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    kdsA 3-deoxy-D-manno-octulosonate 8-phosphate synthase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945785, updated on 3-Dec-2024

    Summary

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    Official Symbol
    kdsA
    Official Full Name
    3-deoxy-D-manno-octulosonate 8-phosphate synthase
    Primary source
    ECOCYC:EG10518
    Locus tag
    b1215
    See related
    ASAP:ABE-0004077
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK1203
    Summary
    kdsA gutQ double mutants are non-viable and lack KDO; multicopy msbA, or unknown chromsomal mutation(s) in suppressed KDO-null strain KPM22, permit viabilty without KDO-containing LPS (Meredith, 2006). [More information is available at EcoGene: EG10518]. 3-deoxy-D-manno-octulosonate 8-phosphate (KDO-8-P) synthase is a key enzyme in lipopolysaccharide biosynthesis. [More information is available at EcoCyc: EG10518].
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    Genomic context

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    Sequence:
    NC_000913.3 (1268165..1269019)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene protein-(glutamine-N(5)) methyltransferase Neighboring gene SirB family protein YchQ Neighboring gene transglutaminase-like/TPR repeat-containing protein Neighboring gene small toxic polypeptide LdrA Neighboring gene ncRNA

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Catalytic mechanism of 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase. The use of synthetic analogues to probe the structure of the putative reaction intermediate. Baasov T, et al. Eur J Biochem, 1993 Nov 1. PMID 8223657
    2. Structure and mechanism of 3-deoxy-D-manno-octulosonate 8-phosphate synthase. Radaev S, et al. J Biol Chem, 2000 Mar 31. PMID 10734095
    3. Preliminary X-ray analysis of a new crystal form of the Escherichia coli KDO8P synthase. Radaev S, et al. Acta Crystallogr D Biol Crystallogr, 2000 Apr. PMID 10739938
    4. 3-Deoxy-D-manno-octulosonate-8-phosphate synthase from Escherichia coli. Model of binding of phosphoenolpyruvate and D-arabinose-5-phosphate. Wagner T, et al. J Mol Biol, 2000 Aug 11. PMID 10926505
    5. Crystal structures of KDOP synthase in its binary complexes with the substrate phosphoenolpyruvate and with a mechanism-based inhibitor. Asojo O, et al. Biochemistry, 2001 May 29. PMID 11371194

    See all (41) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. N-terminus verified by Edman degradation on complete protein
      Title: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.
    2. a mutation affects the metal requirement of 3-deoxy-D-manno-2-octulosonate-8-phosphate (KDO8P) synthases from Aquifex pyrophilus and Escherichia coli
      Title: A reciprocal single mutation affects the metal requirement of 3-deoxy-D-manno-2-octulosonate-8-phosphate (KDO8P) synthases from Aquifex pyrophilus and Escherichia coli.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables 3-deoxy-8-phosphooctulonate synthase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables 3-deoxy-8-phosphooctulonate synthase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables 3-deoxy-8-phosphooctulonate synthase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in keto-3-deoxy-D-manno-octulosonic acid biosynthetic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within keto-3-deoxy-D-manno-octulosonic acid biosynthetic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in keto-3-deoxy-D-manno-octulosonic acid biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in lipopolysaccharide biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol HDA PubMed 
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of protein-containing complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    3-deoxy-D-manno-octulosonate 8-phosphate synthase
    NP_415733.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1268165..1269019
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415733.1 3-deoxy-D-manno-octulosonate 8-phosphate synthase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415733.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A1V2T1J3, A0A2W6PFE8, A0A775X2E9
      Conserved Domains (1) summary
      COG2877
      Location:3 → 282
      KdsA; 3-deoxy-D-manno-octulosonic acid (KDO) 8-phosphate synthase [Cell wall/membrane/envelope biogenesis]
    Nucleotide Protein
    Heading Accession and Version

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