egg eggless [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: eggprovided by FlyBase
Official Full Name: egglessprovided by FlyBase
Primary source: FLYBASE:FBgn0086908
Locus tag: Dmel_CG12196
See related: AllianceGenome:FB:FBgn0086908
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: AAF47268 Dm; AAM70794; BcDNA:AT13877; CG12196; CG30422; CG30426; dEset; Dm-setdb1; Dmel\CG12196; Dmsetdb1; DmSetdb1; DmSETDB1; dsetdb1; dSetdb1; dSetDB1; dSETDB1; Egg; EGG; Eggless; setdb1; Setdb1; SetDB1; SETDB1
Old locus tag: CG30422; CG30426; Dmel_CG30422; Dmel_CG30426
Summary: Enables histone H3K9me2 methyltransferase activity. Involved in female germ-line stem cell asymmetric division; heterochromatin organization; and transposable element silencing by piRNA-mediated heterochromatin formation. Located in cytoplasm; nucleus; and polytene chromosome. Colocalizes with chromocenter. Is expressed in several structures, including female germline cyst; ganglia; germline cell; gonad; and head epidermis primordium. Human ortholog(s) of this gene implicated in autistic disorder and lung cancer. Orthologous to several human genes including SETDB1 (SET domain bifurcated histone lysine methyltransferase 1). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 60E1-60E1; 2-107 cM

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2R	NT_033778.4 (24775534..24779901, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2R	NT_033778.3 (20663057..20667424, complement)

Chromosome 2R - NT_033778.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

H3K14ac facilitates the reinstallation of constitutive heterochromatin in Drosophila early embryos by engaging Eggless/SetDB1.
Title: H3K14ac facilitates the reinstallation of constitutive heterochromatin in Drosophila early embryos by engaging Eggless/SetDB1.
SetDB1 and Su(var)3-9 are essential for late stages of larval development of Drosophila melanogaster.
Title: SetDB1 and Su(var)3-9 are essential for late stages of larval development of Drosophila melanogaster.
SetDB1 and Su(var)3-9 play non-overlapping roles in somatic cell chromosomes of Drosophila melanogaster.
Title: SetDB1 and Su(var)3-9 play non-overlapping roles in somatic cell chromosomes of Drosophila melanogaster.
In ovarian somatic cells, Egg is monoubiquitinated and phosphorylated but only monoubiquitination is required for piRNA-mediated transposon repression. Egg monoubiquitination occurs in the nucleus. Egg has its own nuclear localization signal, and the nuclear import of Egg is Wde-independent. Wde recruits Egg to the chromatin at target gene silencing loci, but their interaction is monoubiquitin-independent.
Title: Essential roles of Windei and nuclear monoubiquitination of Eggless/SETDB1 in transposon silencing.
SETDB1 is required for chromosome binding of SU(VAR)3-9.
Title: Binding of SU(VAR)3-9 Partially Depends on SETDB1 in the Chromosomes of Drosophila melanogaster.
We show that Egg plays diverse roles in particular piRNA pathway gene expression, some long non-coding RNA expression, apoptosis-related gene regulation, and Decapentaplegic (Dpp) signaling during Drosophila oogenesis.
Title: Identification of target genes regulated by the Drosophila histone methyltransferase Eggless reveals a role of Decapentaplegic in apoptotic signaling.
a major requirement for the methyltransferase Eggless/SetDB1 in the establishment of heterochromatin
Title: Rapid embryonic cell cycles defer the establishment of heterochromatin by Eggless/SetDB1 in Drosophila.
Data show that histone-lysine N-methyltransferase (SETDB1) loss blocks germ cell differentiation.
Title: The H3K9 methyltransferase SETDB1 maintains female identity in Drosophila germ cells.
Study identified a H3K9 methyltransferase, SETDB1/eggless, a Drosophila homologue of the mammalian SETDB1, which confers the robustness for the precise determination of bristle number.
Title: Drosophila SETDB1 and caspase cooperatively fine-tune cell fate determination of sensory organ precursor.
dSETDB1 is required for the expression of a Wnt ligand, Drosophila Wingless type mouse mammary virus integration site number 4 (dWnt4) in the somatic niche.
Title: Transposon Dysregulation Modulates dWnt4 Signaling to Control Germline Stem Cell Differentiation in Drosophila.

Submit: New GeneRIF Correction See all GeneRIFs (18)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables histone H3K9 methyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables histone H3K9 methyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables histone H3K9 methyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables histone H3K9me2 methyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables histone methyltransferase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in epigenetic regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in female germ-line stem cell asymmetric division	IDA Inferred from Direct Assay more info	PubMed
involved_in heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in heterochromatin organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in heterochromatin organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of gene expression	IBA Inferred from Biological aspect of Ancestor more info
involved_in oogenesis	IDA Inferred from Direct Assay more info	PubMed
involved_in oogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transposable element silencing by heterochromatin formation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in transposable element silencing by piRNA-mediated heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
colocalizes_with chromocenter	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in polytene chromosome	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: eggless

Names: CG12196-PA; Histone-lysine N-methyltransferase; dSETDB1; egg-PA

NP_611966.3

EC 2.1.1.355

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033778.4 Reference assembly

Range

24775534..24779901 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_138122.5 → NP_611966.3 eggless [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_611966.3

Status: REVIEWED

UniProtKB/Swiss-Prot

Q29QW7, Q32KD2, Q8IHC9, Q8MMD1, Q960X1, Q9W110

Conserved Domains (3) summary

smart00317
Location:1022 → 1239

SET; SET (Su(var)3-9, Enhancer-of-zeste, Trithorax) domain

cd01395
Location:823 → 879

HMT_MBD; Methyl-CpG binding domains (MBD) present in putative histone methyltransferases (HMT) such as CLLD8 and SETDB1 proteins; CLLD8 contains a MBD, a PreSET and a bifurcated SET domain, suggesting that CLLD8 might be associated with methylation-mediated ...

pfam05033
Location:901 → 1013

Pre-SET; Pre-SET motif