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    ENDO2 endonuclease 2 [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 843158, updated on 18-Sep-2024

    Summary

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    Official Symbol
    ENDO2
    Official Full Name
    endonuclease 2
    Primary source
    TAIR:AT1G68290
    Locus tag
    AT1G68290
    See related
    Araport:AT1G68290
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    AtBFN2; endonuclease 2; T22E19.8; T22E19_8
    Summary
    Encodes an endonuclease ENDO2. ENDO2 purified from transgenic Arabidopsis digests RNA, ssDNA, and dsDNA, with a substrate preference for ssDNA and RNA. ENDO2 produced and purified from Nicotiana benthamiana expression showed no demonstrable endonuclease activity, either towards single stranded DNA or mismatches, in vitro.
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    Genomic context

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    See ENDO2 in Genome Data Viewer
    Location:
    chromosome: 1
    Exon count:
    9
    Sequence:
    Chromosome: 1; NC_003070.9 (25596671..25598438)

    Chromosome 1 - NC_003070.9Genomic Context describing neighboring genes Neighboring gene Thioesterase superfamily protein Neighboring gene pseudo Neighboring gene ncRNA Neighboring gene Adenine nucleotide alpha hydrolases-like superfamily protein Neighboring gene MIP18 family protein (DUF59) Neighboring gene myb domain protein 62

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003070.9

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Structural insights of the ssDNA binding site in the multifunctional endonuclease AtBFN2 from Arabidopsis thaliana. Yu TF, et al. PLoS One, 2014. PMID 25157844, Free PMC Article
    2. Arabidopsis ENDO2: its catalytic role and requirement of N-glycosylation for function. Ko CY, et al. J Agric Food Chem, 2012 May 23. PMID 22506810
    3. The plant s1-like nuclease family has evolved a highly diverse range of catalytic capabilities. Lesniewicz K, et al. Plant Cell Physiol, 2013 Jul. PMID 23620482
    4. Characterization of Arabidopsis thaliana mismatch specific endonucleases: application to mutation discovery by TILLING in pea. Triques K, et al. Plant J, 2007 Sep. PMID 17651368
    5. Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana. Theologis A, et al. Nature, 2000 Dec 14. PMID 11130712

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The first complete, ligand-free, AtBFN2 crystal structure, along with sulfate, phosphate and ssDNA co-crystal structures.
      Title: Structural insights of the ssDNA binding site in the multifunctional endonuclease AtBFN2 from Arabidopsis thaliana.
    2. Data indicate that five S1-like nucleases have been named as ENDO1 (BFN1, AT1G11190), ENDO2 (AT1G68290), ENDO3 (AT4G21590), ENDO4 (AT4G21585) and ENDO5 (AT4G21600).
      Title: The plant s1-like nuclease family has evolved a highly diverse range of catalytic capabilities.
    3. ENDO2 is a Ca2+/Mn2+-dependent nuclease, digesting ssDNA and RNA at neutral pH. The same enzyme is also able to digest dsDNA at acidic pH and in the simultaneous presence of Ca2+ and Zn2+ ions. The expression profile of ENDO2 gene suggests that it plays a role in the chalazal endosperm development.
      Title: The plant s1-like nuclease family has evolved a highly diverse range of catalytic capabilities.
    4. at neutral pH, endonuclease ENDO2 cleaves mismatch regions in heteroduplex DNA containing single base pair mismatches or insertion/deletion bases and can be applied to high-throughput detection of single base mutation
      Title: Arabidopsis ENDO2: its catalytic role and requirement of N-glycosylation for function.
    Submit: New GeneRIF Correction

    General gene information

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    Homology

    NM_105500.2

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables RNA endonuclease activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    NOT enables T/G mismatch-specific endonuclease activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables T/G mismatch-specific endonuclease activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables double-stranded DNA endonuclease activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables endonuclease activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables nucleic acid binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    NOT enables single-stranded DNA endodeoxyribonuclease activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables single-stranded DNA endodeoxyribonuclease activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in DNA catabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in extracellular region ISM
    Inferred from Sequence Model
    more info
    		  

    General protein information

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    Preferred Names
    endonuclease 2
    NP_176996.1
    • endonuclease 2 (ENDO 2); FUNCTIONS IN: endonuclease activity, nucleic acid binding; INVOLVED IN: DNA catabolic process; LOCATED IN: endomembrane system; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Phospholipase C/P1 nuclease, core (InterPro:IPR008947), S1/P1 nuclease (InterPro:IPR003154); BEST Arabidopsis thaliana protein match is: endonuclease 4 (TAIR:AT4G21585.1); Has 730 Blast hits to 727 proteins in 207 species: Archae - 0; Bacteria - 188; Metazoa - 0; Fungi - 100; Plants - 121; Viruses - 2; Other Eukaryotes - 319 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003070.9 Reference assembly

      Range
      25596671..25598438
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_105500.2 → NP_176996.1  endonuclease 2 [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_176996.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      Q8LCL6, Q9C9G4
      UniProtKB/TrEMBL
      A0A5S9WQP7, A0A654EM30
      Conserved Domains (1) summary
      pfam02265
      Location:28 → 290
      S1-P1_nuclease; S1/P1 Nuclease
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9C9G4.1

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