Fmnl3 formin-like 3 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Fmnl3provided by MGI
Official Full Name: formin-like 3provided by MGI
Primary source: MGI:MGI:109569
See related: Ensembl:ENSMUSG00000023008 AllianceGenome:MGI:109569
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Wbp3; FBP11; mKIAA2014; 2700073B04Rik
Summary: Enables GTPase activating protein binding activity. Predicted to be involved in several processes, including cortical actin cytoskeleton organization; filopodium assembly; and regulation of cell shape. Predicted to be located in Golgi apparatus and plasma membrane. Predicted to be active in cytosol. Is expressed in several structures, including alimentary system; cardiovascular system; genitourinary system; nervous system; and retina. Orthologous to human FMNL3 (formin like 3). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in lung adult (RPKM 22.2), ovary adult (RPKM 18.2) and 27 other tissues See more
Orthologs: human all
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Genomic context

Location:: 15 F1; 15 56.13 cM

Exon count:: 28

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	15	NC_000081.7 (99215091..99268375, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	15	NC_000081.6 (99317210..99370521, complement)

Chromosome 15 - NC_000081.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Our data thus link FMNL2/3 formins to actin assembly-dependent functions of Cdc42 in anterograde transport through the Golgi apparatus.
Title: FMNL2 and -3 regulate Golgi architecture and anterograde transport downstream of Cdc42.
force generation in lamellipodia strongly depends on FMNL formin activity.
Title: FMNL formins boost lamellipodial force generation.
FMNL3 functions in assembly of actin-based protrusions that are specialized for cell-cell adhesion.
Title: The formin FMNL3 assembles plasma membrane protrusions that participate in cell-cell adhesion.
3.4-A structure of FMNL3 FH2 dimer in complex with tetramethylrhodamine-actin uncovers details of formin-regulated actin elongation; structure provides details for FH2-mediated filament elongation by processive capping and supports a model in which C-terminal non-FH2 residues of FMNL3 are required to stabilize the filament nucleus
Title: FMNL3 FH2-actin structure gives insight into formin-mediated actin nucleation and elongation.
The C terminus of formin FMNL3 accelerates actin polymerization and contains a WH2 domain-like sequence that binds both monomers and filament barbed ends.
Title: The C terminus of formin FMNL3 accelerates actin polymerization and contains a WH2 domain-like sequence that binds both monomers and filament barbed ends.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Targeted (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D15Mit263 (e-PCR), detects polymorphism
- UniSTS:127636

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables GTPase activating protein binding	IDA Inferred from Direct Assay more info	PubMed
enables actin binding	IEA Inferred from Electronic Annotation more info
enables actin filament binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables small GTPase binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in actin cytoskeleton organization	IEA Inferred from Electronic Annotation more info
involved_in actin cytoskeleton organization	ISO Inferred from Sequence Orthology more info
involved_in angiogenesis	IEA Inferred from Electronic Annotation more info
involved_in cell migration	IBA Inferred from Biological aspect of Ancestor more info
involved_in cell migration	ISO Inferred from Sequence Orthology more info
involved_in cell migration	ISS Inferred from Sequence or Structural Similarity more info
involved_in cortical actin cytoskeleton organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in cytoskeleton organization	ISO Inferred from Sequence Orthology more info
involved_in cytoskeleton organization	ISS Inferred from Sequence or Structural Similarity more info
involved_in filopodium assembly	IEA Inferred from Electronic Annotation more info
involved_in filopodium assembly	ISO Inferred from Sequence Orthology more info
involved_in regulation of cell shape	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of cell shape	ISO Inferred from Sequence Orthology more info
involved_in regulation of cell shape	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
located_in Golgi apparatus	IEA Inferred from Electronic Annotation more info
located_in Golgi apparatus	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in intracellular membrane-bounded organelle	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: formin-like protein 3

Names: WW domain binding protein 3; formin-like 3 protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001310622.1 → NP_001297551.1 formin-like protein 3 isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks the penultimate exon compared to variant 1, which results in a frame-shift and a shorter isoform (2) with a distinct C-terminus compared to isoform 1.

Source sequence(s)

AC118646, AK163361, AU042388, BC131961

Consensus CDS

CCDS79409.1

UniProtKB/TrEMBL

D3Z7A7

Related

ENSMUSP00000113094.2, ENSMUST00000120633.2

Conserved Domains (3) summary

pfam02181
Location:561 → 926

FH2; Formin Homology 2 Domain

pfam06367
Location:281 → 472

Drf_FH3; Diaphanous FH3 Domain

pfam06371
Location:26 → 278

Drf_GBD; Diaphanous GTPase-binding Domain
NM_001310623.1 → NP_001297552.1 formin-like protein 3 isoform 3

See identical proteins and their annotated locations for NP_001297552.1

Status: VALIDATED

Description

Transcript Variant: This variant (3) lacks an in-frame coding exon in the mid region and the penultimate exon compared to variant 1. The resulting isoform (3) is shorter missing an internal protein segment and with a distinct C-terminus compared to isoform 1.

Source sequence(s)

AC118646, AU042388, BC131961

Consensus CDS

CCDS79408.1

UniProtKB/Swiss-Prot

Q6ZPF4

Related

ENSMUSP00000079984.8, ENSMUST00000081224.14

Conserved Domains (3) summary

pfam02181
Location:510 → 875

FH2; Formin Homology 2 Domain

pfam06367
Location:230 → 421

Drf_FH3; Diaphanous FH3 Domain

pfam06371
Location:26 → 227

Drf_GBD; Diaphanous GTPase-binding Domain
NM_011711.2 → NP_035841.1 formin-like protein 3 isoform 1

See identical proteins and their annotated locations for NP_035841.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longest isoform (1).

Source sequence(s)

AC118646, AU042388, BC131961

Consensus CDS

CCDS27819.1

UniProtKB/Swiss-Prot

Q3TCF9, Q3U2E4, Q6ZPF4

Related

ENSMUSP00000085566.7, ENSMUST00000088233.13

Conserved Domains (3) summary

pfam02181
Location:561 → 926

FH2; Formin Homology 2 Domain

pfam06367
Location:281 → 472

Drf_FH3; Diaphanous FH3 Domain

pfam06371
Location:26 → 278

Drf_GBD; Diaphanous GTPase-binding Domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000081.7 Reference GRCm39 C57BL/6J

Range

99215091..99268375 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006520844.5 → XP_006520907.1 formin-like protein 3 isoform X1

Conserved Domains (3) summary

pfam02181
Location:510 → 875

FH2; Formin Homology 2 Domain

pfam06367
Location:230 → 421

Drf_FH3; Diaphanous FH3 Domain

pfam06371
Location:26 → 227

Drf_GBD; Diaphanous GTPase-binding Domain
XM_030248512.2 → XP_030104372.1 formin-like protein 3 isoform X2

Conserved Domains (3) summary

pfam02181
Location:327 → 692

FH2; Formin Homology 2 Domain

pfam06367
Location:47 → 238

Drf_FH3; Diaphanous FH3 Domain

pfam06371
Location:1 → 44

Drf_GBD; Diaphanous GTPase-binding Domain