RAD5 DNA/RNA helicase protein [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: RAD5
Official Full Name: DNA/RNA helicase protein
Primary source: TAIR:AT5G22750
Locus tag: AT5G22750
See related: Araport:AT5G22750
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: MDJ22.17; MDJ22_17; RAD5A
Summary: DNA repair gene. gamma-radiation hypersensitive (RAD5) involved in stable transformation and T-DNA transfer
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Genomic context

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See RAD5 in Genome Data Viewer

Location:: chromosome: 5

Exon count:: 20

Sequence:: Chromosome: 5; NC_003076.8 (7565037..7571109, complement)

Chromosome 5 - NC_003076.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003076.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

AtHRQ1 is epistatic to the ATPase RAD5A for intra- as well as interstrand crosslinking repair.
Title: The RecQ-like helicase HRQ1 is involved in DNA crosslink repair in Arabidopsis in a common pathway with the Fanconi anemia-associated nuclease FAN1 and the postreplicative repair ATPase RAD5A.
The helicase domain as well as the RING domain are essential for the function of RAD5A in DNA repair. Further de fi ning the role of RAD5A in the complex network of DNA repair factors, we present evidence that RAD5A acts independently of various other well-known DNA repair pathways.
Title: The DNA translocase RAD5A acts independently of the other main DNA repair pathways, and requires both its ATPase and RING domain for activity in Arabidopsis thaliana.
suggesting that AtRAD5a and AtREV3 are required for error-free and translesion synthesis branches of DNA-damage tolerance, respectively. [RAD5a]
Title: RAD5a and REV3 function in two alternative pathways of DNA-damage tolerance in Arabidopsis.
Ubiquitin-conjugating enzymes and RAD5a proteins are involved in the ubiquitination of plant PCNA at lysine 164.
Title: RAD5a ubiquitin ligase is involved in ubiquitination of Arabidopsis thaliana proliferating cell nuclear antigen.
RAD5A and RECQ4A act through two independent pathways for intrastrand cross-link DNA repair.
Title: RAD5A, RECQ4A, and MUS81 have specific functions in homologous recombination and define different pathways of DNA repair in Arabidopsis thaliana.
RAD5-dependent error-free branch of postreplication repair is conserved between yeast and plants, and that AtRad5a might be functionally homologous to ScRad5
Title: A homolog of ScRAD5 is involved in DNA repair and homologous recombination in Arabidopsis.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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NM_122181.3

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables ATP-dependent chromatin remodeler activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	IEA Inferred from Electronic Annotation more info
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within DNA-mediated transformation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within double-strand break repair via homologous recombination	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within double-strand break repair via homologous recombination	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within double-strand break repair via synthesis-dependent strand annealing	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within interstrand cross-link repair	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in nucleus	ISM Inferred from Sequence Model more info

General protein information

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Preferred Names: DNA/RNA helicase protein

NP_197667.1

RAD5; FUNCTIONS IN: in 6 functions; INVOLVED IN: DNA mediated transformation; EXPRESSED IN: embryo, sperm cell, seed; EXPRESSED DURING: E expanded cotyledon stage, D bilateral stage; CONTAINS InterPro DOMAIN/s: Zinc finger, RING-type, conserved site (InterPro:IPR017907), Zinc finger, RING-type (InterPro:IPR001841), Zinc finger, C3HC4 RING-type (InterPro:IPR018957), DEAD-like helicase, N-terminal (InterPro:IPR014001), DNA/RNA helicase, C-terminal (InterPro:IPR001650), Helicase, superfamily 1/2, ATP-binding domain (InterPro:IPR014021), HIP116, Rad5p N-terminal (InterPro:IPR014905), SNF2-related (InterPro:IPR000330); BEST Arabidopsis thaliana protein match is: Helicase protein with RING/U-box domain (TAIR:AT5G43530.1); Has 27107 Blast hits to 18179 proteins in 1771 species: Archae - 108; Bacteria - 7266; Metazoa - 8140; Fungi - 5089; Plants - 2446; Viruses - 173; Other Eukaryotes - 3885 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003076.8 Reference assembly

Range

7565037..7571109 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_122181.3 → NP_197667.1 DNA/RNA helicase protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_197667.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q0WQK0, Q9FNI6

UniProtKB/TrEMBL

A0A178UIW9, A0A5S9Y6Q4, A0A7G2F9F0

Conserved Domains (5) summary

COG0553
Location:186 → 1028

HepA; Superfamily II DNA or RNA helicase, SNF2 family [Transcription, Replication, recombination and repair]

COG5540
Location:765 → 837

COG5540; RING-finger-containing ubiquitin ligase [Posttranslational modification, protein turnover, chaperones]

cd18008
Location:348 → 660

DEXDc_SHPRH-like; DEXH-box helicase domain of SHPRH-like proteins

pfam08797
Location:116 → 224

HIRAN; HIRAN domain

cl21463
Location:9 → 48

UBA_like_SF; UBA domain-like superfamily