DWF4 Cytochrome P450 superfamily protein [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: DWF4
Official Full Name: Cytochrome P450 superfamily protein
Primary source: TAIR:AT3G50660
Locus tag: AT3G50660
See related: Araport:AT3G50660
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: AtDWF4; CLM; CLOMAZONE-RESISTANT; CYP90B1; CYTOCHROME P450 90B1; DWARF 4; PARTIALLY SUPPRESSING COI1 INSENSITIVITY TO JA 1; PARTIALLY SUPPRESSING COI1 INSENSITIVITY TO JA 1; PSC1; SAV1; SHADE AVOIDANCE 1; SNP2; STEROID 22-ALPHA-HYDROXYLASE; SUPPRESSOR OF NPH4 2
Summary: Encodes a 22alpha hydroxylase whose reaction is a rate-limiting step in brassinosteroid biosynthetic pathway. The protein is a member of CYP90B gene family. CLM is an epi-allele with small, compressed rosette, reduced internode length, and reduced fertility, appears in selfed ddm mutant plants possibly due to loss of cytosine methylation. Transcripts accumulate in actively growing tissues, and GUS expression is negatively regulated by brassinosteroids. Localized in the endoplasmic reticulum. The in vitro expressed protein can perform the C-22 hydroxylation of a variety of C27-, C28- and C29-sterols. Cholesterol was the best substrate, followed by campesterol. Sitosterol was a poor substrate.
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Genomic context

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See DWF4 in Genome Data Viewer

Location:: chromosome: 3

Exon count:: 8

Sequence:: Chromosome: 3; NC_003074.8 (18813949..18817405, complement)

Chromosome 3 - NC_003074.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003074.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Blue light signalling was important for DWF4 accumulation in root tips and root growth. DWF4 accumulation patterns in primary root tips were not altered by auxin or sugar treatment.
Title: DWARF4 accumulation in root tips is enhanced via blue light perception by cryptochromes.
TCP1 Modulates DWF4 Expression via Directly Interacting with the GGNCCC Motifs in the Promoter Region of DWF4 in Arabidopsis thaliana
Title: TCP1 Modulates DWF4 Expression via Directly Interacting with the GGNCCC Motifs in the Promoter Region of DWF4 in Arabidopsis thaliana.
Overexpression of CYP724A1 in null dwf4-102 plants functionally complements their C-22 hydroxylase deficiency.
Title: Functional complementation of dwf4 mutants of Arabidopsis by overexpression of CYP724A1.
TCP1 positively regulates BR biosynthesis via its direct regulation of the transcription of DWF4.
Title: TCP1 positively regulates the expression of DWF4 in Arabidopsis thaliana.
DWF4 transcription plays a novel role in the brassinosteroid (BR)-auxin crosstalk associated with root elongation, in addition to its role in BR homeostasis.
Title: Transcription of DWARF4 plays a crucial role in auxin-regulated root elongation in addition to brassinosteroid homeostasis in Arabidopsis thaliana.
The binding of BZR1 (brassinazole-resistant 1 protein) to the DWF4 promoter is attenuated by treatment with auxin.
Title: Auxin stimulates DWARF4 expression and brassinosteroid biosynthesis in Arabidopsis.
Studies indicate that DWF4 gene encodes a 22-a hydroxylase (CYP90B1) that catalyzes the conversion of 6- Oxocampestanol/Campestanol to 6-Deoxocathasterone/Cathasterone.
Title: Genetic analyses of interactions among gibberellin, abscisic acid, and brassinosteroids in the control of flowering time in Arabidopsis thaliana.
TCP1 positively regulates the expression of the key brassinosteroid biosynthetic gene, DWF4.
Title: TCP1 modulates brassinosteroid biosynthesis by regulating the expression of the key biosynthetic gene DWARF4 in Arabidopsis thaliana.
Down-regulation of the DET2:DWF4:SMT2 genes in transgenic Arabidopsis leads to a selective modification of the campesterol and brassinosteroid profiles.
Title: Simultaneous suppression of three genes related to brassinosteroid (BR) biosynthesis altered campesterol and BR contents, and led to a dwarf phenotype in Arabidopsis thaliana.
Brassinosteroid negatively regulates jasmonate inhibition of root growth, and DWF4 is down-regulated by jasmonate.
Title: A leaky mutation in DWARF4 reveals an antagonistic role of brassinosteroid in the inhibition of root growth by jasmonate in Arabidopsis.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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NM_001339479.1

Supported by ESTs:

NM_114926.4

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables heme binding	IEA Inferred from Electronic Annotation more info
enables iron ion binding	IEA Inferred from Electronic Annotation more info
enables monooxygenase activity	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen	IEA Inferred from Electronic Annotation more info
enables steroid 22-alpha hydroxylase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in brassinosteroid biosynthetic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within brassinosteroid biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within jasmonic acid mediated signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within leaf development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within leaf shaping	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to brassinosteroid	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within response to brassinosteroid	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to jasmonic acid	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within unidimensional cell growth	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Cytochrome P450 superfamily protein

NP_190635.1

DWARF 4 (DWF4); CONTAINS InterPro DOMAIN/s: Cytochrome P450 (InterPro:IPR001128), Cytochrome P450, conserved site (InterPro:IPR017972), Cytochrome P450, E-class, group I (InterPro:IPR002401); BEST Arabidopsis thaliana protein match is: Cytochrome P450 superfamily protein (TAIR:AT5G05690.1); Has 31120 Blast hits to 31036 proteins in 1650 species: Archae - 61; Bacteria - 5303; Metazoa - 10591; Fungi - 5722; Plants - 7996; Viruses - 3; Other Eukaryotes - 1444 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003074.8 Reference assembly

Range

18813949..18817405 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_114926.4 → NP_190635.1 Cytochrome P450 superfamily protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_190635.1

Status: REVIEWED

UniProtKB/Swiss-Prot

O64989, Q9SCQ9

UniProtKB/TrEMBL

A0A178VC87

Conserved Domains (1) summary

PLN02500
Location:29 → 513

PLN02500; cytochrome P450 90B1
NM_001339479.1 → NP_001327730.1 Cytochrome P450 superfamily protein [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/TrEMBL

A0A178VC32, A0A1I9LT77