NIP5;1 NOD26-like intrinsic protein 5;1 [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: NIP5;1
Official Full Name: NOD26-like intrinsic protein 5;1
Primary source: TAIR:AT4G10380
Locus tag: AT4G10380
See related: Araport:AT4G10380
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: AtNIP5;1; F7L13.6; NLM6; NLM8; NOD26-like intrinsic protein 5;1; NOD26-LIKE MIP 6; NOD26-LIKE MIP 8
Summary: Boric acid channel. Essential for efficient boron uptake and plant development under boron limitation. Also functions in arsenite transport and tolerance. Localized preferentially in outer membrane domains of root cells.
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Genomic context

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See NIP5;1 in Genome Data Viewer

Location:: chromosome: 4

Exon count:: 4

Sequence:: Chromosome: 4; NC_003075.7 (6431161..6435396, complement)

Chromosome 4 - NC_003075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003075.7

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Arabidopsis transcription factor STOP1 directly activates expression of NOD26-LIKE MAJOR INTRINSIC PROTEIN5;1, and is involved in the regulation of tolerance to low-boron stress.
Title: Arabidopsis transcription factor STOP1 directly activates expression of NOD26-LIKE MAJOR INTRINSIC PROTEIN5;1, and is involved in the regulation of tolerance to low-boron stress.
Analysis of Endocytosis and Intracellular Trafficking of Boric Acid/Borate Transport Proteins in Arabidopsis.
Title: Analysis of Endocytosis and Intracellular Trafficking of Boric Acid/Borate Transport Proteins in Arabidopsis.
The results demonstrate that the polar localization of NIP5;1 is maintained by clathrin-mediated endocytosis, is dependent on phosphorylation in the TPG repeat, and is necessary for the efficient transport of boron in roots.
Title: Polar Localization of the NIP5;1 Boric Acid Channel Is Maintained by Endocytosis and Facilitates Boron Transport in Arabidopsis Roots.
The direct root uptake of urea was increased under boron deficiency, in both the high and low affinity range. This activity was entirely mediated by the NIP5;1 channel.
Title: High and Low Affinity Urea Root Uptake: Involvement of NIP5;1.
Rough mapping and whole-genome sequencing identified the mutant with altered localization of NIP5;1 as an allele of UDP-glucose 4-epimerase 4.
Title: Identification and characterization of an Arabidopsis mutant with altered localization of NIP5;1, a plasma membrane boric acid channel, reveals the requirement for D-galactose in endomembrane organization.
The 5' UTR controls boron-dependent NIP5;1 mRNA degradation and that NIP5;1 mRNA degradation is important for plant acclimation to high-boron conditions.
Title: Boron-dependent degradation of NIP5;1 mRNA for acclimation to excess boron conditions in Arabidopsis.
The residue at the H5 helix position of the aromatic/arginine filter of both OsLsi1 and AtNIP5;1 plays a key role in the permeability to silicon and boron, but there is a relatively low selectivity for arsenite.
Title: The aromatic/arginine selectivity filter of NIP aquaporins plays a critical role in substrate selectivity for silicon, boron, and arsenic.
The present study of BOR1 and NIP5;1 demonstrates the importance of selective endocytic trafficking in polar localization and degradation of plant nutrient transporters for radial transport and homeostasis of plant mineral nutrients.
Title: Polar localization and degradation of Arabidopsis boron transporters through distinct trafficking pathways.
Enhanced expression of NIP5;1 resulted in improved root elongation under boron-limiting conditions.
Title: Highly boron deficiency-tolerant plants generated by enhanced expression of NIP5;1, a boric acid channel.
Nodulin26-like Intrinsic Proteins (NIPs) AtNIP5;1 and AtNIP6;1 from A. thaliana, OsNIP2;1 and OsNIP3;2 from O. sativa, and LjNIP5;1 and LjNIP6;1 from Lotus japonicus are bi-directional As(III) channels.
Title: A subgroup of plant aquaporins facilitate the bi-directional diffusion of As(OH)3 and Sb(OH)3 across membranes.

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General gene information

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Homology

Orthologs from OrthoDB

NM_117106.3

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables active borate transmembrane transporter activity	IDA Inferred from Direct Assay more info	PubMed
enables arsenite transmembrane transporter activity	IDA Inferred from Direct Assay more info	PubMed
enables water channel activity	IDA Inferred from Direct Assay more info	PubMed
enables water channel activity	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within arsenite transport	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within borate transport	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within borate transport	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cellular response to boron-containing substance levels	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within response to arsenic-containing substance	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to boron-containing substance	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within response to boron-containing substance	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in lateral plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: NOD26-like intrinsic protein 5;1

NP_192776.1

NOD26-like intrinsic protein 5;1 (NIP5;1); CONTAINS InterPro DOMAIN/s: Major intrinsic protein, conserved site (InterPro:IPR022357), Major intrinsic protein (InterPro:IPR000425); BEST Arabidopsis thaliana protein match is: NOD26-like intrinsic protein 6;1 (TAIR:AT1G80760.1); Has 10572 Blast hits to 10477 proteins in 2169 species: Archae - 110; Bacteria - 5205; Metazoa - 1362; Fungi - 448; Plants - 2368; Viruses - 0; Other Eukaryotes - 1079 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003075.7 Reference assembly

Range

6431161..6435396 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_117106.3 → NP_192776.1 NOD26-like intrinsic protein 5;1 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_192776.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9SV84

UniProtKB/TrEMBL

A0A654FMV0, A3KPG0

Conserved Domains (1) summary

PLN00026
Location:29 → 304

PLN00026; aquaporin NIP; Provisional