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    NAD-ME1 NAD-dependent malic enzyme 1 [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 815842, updated on 18-Sep-2024

    Summary

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    Official Symbol
    NAD-ME1
    Official Full Name
    NAD-dependent malic enzyme 1
    Primary source
    TAIR:AT2G13560
    Locus tag
    AT2G13560
    See related
    Araport:AT2G13560
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    NAD-dependent malic enzyme 1; T10F5.10; T10F5_10
    Summary
    Encodes an NAD-dependent malic enzyme (NAD-ME) that does not act on oxaloacetate, indicating that it belongs to EC 1.1.1.39. It is a member of the alpha family of NAD-MEs in plants. It appears to function as a homodimer or as a heterodimer with the beta-type NAD-ME2 (At4g00570). NAD-ME1 transcript and protein levels are higher during the night than during the day.
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    Genomic context

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    See NAD-ME1 in Genome Data Viewer
    Location:
    chromosome: 2
    Exon count:
    19
    Sequence:
    Chromosome: 2; NC_003071.7 (5649928..5655320)

    Chromosome 2 - NC_003071.7Genomic Context describing neighboring genes Neighboring gene uncharacterized protein Neighboring gene uncharacterized protein Neighboring gene uncharacterized protein Neighboring gene nuclear factor Y, subunit B7 Neighboring gene miscRNA

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003071.7

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Fine mapping identifies NAD-ME1 as a candidate underlying a major locus controlling temporal variation in primary and specialized metabolism in Arabidopsis. Francisco M, et al. Plant J, 2021 Apr. PMID 33523525
    2. Three different and tissue-specific NAD-malic enzymes generated by alternative subunit association in Arabidopsis thaliana. Tronconi MA, et al. J Biol Chem, 2010 Apr 16. PMID 20133948, Free PMC Article
    3. Arabidopsis NAD-malic enzyme functions as a homodimer and heterodimer and has a major impact on nocturnal metabolism. Tronconi MA, et al. Plant Physiol, 2008 Apr. PMID 18223148, Free PMC Article
    4. Analysis of the soluble ATP-binding proteome of plant mitochondria identifies new proteins and nucleotide triphosphate interactions within the matrix. Ito J, et al. J Proteome Res, 2006 Dec. PMID 17137349
    5. The Extra-Pathway Interactome of the TCA Cycle: Expected and Unexpected Metabolic Interactions. Zhang Y, et al. Plant Physiol, 2018 Jul. PMID 29794018, Free PMC Article

    See all (10) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Fine mapping identifies NAD-ME1 as a candidate underlying a major locus controlling temporal variation in primary and specialized metabolism in Arabidopsis.
      Title: Fine mapping identifies NAD-ME1 as a candidate underlying a major locus controlling temporal variation in primary and specialized metabolism in Arabidopsis.
    2. The characterization of recombinant NAD-ME1 and -2 indicated that both enzymes assemble as active homodimers; however, a heterodimeric enzyme (NAD-MEH) can also be detected by electrophoretic studies.
      Title: Three different and tissue-specific NAD-malic enzymes generated by alternative subunit association in Arabidopsis thaliana.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    NM_126936.4

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables ATP binding HDA PubMed 
    enables NAD binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables cobalt ion binding HDA PubMed 
    enables malate dehydrogenase (decarboxylating) (NAD+) activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    NOT enables oxaloacetate decarboxylase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein homodimerization activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein homodimerization activity IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables zinc ion binding HDA PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within malate metabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in mitochondrion HDA PubMed 
    located_in mitochondrion ISM
    Inferred from Sequence Model
    more info
    		  

    General protein information

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    Preferred Names
    NAD-dependent malic enzyme 1
    NP_178980.1
    • NAD-dependent malic enzyme 1 (NAD-ME1); FUNCTIONS IN: in 7 functions; INVOLVED IN: response to salt stress, malate metabolic process; LOCATED IN: mitochondrion, chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Malic enzyme, NAD-binding (InterPro:IPR012302), Malic oxidoreductase (InterPro:IPR001891), Malic enzyme, conserved site (InterPro:IPR015884), Malic enzyme, N-terminal (InterPro:IPR012301), NAD(P)-binding domain (InterPro:IPR016040); BEST Arabidopsis thaliana protein match is: NAD-dependent malic enzyme 2 (TAIR:AT4G00570.1); Has 9465 Blast hits to 9448 proteins in 2440 species: Archae - 143; Bacteria - 6328; Metazoa - 607; Fungi - 221; Plants - 457; Viruses - 0; Other Eukaryotes - 1709 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003071.7 Reference assembly

      Range
      5649928..5655320
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_126936.4 → NP_178980.1  NAD-dependent malic enzyme 1 [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_178980.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      Q9SIU0
      UniProtKB/TrEMBL
      A0A178VZ17, A0A5S9WY72
      Conserved Domains (1) summary
      PLN03129
      Location:25 → 619
      PLN03129; NADP-dependent malic enzyme; Provisional
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9SIU0.1

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