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    DAT Dopamine transporter [ Drosophila melanogaster (fruit fly) ]

    Gene ID: 36849, updated on 9-Dec-2024

    Summary

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    Official Symbol
    DATprovided by FlyBase
    Official Full Name
    Dopamine transporterprovided by FlyBase
    Primary source
    FLYBASE:FBgn0034136
    Locus tag
    Dmel_CG8380
    See related
    AllianceGenome:FB:FBgn0034136
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Drosophila melanogaster
    Lineage
    Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
    Also known as
    CG8380; dat; Dat; dDat; dDAT; DmDAT; Dmel\CG8380; fmn; fumin; Fumin
    Summary
    Enables cocaine binding activity and dopamine:sodium symporter activity. Involved in several processes, including dopamine transport; regulation of presynaptic cytosolic calcium ion concentration; and response to odorant. Located in plasma membrane. Is expressed in several structures, including anlage in statu nascendi; anterior-posterior subdivision of organism; dopaminergic neurons; mushroom body slice; and synaptic neuropil domain. Used to study attention deficit hyperactivity disorder; autism spectrum disorder; and classic dopamine transporter deficiency syndrome. Human ortholog(s) of this gene implicated in several diseases, including alcohol dependence; attention deficit hyperactivity disorder; classic dopamine transporter deficiency syndrome; mild cognitive impairment; and neurocirculatory asthenia. Orthologous to human SLC6A2 (solute carrier family 6 member 2) and SLC6A3 (solute carrier family 6 member 3). [provided by Alliance of Genome Resources, Dec 2024]
    Orthologs
    all
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    Genomic context

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    See DAT in Genome Data Viewer
    Location:
    53C7-53C8; 2-80 cM
    Exon count:
    8
    Annotation release Status Assembly Chr Location
    Release 6.54 current Release 6 plus ISO1 MT (GCF_000001215.4) 2R NT_033778.4 (16558567..16565238, complement)
    Release 5.57 previous assembly Release 5 (GCF_000001215.2) 2R NT_033778.3 (12446072..12452743, complement)

    Chromosome 2R - NT_033778.4Genomic Context describing neighboring genes Neighboring gene loopin-1 Neighboring gene Syntrophin-like 2 Neighboring gene uncharacterized protein Neighboring gene Ribosomal protein S15 Neighboring gene uncharacterized protein

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Flipped Phenyl Ring Orientations of Dopamine Binding with Human and Drosophila Dopamine Transporters: Remarkable Role of Three Nonconserved Residues. Yuan Y, et al. ACS Chem Neurosci, 2018 Jun 20. PMID 29494767, Free PMC Article
    2. Guidelines for Homology Modeling of Dopamine, Norepinephrine, and Serotonin Transporters. Haddad Y, et al. ACS Chem Neurosci, 2016 Nov 16. PMID 27596073
    3. Amphetamine-induced behavior requires CaMKII-dependent dopamine transporter phosphorylation. Pizzo AB, et al. Mol Psychiatry, 2014 Mar. PMID 23508128, Free PMC Article
    4. When transporters fail to be transported: how to rescue folding-deficient SLC6 transporters. Sucic S, et al. J Neurol Neuromedicine, 2016 Dec 30. PMID 28405636, Free PMC Article
    5. Using In Vivo Electrochemistry to Study the Physiological Effects of Cocaine and Other Stimulants on the Drosophila melanogaster Dopamine Transporter. Makos MA, et al. ACS Chem Neurosci, 2010 Jan 20. PMID 20352129, Free PMC Article

