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    NAD-ME2 NAD-dependent malic enzyme 2 [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 828222, updated on 18-Sep-2024

    Summary

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    Official Symbol
    NAD-ME2
    Official Full Name
    NAD-dependent malic enzyme 2
    Primary source
    TAIR:AT4G00570
    Locus tag
    AT4G00570
    See related
    Araport:AT4G00570
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    F6N23.16; F6N23_16; NAD-dependent malic enzyme 2
    Summary
    Encodes an NAD-dependent malic enzyme (NAD-ME) that does not act on oxaloacetate, indicating that it belongs to EC 1.1.1.39. It is a member of the beta family of NAD-MEs in plants. It appears to function as a homodimer or as a heterodimer with the alpha-type NAD-ME2 (At2g13560). NAD-ME2 transcript and protein levels are higher during the night than during the day.
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    Genomic context

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    See NAD-ME2 in Genome Data Viewer
    Location:
    chromosome: 4
    Exon count:
    19
    Sequence:
    Chromosome: 4; NC_003075.7 (242461..246804, complement)

    Chromosome 4 - NC_003075.7Genomic Context describing neighboring genes Neighboring gene myb domain protein 3r2 Neighboring gene digalactosyl diacylglycerol deficient 2 Neighboring gene NAD(P)-binding Rossmann-fold superfamily protein Neighboring gene ncRNA Neighboring gene COP1-interacting protein-like protein Neighboring gene uncharacterized protein

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003075.7

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. NAD-malic enzymes of Arabidopsis thaliana display distinct kinetic mechanisms that support differences in physiological control. Tronconi MA, et al. Biochem J, 2010 Sep 1. PMID 20528775
    2. Three different and tissue-specific NAD-malic enzymes generated by alternative subunit association in Arabidopsis thaliana. Tronconi MA, et al. J Biol Chem, 2010 Apr 16. PMID 20133948, Free PMC Article
    3. Arabidopsis NAD-malic enzyme functions as a homodimer and heterodimer and has a major impact on nocturnal metabolism. Tronconi MA, et al. Plant Physiol, 2008 Apr. PMID 18223148, Free PMC Article
    4. Analysis of the soluble ATP-binding proteome of plant mitochondria identifies new proteins and nucleotide triphosphate interactions within the matrix. Ito J, et al. J Proteome Res, 2006 Dec. PMID 17137349
    5. Divalent metal ions in plant mitochondria and their role in interactions with proteins and oxidative stress-induced damage to respiratory function. Tan YF, et al. Plant Physiol, 2010 Feb. PMID 20018591, Free PMC Article

    See all (10) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The first 176 amino acids are associated with the differences observed in the kinetic mechanisms of NAD-ME1 and NAD-ME2.
      Title: NAD-malic enzymes of Arabidopsis thaliana display distinct kinetic mechanisms that support differences in physiological control.
    2. The characterization of recombinant NAD-ME1 and -2 indicated that both enzymes assemble as active homodimers; however, a heterodimeric enzyme (NAD-MEH) can also be detected by electrophoretic studies.
      Title: Three different and tissue-specific NAD-malic enzymes generated by alternative subunit association in Arabidopsis thaliana.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    NM_116281.4

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables ATP binding HDA PubMed 
    enables NAD binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables cobalt ion binding HDA PubMed 
    enables malate dehydrogenase (decarboxylating) (NAD+) activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    NOT enables oxaloacetate decarboxylase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein homodimerization activity IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables zinc ion binding HDA PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within malate metabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytosol HDA PubMed 
    located_in mitochondrion HDA PubMed 
    located_in mitochondrion ISM
    Inferred from Sequence Model
    more info
    		  

    General protein information

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    Preferred Names
    NAD-dependent malic enzyme 2
    NP_191966.2
    • NAD-dependent malic enzyme 2 (NAD-ME2); FUNCTIONS IN: in 7 functions; INVOLVED IN: malate metabolic process; LOCATED IN: mitochondrion, chloroplast; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Malic enzyme, NAD-binding (InterPro:IPR012302), Malic oxidoreductase (InterPro:IPR001891), Malic enzyme, conserved site (InterPro:IPR015884), Malic enzyme, N-terminal (InterPro:IPR012301), NAD(P)-binding domain (InterPro:IPR016040); BEST Arabidopsis thaliana protein match is: NAD-dependent malic enzyme 1 (TAIR:AT2G13560.1); Has 9343 Blast hits to 9326 proteins in 2439 species: Archae - 143; Bacteria - 6323; Metazoa - 607; Fungi - 223; Plants - 463; Viruses - 0; Other Eukaryotes - 1584 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003075.7 Reference assembly

      Range
      242461..246804 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_116281.4 → NP_191966.2  NAD-dependent malic enzyme 2 [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_191966.2

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      O65266, Q8L7K9, Q9M162
      UniProtKB/TrEMBL
      A0A5S9XNW8, A0A654FKW0
      Conserved Domains (1) summary
      PLN03129
      Location:17 → 604
      PLN03129; NADP-dependent malic enzyme; Provisional
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q8L7K9.1

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