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    RIN4 RPM1 interacting protein 4 [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 822098, updated on 18-Sep-2024

    Summary

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    Official Symbol
    RIN4
    Official Full Name
    RPM1 interacting protein 4
    Primary source
    TAIR:AT3G25070
    Locus tag
    AT3G25070
    See related
    Araport:AT3G25070
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    AtRIN4; RPM1 interacting protein 4
    Summary
    Encodes a member of the R protein complex and may represent a virulence target of type III pili effector proteins (virulence factors) from bacterial pathogens, which is 'guarded' by R protein complex (RPM1 and RPS2 proteins). RIN4 physically interacts with RPS2 and RPM1 in vivo. Bacterial avirulence (Avr) effectors AvrB, AvrRpm1, and AvrRpt2 induce a mobility shift in RIN4 and expression of AvrRpt2 induces rapid degradation of RIN4. RIN4 contains 2 sites for AvrRpt2 autocleavage, called RCS1 and RCS2. Overexpression of RIN4 inhibits multiple phenotypes associated with AvrRpt2 function and also inhibits PAMP-induced defense signaling. Attached to the plasma membrane at its carboxyl terminus. Cleaved by AvrRpt2 at two PxFGxW motifs, one releasing a large portion of RIN4 from the plasma membrane and both exposing amino-terminal residues that destabilized the carboxyl-terminal cleavage products by targeting them for N-end ubiquitylation and proteasomal degradation. Major virulence target of the TTSE HopF2Pto.
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    Genomic context

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    See RIN4 in Genome Data Viewer
    Location:
    chromosome: 3
    Exon count:
    3
    Sequence:
    Chromosome: 3; NC_003074.8 (9132068..9134099)

    Chromosome 3 - NC_003074.8Genomic Context describing neighboring genes Neighboring gene xyloglucan endotransglucosylase/hydrolase 3 Neighboring gene Tetratricopeptide repeat (TPR)-like superfamily protein Neighboring gene uncharacterized protein Neighboring gene F-box associated ubiquitination effector family protein

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003074.8

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. The intrinsically disordered structural platform of the plant defence hub protein RPM1-interacting protein 4 provides insights into its mode of action in the host-pathogen interface and evolution of the nitrate-induced domain protein family. Sun X, et al. FEBS J, 2014 Sep. PMID 25039985
    2. Two Pseudomonas syringae type III effectors inhibit RIN4-regulated basal defense in Arabidopsis. Kim MG, et al. Cell, 2005 Jun 3. PMID 15935761
    3. Membrane release and destabilization of Arabidopsis RIN4 following cleavage by Pseudomonas syringae AvrRpt2. Takemoto D, et al. Mol Plant Microbe Interact, 2005 Dec. PMID 16478045
    4. The Pseudomonas syringae effector AvrRpt2 cleaves its C-terminally acylated target, RIN4, from Arabidopsis membranes to block RPM1 activation. Kim HS, et al. Proc Natl Acad Sci U S A, 2005 May 3. PMID 15845764, Free PMC Article
    5. The RIN4-like/NOI proteins NOI10 and NOI11 modulate the response to biotic stresses mediated by RIN4 in Arabidopsis. Contreras E, et al. Plant Cell Rep, 2024 Feb 15. PMID 38358510, Free PMC Article