    See all (218) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Dopamine reuptake and inhibitory mechanisms in human dopamine transporter.
      Title: Dopamine reuptake and inhibitory mechanisms in human dopamine transporter.
    2. Structure of the human dopamine transporter and mechanisms of inhibition.
      Title: Structure of the human dopamine transporter and mechanisms of inhibition.
    3. Pharmacological Characterization of Purified Full-Length Dopamine Transporter from Drosophila melanogaster.
      Title: Pharmacological Characterization of Purified Full-Length Dopamine Transporter from Drosophila melanogaster.
    4. Dopaminergic Ric GTPase activity impacts amphetamine sensitivity and sleep quality in a dopamine transporter-dependent manner in Drosophila melanogaster.
      Title: Dopaminergic Ric GTPase activity impacts amphetamine sensitivity and sleep quality in a dopamine transporter-dependent manner in Drosophila melanogaster.
    5. Structural Determinants of the Dopamine Transporter Regulation Mediated by G Proteins.
      Title: Structural Determinants of the Dopamine Transporter Regulation Mediated by G Proteins.
    6. Identification of a New Allosteric Binding Site for Cocaine in Dopamine Transporter.
      Title: Identification of a New Allosteric Binding Site for Cocaine in Dopamine Transporter.
    7. Structural basis of norepinephrine recognition and transport inhibition in neurotransmitter transporters.
      Title: Structural basis of norepinephrine recognition and transport inhibition in neurotransmitter transporters.
    8. Study identified Na+- and dopamine-induced changes in specific regions of the wild-type Drosophila melanogaster dopamine transporter, suggesting their involvement in protein conformational transitions. Ligand-dependent slow cooperative fluctuations of helical stretches in several domains of the transporter were detected, which could be a molecular mechanism that assists in the transporter function.
      Title: Substrate-induced conformational dynamics of the dopamine transporter.
    9. When DAT, a member of the post-feeding reward system, is either overexpressed or reduced via mutation, Drosophila has increased sensitivity to amino acid starvation.
      Title: Manipulation of components that control feeding behavior in Drosophila melanogaster increases sensitivity to amino acid starvation.
    10. X-ray crystal structures of dopamine transporter (DAT) bound to its substrate dopamine, a substrate analogue 3,4-dichlorophenethylamine, the psychostimulants d-amphetamine and methamphetamine, or to cocaine and cocaine analogues
      Title: Neurotransmitter and psychostimulant recognition by the dopamine transporter.
    Submit: New GeneRIF Correction See all GeneRIFs (14)

    Pathways from PubChem

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    General gene information

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    Homology

    Gene Ontology Provided by FlyBase

    Function Evidence Code Pubs
    enables cocaine binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables dopamine:sodium symporter activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables dopamine:sodium symporter activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in adult locomotory behavior IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in amino acid transport IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in circadian sleep/wake cycle IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in circadian sleep/wake cycle IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in dopamine transport IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in dopamine uptake involved in synaptic transmission IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in norepinephrine transport IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in regulation of presynaptic cytosolic calcium ion concentration IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in response to odorant IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in sleep IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in sodium ion transmembrane transport IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Component Evidence Code Pubs
    is_active_in axon IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in neuronal cell body membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    is_active_in plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane ISM
    Inferred from Sequence Model
    more info
    		 PubMed 
    is_active_in presynaptic membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  

    General protein information

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    Preferred Names
    dopamine transporter
    Names
    CG8380-PA
    CG8380-PB
    DAT-PA
    DAT-PB
    fumin

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NT_033778.4 Reference assembly

      Range
      16558567..16565238 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_079039.4 → NP_523763.2  dopamine transporter, isoform A [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_523763.2

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      Q7K4Y6, Q9NB97
      Related
      FBpp0086271
      Conserved Domains (1) summary
      cd11556
      Location:27 → 597
      SLC6sbd_SERT-like_u1; uncharacterized subgroup of the SERT-like Na(+)- and Cl(-)-dependent monoamine transporter subfamily; solute binding domain

    2. NM_001274097.2 → NP_001261026.1  dopamine transporter, isoform B [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001261026.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A0B4KEX2
      Conserved Domains (1) summary
      cd11556
      Location:27 → 597
      SLC6sbd_SERT-like_u1; uncharacterized subgroup of the SERT-like Na(+)- and Cl(-)-dependent monoamine transporter subfamily; solute binding domain
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    A0A0B4KEX2 A0A0B4KEX2
    Q7K4Y6.1 Q7K4Y6

    Gene LinkOut

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