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    pldA outer membrane phospholipase A [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948307, updated on 3-Dec-2024

    Summary

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    Official Symbol
    pldA
    Official Full Name
    outer membrane phospholipase A
    Primary source
    ECOCYC:EG10738
    Locus tag
    b3821
    See related
    ASAP:ABE-0012479
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3815
    Summary
    PldA is the Phospholipase A1 precursor which is a member of EC 3.1.1.32. [More information is available at EcoCyc: EG10738].
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    Genomic context

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    Sequence:
    NC_000913.3 (4004862..4005731)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene putative transporter RarD Neighboring gene putative thioesterase YigI Neighboring gene ATP-dependent DNA helicase RecQ Neighboring gene L-threonine exporter

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Dimerization regulates the enzymatic activity of Escherichia coli outer membrane phospholipase A. Dekker N, et al. J Biol Chem, 1997 Feb 7. PMID 9013551
    2. Outer-membrane phospholipase A: known structure, unknown biological function. Dekker N. Mol Microbiol, 2000 Feb. PMID 10692149
    3. Structural evidence for dimerization-regulated activation of an integral membrane phospholipase. Snijder HJ, et al. Nature, 1999 Oct 14. PMID 10537112
    4. Outer membrane phospholipase A is dimeric in phospholipid bilayers: a cross-linking and fluorescence resonance energy transfer study. Ubarretxena-Belandia I, et al. Biochemistry, 1999 Jun 1. PMID 10353852
    5. Bacteriocin release protein triggers dimerization of outer membrane phospholipase A in vivo. Dekker N, et al. J Bacteriol, 1999 May. PMID 10322034, Free PMC Article

    See all (49) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Upon the addition of Ca(2+), OmpLA was activated and degraded the membrane with a turnover of ~2 phospholipid molecules per second and per OmpLA dimer until most of the membrane phospholipids were hydrolyzed and the protein became tightly packed.
      Title: Real-time Visualization of Phospholipid Degradation by Outer Membrane Phospholipase A using High-Speed Atomic Force Microscopy.
    2. The crystal structure of the dimeric state of the transmembrane beta-barrel protein outer membrane phospholipase A (OMPLA) revealed an intermolecular hydrogen bond mediated by a highly conserved glutamine side-chain (Q94).
      Title: The role of a hydrogen bonding network in the transmembrane beta-barrel OMPLA.
    3. N-terminus verified by Edman degradation on mature peptide
      Title: The DNA sequence encoding pldA gene, the structural gene for detergent-resistant phospholipase A of E. coli.
    4. These energetic measurements not only provide insight into the activation of OMPLA, but they also represent the first quantitative investigation of the association energetics of a transmembrane beta-barrel.
      Title: Energetics of outer membrane phospholipase A (OMPLA) dimerization.
    Submit: New GeneRIF Correction

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables calcium ion binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables calcium ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables calcium-dependent phospholipase A2 activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables calcium-independent phospholipase A2 activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables lysophospholipase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables phosphatidylserine 1-acylhydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables phospholipase A1 activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables phospholipase A1 activity TAS
    Traceable Author Statement
    more info
    		 PubMed 
    enables phospholipase A2 activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables phospholipase A2 activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables phospholipase A2 activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables phospholipase A2 activity TAS
    Traceable Author Statement
    more info
    		 PubMed 
    enables phospholipase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables phospholipase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein homodimerization activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in lipid catabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in lipid metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within phosphatidylglycerol metabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    is_active_in cell outer membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cell outer membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cell outer membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    outer membrane phospholipase A
    NP_418265.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      4004862..4005731
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418265.1 outer membrane phospholipase A [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418265.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      C3SK99
      Conserved Domains (1) summary
      PRK10763
      Location:1 → 289
      PRK10763; phospholipase A; Provisional
    Nucleotide Protein
    Heading Accession and Version

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