific double-stranded DNA binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of BMP signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within Notch signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in Notch signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in Notch signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in Notch signaling pathway	NAS Non-traceable Author Statement more info	PubMed
involved_in Notch signaling pathway	TAS Traceable Author Statement more info	PubMed
involved_in S-shaped body morphogenesis	IEP Inferred from Expression Pattern more info	PubMed
involved_in anterior/posterior pattern specification	IBA Inferred from Biological aspect of Ancestor more info
involved_in astrocyte differentiation	IDA Inferred from Direct Assay more info	PubMed
involved_in brain development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in camera-type eye development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cartilage development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cartilage development	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cell adhesion	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within cell maturation	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within central nervous system myelination	IMP Inferred from Mutant Phenotype more info	PubMed
NOT involved_in chondrocyte differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in comma-shaped body morphogenesis	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within establishment of epithelial cell polarity	IGI Inferred from Genetic Interaction more info	PubMed
involved_in forebrain radial glial cell differentiation	IEA Inferred from Electronic Annotation more info
involved_in forebrain radial glial cell differentiation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within glial cell fate commitment	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of inner ear auditory receptor cell differentiation	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within inner ear receptor cell stereocilium organization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in metanephric nephron tubule morphogenesis	IEP Inferred from Expression Pattern more info	PubMed
involved_in negative regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of astrocyte differentiation	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of forebrain neuron differentiation	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of forebrain neuron differentiation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of inner ear auditory receptor cell differentiation	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within negative regulation of inner ear auditory receptor cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of inner ear receptor cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of neuron differentiation	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within negative regulation of neuron differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of oligodendrocyte differentiation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within negative regulation of oligodendrocyte differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of pro-B cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of pro-B cell differentiation	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of stem cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of stem cell differentiation	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within negative regulation of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within neural tube development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of neuron differentiation	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of neuron differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neuronal stem cell population maintenance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neuronal stem cell population maintenance	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within oligodendrocyte development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of oligodendrocyte differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of BMP signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of Notch signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of cell population proliferation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell population proliferation	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of receptor signaling pathway via JAK-STAT	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of smooth muscle cell proliferation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of smooth muscle cell proliferation	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of tyrosine phosphorylation of STAT protein	IDA Inferred from Direct Assay more info	PubMed
involved_in protein-containing complex assembly	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within regulation of cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of epithelial cell proliferation	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within regulation of myelination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of neurogenesis	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within regulation of neurogenesis	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within regulation of neuron differentiation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within smoothened signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in specification of loop of Henle identity	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within telencephalon development	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in nucleoplasm	TAS Traceable Author Statement more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: transcription factor HES-5

Names: hairy and enhancer of split 5

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001370755.1 → NP_001357684.1 transcription factor HES-5 isoform 2

Status: VALIDATED

Source sequence(s)

BX004788

UniProtKB/TrEMBL

Q3U0U9

Conserved Domains (2) summary

cd00083
Location:2 → 45

HLH; Helix-loop-helix domain, found in specific DNA- binding proteins that act as transcription factors; 60-100 amino acids long. A DNA-binding basic region is followed by two alpha-helices separated by a variable loop region; HLH forms homo- and heterodimers, ...

cl02638
Location:60 → 95

Hairy_orange; Hairy Orange
NM_010419.4 → NP_034549.1 transcription factor HES-5 isoform 1

See identical proteins and their annotated locations for NP_034549.1

Status: VALIDATED

Source sequence(s)

BX004788

Consensus CDS

CCDS19018.1

UniProtKB/Swiss-Prot

P70120, Q8BVI1

UniProtKB/TrEMBL

Q3U0U9, Q499J8

Related

ENSMUSP00000051118.7, ENSMUST00000049621.7

Conserved Domains (2) summary

cd00083
Location:14 → 71

HLH; Helix-loop-helix domain, found in specific DNA- binding proteins that act as transcription factors; 60-100 amino acids long. A DNA-binding basic region is followed by two alpha-helices separated by a variable loop region; HLH forms homo- and heterodimers, ...

cl02638
Location:86 → 121

Hairy_orange; Hairy Orange

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000070.7 Reference GRCm39 C57BL/6J

Range

155042358..155046829

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006538562.5 → XP_006538625.3 transcription factor HES-5 isoform X1

Conserved Domains (2) summary

smart00511
Location:334 → 369

ORANGE; Orange domain

cd11461
Location:236 → 292

bHLH-O_HES5; basic helix-loop-helix-orange (bHLH-O) domain found in transcription factor HES-5 and similar proteins