AGO2 Argonaute family protein [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: AGO2
Official Full Name: Argonaute family protein
Primary source: TAIR:AT1G31280
Locus tag: AT1G31280
See related: Araport:AT1G31280
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: argonaute 2; AtAGO2; T19E23.7; T19E23_7
Summary: Encodes Argonaute gene that binds viral siRNAs and is involved in antiviral defense response. Regulates innate immunity.
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Genomic context

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See AGO2 in Genome Data Viewer

Location:: chromosome: 1

Exon count:: 3

Sequence:: Chromosome: 1; NC_003070.9 (11181504..11185396)

Chromosome 1 - NC_003070.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003070.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Priming for enhanced ARGONAUTE2 activation accompanies induced resistance to cucumber mosaic virus in Arabidopsis thaliana.
Title: Priming for enhanced ARGONAUTE2 activation accompanies induced resistance to cucumber mosaic virus in Arabidopsis thaliana.
Natural variation in the Arabidopsis AGO2 gene is associated with susceptibility to potato virus X.
Title: Natural variation in the Arabidopsis AGO2 gene is associated with susceptibility to potato virus X.
Antiviral ARGONAUTEs Against Turnip Crinkle Virus Revealed by Image-Based Trait Analysis.
Title: Antiviral ARGONAUTEs Against Turnip Crinkle Virus Revealed by Image-Based Trait Analysis.
MUG13.4 and AtAGO2 were both predominantly expressed in seeds and roots. Phenotypic analyses of the single and double mutants under salt stress confirmed involvement of MUG13.4-AtAGO2 complex as a component of the salt tolerance mechanism.
Title: An RNA-binding protein MUG13.4 interacts with AtAGO2 to modulate salinity tolerance in Arabidopsis.
PRMT5 directs symmetric arginine dimethylation of AGO2.The N-terminal domain of AGO2 is enriched with arginine-glycine RG/GR repeats, which are methylated by PRMT5.
Title: Dual regulation of Arabidopsis AGO2 by arginine methylation.
Data show that Argonaute family protein AGO2 and AGO3 were important for abscisic acid (ABA)-mediated resistance.
Title: Abscisic Acid Induces Resistance against Bamboo Mosaic Virus through Argonaute2 and 3.
AGO1, AGO2 and AGO10 promoted anti-TuMV defense in a modular way in various organs, with AGO2 providing a prominent antiviral role in leaves. AGO5, AGO7 and AGO10 had minor effects in leaves.
Title: Roles and programming of Arabidopsis ARGONAUTE proteins during Turnip mosaic virus infection.
Base pairing at the 15th nucleotide of a miRNA duplex is important for miRNA sorting in both Arabidopsis AGO1 and AGO2. AGO2 favours miRNA duplexes with no middle mismatches.
Title: ARGONAUTE PIWI domain and microRNA duplex structure regulate small RNA sorting in Arabidopsis.
Complementation analyses in ago mutant plants revealed that the catalytic residues of AGO1, AGO2, and AGO7 are required to restore the defects of Arabidopsis ago1-25, ago2-1, and zip-1 (AGO7-defective) mutants, respectively.
Title: Functional analysis of three Arabidopsis ARGONAUTES using slicer-defective mutants.
AGO2 and HEN1 participate in the DCL2-mediated antiviral defense to ensure the survival of Turnip crinkle virus-infected plants at high temperature.
Title: Temperature-dependent survival of Turnip crinkle virus-infected arabidopsis plants relies on an RNA silencing-based defense that requires dcl2, AGO2, and HEN1.

Submit: New GeneRIF Correction See all GeneRIFs (16)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

MASC_STS13017 (e-PCR)
- UniSTS:258592

NM_102866.3

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables mRNA binding	HDA more info	PubMed
enables mRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables siRNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within defense response to bacterium	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within defense response to virus	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within defense response to virus	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	ISM Inferred from Sequence Model more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	HDA more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Argonaute family protein

NP_174413.2

argonaute 2 (AGO2); FUNCTIONS IN: nucleic acid binding; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 12 growth stages; CONTAINS InterPro DOMAIN/s: Domain of unknown function DUF1785 (InterPro:IPR014811), Stem cell self-renewal protein Piwi (InterPro:IPR003165), Argonaute/Dicer protein, PAZ (InterPro:IPR003100), Polynucleotidyl transferase, ribonuclease H fold (InterPro:IPR012337); BEST Arabidopsis thaliana protein match is: ARGONAUTE 3 (TAIR:AT1G31290.1); Has 30240 Blast hits to 14140 proteins in 1171 species: Archae - 11; Bacteria - 7749; Metazoa - 10383; Fungi - 2602; Plants - 5076; Viruses - 357; Other Eukaryotes - 4062 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003070.9 Reference assembly

Range

11181504..11185396

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_102866.3 → NP_174413.2 Argonaute family protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_174413.2

Status: REVIEWED

UniProtKB/Swiss-Prot

Q8GX33, Q9SHF3

UniProtKB/TrEMBL

A0A178W7B1

Conserved Domains (5) summary

PLN03202
Location:137 → 957

PLN03202; protein argonaute; Provisional

cd04657
Location:528 → 963

Piwi_ago-like; Piwi_ago-like: PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of ...

pfam02170
Location:383 → 488

PAZ; PAZ domain

pfam08699
Location:317 → 366

ArgoL1; Argonaute linker 1 domain

pfam16486
Location:164 → 306

ArgoN; N-terminal domain of argonaute