CLPB3 casein lytic proteinase B3 [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: CLPB3
Official Full Name: casein lytic proteinase B3
Primary source: TAIR:AT5G15450
Locus tag: AT5G15450
See related: Araport:AT5G15450
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: ALBINO AND PALE GREEN 6; APG6; AtCLPB3; CASEIN LYTIC PROTEINASE B-P; casein lytic proteinase B3; CLPB-P; T20K14.60; T20K14_60
Summary: Encodes a chloroplast-targeted Hsp101 homologue. Functions as a molecular chaperone involved in plastid differentiation mediating internal thylakoid membrane formation and conferring thermotolerance to chloroplasts during heat stress. APG6 is constitutively expressed in the root tips, the organ boundary region, the reproductive tissues of mature plants where plastids exist as proplastids, and slightly in the stems and leaves. APG6 expression is upregulated in response to heat shock in various organs, but not in response to other abiotic stresses. Apg6 mutants have a pale-green phenotype.
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Genomic context

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See CLPB3 in Genome Data Viewer

Location:: chromosome: 5

Exon count:: 9

Sequence:: Chromosome: 5; NC_003076.8 (5014212..5018347, complement)

Chromosome 5 - NC_003076.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003076.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Biochemical characterization of ClpB3, a chloroplastic disaggregase from Arabidopsis thaliana.
Title: Biochemical characterization of ClpB3, a chloroplastic disaggregase from Arabidopsis thaliana.
LIN treatment rapidly increases the levels of aggregated proteins in the chloroplast, unleashing a specific retrograde signaling pathway that up-regulates expression of ClpB3 and other nuclear genes encoding plastidial chaperones.Expression of HsfA2 and cpUPR-related target genes is independent of GUN1, a central integrator of retrograde signaling pathways
Title: Interference with plastome gene expression and Clp protease activity in Arabidopsis triggers a chloroplast unfolded protein response to restore protein homeostasis.
We conclude that specific J-proteins and Hsp100 chaperones act together with Hsp70 to recognize and deliver DXS to either reactivation (via ClpB3) or removal (via ClpC1) depending on the physiological status of the plastid.
Title: Specific Hsp100 Chaperones Determine the Fate of the First Enzyme of the Plastidial Isoprenoid Pathway for Either Refolding or Degradation by the Stromal Clp Protease in Arabidopsis.
CLPB3 co-expressed tightly with genes coding for PAP1, GBF6 and bHLH family member transcription factors during the acclimation.
Title: The irradiance dependent transcriptional regulation of AtCLPB3 expression.
Data show that chloroplast unfoldase ClpB3 was strongly up-regulated in both young and mature leaves, suggesting widespread and persistent protein folding stress.
Title: Large scale comparative proteomics of a chloroplast Clp protease mutant reveals folding stress, altered protein homeostasis, and feedback regulation of metabolism.
APG6 functions as a molecular chaperone involved in plastid differentiation mediating internal thylakoid membrane formation and conferring thermotolerance to chloroplasts during heat stress. [APG6]
Title: An Arabidopsis chloroplast-targeted Hsp101 homologue, APG6, has an essential role in chloroplast development as well as heat-stress response.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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NM_121549.3

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within chloroplast organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein refolding	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within response to heat	IEP Inferred from Expression Pattern more info	PubMed

Component	Evidence Code	Pubs
located_in chloroplast	HDA more info	PubMed
located_in chloroplast	IDA Inferred from Direct Assay more info	PubMed
located_in chloroplast stroma	HDA more info	PubMed
located_in cytoplasm	ISM Inferred from Sequence Model more info
located_in plastid	HDA more info	PubMed
located_in plastid stroma	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: casein lytic proteinase B3

NP_568314.1

casein lytic proteinase B3 (CLPB3); FUNCTIONS IN: nucleoside-triphosphatase activity, ATPase activity, nucleotide binding, ATP binding; INVOLVED IN: chloroplast organization, response to heat; LOCATED IN: plastid stroma, chloroplast, chloroplast stroma; EXPRESSED IN: 28 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Clp ATPase, C-terminal (InterPro:IPR019489), ATPase, AAA+ type, core (InterPro:IPR003593), ATPase, AAA-2 (InterPro:IPR013093), ATPase, AAA-type, core (InterPro:IPR003959), Chaperonin clpA/B (InterPro:IPR001270), Chaperonin ClpB (InterPro:IPR017730), Chaperonin ClpA/B, conserved site (InterPro:IPR018368), Clp, N-terminal (InterPro:IPR004176); BEST Arabidopsis thaliana protein match is: casein lytic proteinase B4 (TAIR:AT2G25140.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).