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    ASA1 anthranilate synthase alpha subunit 1 [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 830457, updated on 18-Sep-2024

    Summary

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    Official Symbol
    ASA1
    Official Full Name
    anthranilate synthase alpha subunit 1
    Primary source
    TAIR:AT5G05730
    Locus tag
    AT5G05730
    See related
    Araport:AT5G05730
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    A-METHYL TRYPTOPHAN RESISTANT 1; AMT1; ANTHRANILATE SYNTHASE ALPHA SUBUNIT; anthranilate synthase alpha subunit 1; JASMONATE-INDUCED DEFECTIVE LATERAL ROOT 1; JDL1; MJJ3.14; MJJ3_14; TRP5; TRYPTOPHAN BIOSYNTHESIS 5; WEAK ETHYLENE INSENSITIVE 2; WEI2
    Summary
    ASA1 encodes the alpha subunit of anthranilate synthase, which catalyzes the rate-limiting step of tryptophan synthesis. ASA1 is induced by ethylene, and forms a link between ethylene signalling and auxin synthesis in roots.
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    Genomic context

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    See ASA1 in Genome Data Viewer
    Location:
    chromosome: 5
    Exon count:
    9
    Sequence:
    Chromosome: 5; NC_003076.8 (1719533..1722933, complement)

    Chromosome 5 - NC_003076.8Genomic Context describing neighboring genes Neighboring gene arginine-tRNA protein transferase 1 Neighboring gene Pleckstrin homology (PH) domain superfamily protein Neighboring gene RNA-binding (RRM/RBD/RNP motifs) family protein Neighboring gene uncharacterized protein Neighboring gene ethylene-dependent gravitropism-deficient and yellow-green-like 2 Neighboring gene DNAJ heat shock N-terminal domain-containing protein Neighboring gene ncRNA

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003076.8

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Functional expression of Arabidopsis thaliana anthranilate synthase subunit I in Escherichia coli. Bernasconi P, et al. Plant Physiol, 1994 Sep. PMID 7972519, Free PMC Article
    2. The Arabidopsis thaliana trp5 mutant has a feedback-resistant anthranilate synthase and elevated soluble tryptophan. Li J, et al. Plant Physiol, 1996 Jan. PMID 8587994, Free PMC Article
    3. Molecular basis of alpha-methyltryptophan resistance in amt-1, a mutant of Arabidopsis thaliana with altered tryptophan metabolism. Kreps JA, et al. Plant Physiol, 1996 Apr. PMID 8934623, Free PMC Article
    4. Arabidopsis ASA1 is important for jasmonate-mediated regulation of auxin biosynthesis and transport during lateral root formation. Sun J, et al. Plant Cell, 2009 May. PMID 19435934, Free PMC Article
    5. Pore mutations in ammonium transporter AMT1 with increased electrogenic ammonium transport activity. Loqué D, et al. J Biol Chem, 2009 Sep 11. PMID 19581303, Free PMC Article

    See all (18) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. A transcription factor STOP1-centered pathway coordinates ammonium and phosphate acquisition in Arabidopsis.
      Title: A transcription factor STOP1-centered pathway coordinates ammonium and phosphate acquisition in Arabidopsis.
    2. indole-3-butyric acid induced adventitious root-formation by conversion into Indole-3-acetic acid (IAA) involving nitric oxide activity, and by a positive action on IAA-transport and ASA1/ASB1-mediated IAA-biosynthesis
      Title: Indole-3-butyric acid promotes adventitious rooting in Arabidopsis thaliana thin cell layers by conversion into indole-3-acetic acid and stimulation of anthranilate synthase activity.
    3. In BnaA.TTG2.a.1-overexpressing Arabidopsis under salt stress, the endogenous indole-3-acetic acid content was reduced, and expression of two auxin biosynthesis genes, TRYPTOPHAN BIOSYNTHESIS 5 (TRP5) and YUCCA2 (YUC2), was downregulated.
      Title: Expression of Brassica napus TTG2, a regulator of trichome development, increases plant sensitivity to salt stress by suppressing the expression of auxin biosynthesis genes.
    4. Using loss- and gain-of-function transgenic lines as well as biochemical analysis, we demonstrate that ERF1 can directly up-regulate ASA1 by binding to its promoter, leading to auxin accumulation and ethylene-induced inhibition of root growth.
      Title: Arabidopsis ERF1 Mediates Cross-Talk between Ethylene and Auxin Biosynthesis during Primary Root Elongation by Regulating ASA1 Expression.
    5. Mutations of ASA1 and the TIR1/AFBs auxin receptor genes impair the inhibitory effect of 5 muM MeJA on PIN2 endocytosis and enhance the reduction effect of 50 muM MeJA on the plasma membrane accumulation of PIN2.
      Title: Jasmonate modulates endocytosis and plasma membrane accumulation of the Arabidopsis PIN2 protein.
    6. Pore mutations in ammonium transporter AMT1 may have a role in increased electrogenic ammonium transport activity
      Title: Pore mutations in ammonium transporter AMT1 with increased electrogenic ammonium transport activity.
    7. ASA1 has a role in jasmonate-induced auxin biosynthesis.
      Title: Arabidopsis ASA1 is important for jasmonate-mediated regulation of auxin biosynthesis and transport during lateral root formation.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    NM_001203302.1
    NM_120655.4

