U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from GEO Profiles

    • Showing Current items.

    Usp8 Ubiquitin specific protease 8 [ Drosophila melanogaster (fruit fly) ]

    Gene ID: 42509, updated on 9-Dec-2024

    Summary

    Go to the top of the page Help
    Official Symbol
    Usp8provided by FlyBase
    Official Full Name
    Ubiquitin specific protease 8provided by FlyBase
    Primary source
    FLYBASE:FBgn0038862
    Locus tag
    Dmel_CG5798
    See related
    AllianceGenome:FB:FBgn0038862
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Drosophila melanogaster
    Lineage
    Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
    Also known as
    CG5798; CT18196; Dmel\CG5798; dUBPY; Q9VDD8; ubpy; Ubpy; UbpY; UBPY; UBPY/USP8; usp8; USP8
    Summary
    Enables cysteine-type deubiquitinase activity. Involved in several processes, including negative regulation of lysosomal protein catabolic process; neuron remodeling; and positive regulation of signal transduction. Located in cytosol. Is expressed in adult head and eye. Human ortholog(s) of this gene implicated in ACTH-secreting pituitary adenoma. Orthologous to human USP8 (ubiquitin specific peptidase 8). [provided by Alliance of Genome Resources, Dec 2024]
    Orthologs
    all
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    See Usp8 in Genome Data Viewer
    Location:
    93C1-93C1; 3-71 cM
    Exon count:
    9
    Annotation release Status Assembly Chr Location
    Release 6.54 current Release 6 plus ISO1 MT (GCF_000001215.4) 3R NT_033777.3 (21134262..21138214)
    Release 5.57 previous assembly Release 5 (GCF_000001215.2) 3R NT_033777.2 (16959984..16963936)

    Chromosome 3R - NT_033777.3Genomic Context describing neighboring genes Neighboring gene tRNA methyltransferase 7-34 Neighboring gene Interacts with the C terminus of ELL 2 Neighboring gene SNF4/AMP-activated protein kinase gamma subunit Neighboring gene medial glomeruli Neighboring gene Common Dpr-interacting protein

    Genomic regions, transcripts, and products

    Go to the top of the page Help

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. CHMP1B is a target of USP8/UBPY regulated by ubiquitin during endocytosis. Crespo-Yàñez X, et al. PLoS Genet, 2018 Jun. PMID 29933386, Free PMC Article
    2. Inhibition of the deubiquitinase USP8 corrects a Drosophila PINK1 model of mitochondria dysfunction. von Stockum S, et al. Life Sci Alliance, 2019 Apr. PMID 30988163, Free PMC Article
    3. The Deubiquitinating Enzyme UBPY Is Required for Lysosomal Biogenesis and Productive Autophagy in Drosophila. Jacomin AC, et al. PLoS One, 2015. PMID 26571504, Free PMC Article
    4. USP8 Down-Regulation Promotes Parkin-Independent Mitophagy in the Drosophila Brain and in Human Neurons. Mauri S, et al. Cells, 2023 Apr 13. PMID 37190052, Free PMC Article
    5. Regulation of Smoothened Trafficking and Abundance in Hedgehog Signaling. Jia J, et al. Front Cell Dev Biol, 2022. PMID 35321245, Free PMC Article

    See all (83) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. USP8 Down-Regulation Promotes Parkin-Independent Mitophagy in the Drosophila Brain and in Human Neurons.
      Title: USP8 Down-Regulation Promotes Parkin-Independent Mitophagy in the Drosophila Brain and in Human Neurons.
    2. The deubiquitinase USP8 targets ESCRT-III to promote incomplete cell division.
      Title: The deubiquitinase USP8 targets ESCRT-III to promote incomplete cell division.
    3. Dual functions of Rack1 in regulating Hedgehog pathway.
      Title: Dual functions of Rack1 in regulating Hedgehog pathway.
    4. Ubpy loss of function results in the accumulation of autophagosomes due to a blockade of the autophagy flux.
      Title: The Deubiquitinating Enzyme UBPY Is Required for Lysosomal Biogenesis and Productive Autophagy in Drosophila.
    5. these data support a model in which Ubpy plays a dual role in both cargo deubiquitylation and the ESCRT-0 subunit Hrs stability during development.
      Title: Ubpy controls the stability of the ESCRT-0 subunit Hrs in development.
    Submit: New GeneRIF Correction

    Interactions

    Go to the top of the page Help
    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Go to the top of the page Help

    Homology

    Gene Ontology Provided by FlyBase

    Function Evidence Code Pubs
    enables cysteine-type deubiquitinase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables cysteine-type deubiquitinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables cysteine-type deubiquitinase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in Ras protein signal transduction IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in endosome organization IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in imaginal disc-derived wing margin morphogenesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in negative regulation of lysosomal protein catabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in neuron remodeling IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in positive regulation of canonical Wnt signaling pathway IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in positive regulation of receptor recycling IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in positive regulation of smoothened signaling pathway IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in protein deubiquitination IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

    Go to the top of the page Help
    Preferred Names
    ubiquitin specific protease 8
    Names
    CG5798-PA
    CG5798-PB
    UBPY ortholog
    Usp8-PA
    Usp8-PB
    NP_001247227.1
    NP_650948.2

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NT_033777.3 Reference assembly

      Range
      21134262..21138214
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_142691.2 → NP_650948.2  ubiquitin specific protease 8, isoform A [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_650948.2

      Status: REVIEWED

      UniProtKB/TrEMBL
      D0IQK1, Q9VDD8
      Related
      FBpp0083419
      Conserved Domains (4) summary
      cd02674
      Location:564 → 887
      Peptidase_C19R; A subfamily of peptidase C19. Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyze bonds involving the carboxyl ...
      pfam00443
      Location:562 → 886
      UCH; Ubiquitin carboxyl-terminal hydrolase
      pfam08969
      Location:2 → 113
      USP8_dimer; USP8 dimerization domain
      cl00125
      Location:148 → 278
      RHOD; Rhodanese Homology Domain (RHOD); an alpha beta fold domain found duplicated in the rhodanese protein. The cysteine containing enzymatically active version of the domain is also found in the Cdc25 class of protein phosphatases and a variety of proteins ...

    2. NM_001260298.1 → NP_001247227.1  ubiquitin specific protease 8, isoform B [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001247227.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      B9EQY7, H1UUC2
      Conserved Domains (2) summary
      cd02674
      Location:35 → 358
      Peptidase_C19R; A subfamily of peptidase C19. Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyze bonds involving the carboxyl ...
      pfam00443
      Location:33 → 357
      UCH; Ubiquitin carboxyl-terminal hydrolase
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    H1UUC2 H1UUC2
    Q8MQX4 Q8MQX4
    Q9VDD8 Q9VDD8

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

    Other Literature Sources
    Molecular Biology Databases
    Research Materials
    Tools
    Miscellaneous