    See all (39) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Pseudomonas effector AvrB is a glycosyltransferase that rhamnosylates plant guardee protein RIN4.
      Title: Pseudomonas effector AvrB is a glycosyltransferase that rhamnosylates plant guardee protein RIN4.
    2. The RIN4-like/NOI proteins NOI10 and NOI11 modulate the response to biotic stresses mediated by RIN4 in Arabidopsis.
      Title: The RIN4-like/NOI proteins NOI10 and NOI11 modulate the response to biotic stresses mediated by RIN4 in Arabidopsis.
    3. Direct acetylation of a conserved threonine of RIN4 by the bacterial effector HopZ5 or AvrBsT activates RPM1-dependent immunity in Arabidopsis.
      Title: Direct acetylation of a conserved threonine of RIN4 by the bacterial effector HopZ5 or AvrBsT activates RPM1-dependent immunity in Arabidopsis.
    4. The receptor-like cytoplasmic kinase CDG1 negatively regulates Arabidopsis pattern-triggered immunity and is involved in AvrRpm1-induced RIN4 phosphorylation.
      Title: The receptor-like cytoplasmic kinase CDG1 negatively regulates Arabidopsis pattern-triggered immunity and is involved in AvrRpm1-induced RIN4 phosphorylation.
    5. The resistance associated protein RIN4 promotes the extracellular transport of AtEXO70E2.
      Title: The resistance associated protein RIN4 promotes the extracellular transport of AtEXO70E2.
    6. Differential N-end Rule Degradation of RIN4/NOI Fragments Generated by the AvrRpt2 Effector Protease.
      Title: Differential N-end Rule Degradation of RIN4/NOI Fragments Generated by the AvrRpt2 Effector Protease.
    7. Bacterial effector AvrB-induced RPM1 interacting protein 4 (RIN4) phosphorylation depends on receptor-like cytoplasmic kinase (RLCK) in Arabidopsis.
      Title: The Bacterial Effector AvrB-Induced RIN4 Hyperphosphorylation Is Mediated by a Receptor-Like Cytoplasmic Kinase Complex in Arabidopsis.
    8. Transiently expressed RIN4, but not the plasma membrane (PM) protein aquaporin PIP2, recruits EXO70B1 to the plasma membrane (PM). Unlike EXO70B1, RIN4 does not recruit the core exocyst subunit SEC6 to the PM under these conditions.
      Title: RIN4 recruits the exocyst subunit EXO70B1 to the plasma membrane.
    9. RIN4 is intrinsically disordered and it's AvrRpt2 cleavage sites coincide with Molecular Recognition Features (MoRFs) that typically direct protein-protein interaction within other disordered proteins.
      Title: The intrinsically disordered structural platform of the plant defence hub protein RPM1-interacting protein 4 provides insights into its mode of action in the host-pathogen interface and evolution of the nitrate-induced domain protein family.
    10. RIN4 T21D/S160D/T166D phosphomimetic mutants exhibited enhanced disease susceptibility upon surface inoculation with P. syringae, wider stomatal apertures, and enhanced plasma membrane H(+)-ATPase activity.
      Title: Phosphorylation of the Plant Immune Regulator RPM1-INTERACTING PROTEIN4 Enhances Plant Plasma Membrane H⁺-ATPase Activity and Inhibits Flagellin-Triggered Immune Responses in Arabidopsis.
    Submit: New GeneRIF Correction See all GeneRIFs (24)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    NM_001338741.1
    NM_113411.3

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within defense response to bacterium IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within immune response IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within negative regulation of plant-type hypersensitive response IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in negative regulation of plant-type hypersensitive response IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within plant-type hypersensitive response IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within protein phosphorylation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within response to molecule of bacterial origin IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytosol HDA PubMed 
    located_in extrinsic component of plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus ISM
    Inferred from Sequence Model
    more info
    		  
    located_in plasma membrane HDA PubMed 
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane TAS
    Traceable Author Statement
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    RPM1 interacting protein 4
    NP_189143.2
    • RPM1 interacting protein 4 (RIN4); FUNCTIONS IN: protein binding; INVOLVED IN: in 6 processes; LOCATED IN: plasma membrane, membrane; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: RPM1-interacting protein 4, defence response (InterPro:IPR008700); BEST Arabidopsis thaliana protein match is: defense protein-related (TAIR:AT5G48657.2); Has 373 Blast hits to 371 proteins in 56 species: Archae - 0; Bacteria - 8; Metazoa - 13; Fungi - 2; Plants - 331; Viruses - 0; Other Eukaryotes - 19 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003074.8 Reference assembly

      Range
      9132068..9134099
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_113411.3 → NP_189143.2  RPM1 interacting protein 4 [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_189143.2

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      Q8GYN5, Q9LSG9
      UniProtKB/TrEMBL
      A0A178VFN6, A0A654FAI0, B2BDQ1, Q2HII6
      Conserved Domains (1) summary
      pfam05627
      Location:143 → 178
      AvrRpt-cleavage; Cleavage site for pathogenic type III effector avirulence factor Avr

    2. NM_001338741.1 → NP_001325873.1  RPM1 interacting protein 4 [Arabidopsis thaliana]

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A178VDD7, A0A1I9LMJ4
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q8GYN5.1

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