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables anthranilate synthase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables anthranilate synthase activity IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    enables anthranilate synthase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within auxin biosynthetic process IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within lateral root formation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of auxin biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within response to bacterium IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within response to ethylene IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within response to wounding IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    involved_in tryptophan biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in tryptophan biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within tryptophan biosynthetic process TAS
    Traceable Author Statement
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of anthranilate synthase complex NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    located_in chloroplast HDA PubMed 
    located_in chloroplast ISM
    Inferred from Sequence Model
    more info
    		  
    located_in chloroplast TAS
    Traceable Author Statement
    more info
    		 PubMed 
    located_in chloroplast stroma HDA PubMed 

    General protein information

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    Preferred Names
    anthranilate synthase alpha subunit 1
    NP_001190231.1
    • anthranilate synthase alpha subunit 1 (ASA1); CONTAINS InterPro DOMAIN/s: Anthranilate synthase component I, N-terminal (InterPro:IPR006805), Chorismate binding, C-terminal (InterPro:IPR015890), ADC synthase (InterPro:IPR005801), Anthranilate synthase component I (InterPro:IPR019999), Anthranilate synthase component I, PabB-like (InterPro:IPR005256); BEST Arabidopsis thaliana protein match is: anthranilate synthase 2 (TAIR:AT2G29690.1).
    NP_196192.1
    • anthranilate synthase alpha subunit 1 (ASA1); FUNCTIONS IN: anthranilate synthase activity; INVOLVED IN: in 6 processes; LOCATED IN: chloroplast, anthranilate synthase complex; EXPRESSED IN: 27 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Anthranilate synthase component I, N-terminal (InterPro:IPR006805), Chorismate binding, C-terminal (InterPro:IPR015890), ADC synthase (InterPro:IPR005801), Anthranilate synthase component I (InterPro:IPR019999), Anthranilate synthase component I, PabB-like (InterPro:IPR005256); BEST Arabidopsis thaliana protein match is: anthranilate synthase 2 (TAIR:AT2G29690.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003076.8 Reference assembly

      Range
      1719533..1722933 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_120655.4 → NP_196192.1  anthranilate synthase alpha subunit 1 [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_196192.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      P32068
      UniProtKB/TrEMBL
      A0A178UPV8, A0A5S9Y1Q1
      Conserved Domains (3) summary
      PLN02445
      Location:72 → 594
      PLN02445; anthranilate synthase component I
      pfam00425
      Location:307 → 577
      Chorismate_bind; chorismate binding enzyme
      pfam04715
      Location:89 → 245
      Anth_synt_I_N; Anthranilate synthase component I, N terminal region

    2. NM_001203302.1 → NP_001190231.1  anthranilate synthase alpha subunit 1 [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_001190231.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A178UPV8, A0A5S9Y1Q1, F4K0T5
      Conserved Domains (3) summary
      PLN02445
      Location:72 → 623
      PLN02445; anthranilate synthase component I
      pfam00425
      Location:307 → 606
      Chorismate_bind; chorismate binding enzyme
      pfam04715
      Location:89 → 245
      Anth_synt_I_N; Anthranilate synthase component I, N terminal region
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P32068.1

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