nsfa N-ethylmaleimide-sensitive factor a [Danio rerio (zebrafish)] - Gene

Summary

Official Symbol: nsfaprovided by ZNC
Official Full Name: N-ethylmaleimide-sensitive factor aprovided by ZNC
Primary source: ZFIN:ZDB-GENE-030616-37
See related: Ensembl:ENSDARG00000007654 AllianceGenome:ZFIN:ZDB-GENE-030616-37
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Danio rerio
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; Danionidae; Danioninae; Danio
Also known as: nsf; wu:fj33g11; si:bz18k17.1
Summary: Predicted to enable ATP binding activity; ATP hydrolysis activity; and metal ion binding activity. Acts upstream of or within several processes, including hypothalamus development; neurogenesis; and swim bladder development. Predicted to be located in cytoplasm. Is expressed in several structures, including nervous system; neural rod; neural tube; swim bladder; and trigeminal placode. Human ortholog(s) of this gene implicated in developmental and epileptic encephalopathy 96. Orthologous to human NSF (N-ethylmaleimide sensitive factor, vesicle fusing ATPase). [provided by Alliance of Genome Resources, Jan 2025]
Orthologs: all
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Genomic context

Location:: chromosome: 3

Exon count:: 22

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCz11 (GCF_000002035.6)	3	NC_007114.7 (34766987..34801041, complement)
105	previous assembly	GRCz10 (GCF_000002035.5)	3	NC_007114.6 (34637479..34671533, complement)

Chromosome 3 - NC_007114.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
Description: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
BioProject: PRJEB1986
Analysis date: Fri Dec 8 19:48:10 2017

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Molecular analysis of ndrg4 mutants shows that expression of snap25 and nsf are sharply decreased, revealing a role of ndrg4 in controlling vesicle exocytosis. This uncovers a previously unknown function of ndrg4 in regulating vesicle docking and nodes of Ranvier organization, at least through its ability to finely tune the expression of the t-SNARE/NSF machinery.
Title: Neuronal Ndrg4 Is Essential for Nodes of Ranvier Organization in Zebrafish.
results establish a role for Nsf in the maintenance of synaptic contacts between hair cells and afferent neurons, mediated in part via the secretion of trophic signaling factors
Title: Both pre- and postsynaptic activity of Nsf prevents degeneration of hair-cell synapses.
This study analyses of nsf mutant zebrafish reveal an unexpected role for NSF in hypothalamic development, with mutant 5 days post-fertilization larvae exhibiting a stage-dependent loss of neuroendocrine transcripts.
Title: Neuroendocrine transcriptional programs adapt dynamically to the supply and demand for neuropeptides as revealed in NSF mutant zebrafish.
Activation of PKCgamma is necessary for the activity-dependent trafficking of AMPARs in embryonic zebrafish and is NMDA and SNARE-dependent and requires AMPARs to associate with both NSF and PICK1.
Title: PKCgamma-induced trafficking of AMPA receptors in embryonic zebrafish depends on NSF and PICK1.
Nsf is essential in the formation of the nodes of Ranvier in the vertebrate nervous system.
Title: nsf is essential for organization of myelinated axons in zebrafish.

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General gene information

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Gene Ontology Provided by ZFIN

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within SNARE complex disassembly	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within Schwann cell development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within Schwann cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within afferent axon development in posterior lateral line nerve	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within clustering of voltage-gated sodium channels	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within efferent axon development in posterior lateral line nerve	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within eye morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within hypothalamus development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within myelination of anterior lateral line nerve axons	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within myelination of posterior lateral line nerve axons	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within pigment granule localization	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within protein transport	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within swim bladder development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within vesicle-mediated transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: vesicle-fusing ATPase

NP_001037793.1

EC 3.6.4.6

XP_021325418.1

EC 3.6.4.6

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001044328.1 → NP_001037793.1 vesicle-fusing ATPase

See identical proteins and their annotated locations for NP_001037793.1

Status: PROVISIONAL

Source sequence(s)

AL590145

UniProtKB/TrEMBL

B7ZV62, B7ZVV4

Related

ENSDARP00000093817.5, ENSDART00000103043.6

Conserved Domains (5) summary

smart00382
Location:254 → 399

AAA; ATPases associated with a variety of cellular activities

smart01072
Location:111 → 183

CDC48_2; Cell division protein 48 (CDC48) domain 2

pfam00004
Location:256 → 397

AAA; ATPase family associated with various cellular activities (AAA)

pfam02359
Location:5 → 85

CDC48_N; Cell division protein 48 (CDC48), N-terminal domain

cl21455
Location:539 → 652

P-loop_NTPase; P-loop containing Nucleoside Triphosphate Hydrolases

RefSeqs of Annotated Genomes: GCF_000002035.6-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCz11 Primary Assembly

Genomic

NC_007114.7 Reference GRCz11 Primary Assembly

Range

34766987..34801041 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_021469743.2 → XP_021325418.1 vesicle-fusing ATPase isoform X1

UniProtKB/TrEMBL

A0A8M9PEX3

Conserved Domains (3) summary

smart01072
Location:111 → 183

CDC48_2; Cell division protein 48 (CDC48) domain 2

smart01073
Location:6 → 83

CDC48_N; Cell division protein 48 (CDC48) N-terminal domain

cl27568
Location:160 → 606

TIP49; TIP49 C-terminus