Cyb5r4 cytochrome b5 reductase 4 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Cyb5r4provided by RGD
Official Full Name: cytochrome b5 reductase 4provided by RGD
Primary source: RGD:621834
See related: EnsemblRapid:ENSRNOG00000010024 AllianceGenome:RGD:621834
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Ncb5or; b5&b5R; b5+b5R; cb5/cb5R
Summary: Enables NADPH-hemoprotein reductase activity; flavin adenine dinucleotide binding activity; and heme binding activity. Involved in NADP metabolic process. Predicted to be located in perinuclear region of cytoplasm. Predicted to be active in endoplasmic reticulum. Human ortholog(s) of this gene implicated in type 2 diabetes mellitus. Orthologous to human CYB5R4 (cytochrome b5 reductase 4). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in Kidney (RPKM 180.1), Thymus (RPKM 149.1) and 9 other tissues See more
Orthologs: human all
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Genomic context

Location:: 8q31

Exon count:: 16

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	8	NC_086026.1 (96860415..96925789)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	8	NC_051343.1 (87975596..88048356)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	8	NC_005107.4 (94693273..94757989)

Chromosome 8 - NC_086026.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

G179 was required for both effective NADH/NADPH selectivity & to maintain the correct orientation & position of the conserved cysteine in the proline-rich "CGpppM" motif that is critical for optimum NADH binding & efficient hydride transfer in cb5r.
Title: Mutagenesis of Glycine 179 modulates both catalytic efficiency and reduced pyridine nucleotide specificity in cytochrome b5 reductase.

Submit: New GeneRIF Correction

General gene information

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Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables NAD(P)H oxidase H2O2-forming activity	IEA Inferred from Electronic Annotation more info
NOT enables NAD(P)H oxidase H2O2-forming activity	ISO Inferred from Sequence Orthology more info
enables NAD(P)H oxidase H2O2-forming activity	ISO Inferred from Sequence Orthology more info
enables NADPH-hemoprotein reductase activity	IDA Inferred from Direct Assay more info	PubMed
enables cytochrome-b5 reductase activity, acting on NAD(P)H	IBA Inferred from Biological aspect of Ancestor more info
enables cytochrome-b5 reductase activity, acting on NAD(P)H	IEA Inferred from Electronic Annotation more info
enables cytochrome-b5 reductase activity, acting on NAD(P)H	ISO Inferred from Sequence Orthology more info
enables flavin adenine dinucleotide binding	IDA Inferred from Direct Assay more info	PubMed
enables heme binding	IBA Inferred from Biological aspect of Ancestor more info
enables heme binding	IDA Inferred from Direct Assay more info	PubMed
enables heme binding	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in NADP metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in cell development	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cell development	ISO Inferred from Sequence Orthology more info
involved_in glucose homeostasis	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within glucose homeostasis	ISO Inferred from Sequence Orthology more info
involved_in insulin secretion	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within insulin secretion	ISO Inferred from Sequence Orthology more info
involved_in reactive oxygen species metabolic process	ISO Inferred from Sequence Orthology more info
involved_in response to antibiotic	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within response to antibiotic	ISO Inferred from Sequence Orthology more info
involved_in superoxide metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in superoxide metabolic process	IEA Inferred from Electronic Annotation more info
involved_in superoxide metabolic process	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
is_active_in endoplasmic reticulum	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
located_in perinuclear region of cytoplasm	IEA Inferred from Electronic Annotation more info
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: cytochrome b5 reductase 4

Names: N-terminal cytochrome b5 and cytochrome b5 oxidoreductase domain-containing protein; NADPH cytochrome B5 oxidoreductase; flavohemoprotein b5+b5R; flavohemoprotein b5/b5R

NP_596918.3

EC 1.6.2.2

XP_006243534.1

EC 1.6.2.2

XP_017450917.1

EC 1.6.2.2

XP_038936668.1

EC 1.6.2.2

XP_063120880.1

EC 1.6.2.2

XP_063120881.1

EC 1.6.2.2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_133427.3 → NP_596918.3 cytochrome b5 reductase 4

See identical proteins and their annotated locations for NP_596918.3

Status: VALIDATED

Source sequence(s)

BC080240, JAXUCZ010000008

UniProtKB/Swiss-Prot

Q68EJ0, Q6VXY2, Q6VXY3, Q9EPZ4

UniProtKB/TrEMBL

A0A0G2JSK2, A6I1W7

Related

ENSRNOP00000013909.4, ENSRNOT00000013908.7

Conserved Domains (3) summary

cd06183
Location:277 → 518

cyt_b5_reduct_like; Cytochrome b5 reductase catalyzes the reduction of 2 molecules of cytochrome b5 using NADH as an electron donor. Like ferredoxin reductases, these proteins have an N-terminal FAD binding subdomain and a C-terminal NADH binding subdomain, separated by a ...

cd06490
Location:169 → 255

p23_NCB5OR; p23_like domain found in NAD(P)H cytochrome b5 (NCB5) oxidoreductase (OR) and similar proteins. NCB5OR is widely expressed in human organs and tissues and is localized in the ER (endoplasmic reticulum). It appears to play a critical role in maintaining ...

pfam00173
Location:56 → 128

Cyt-b5; Cytochrome b5-like Heme/Steroid binding domain

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086026.1 Reference GRCr8

Range

96860415..96925789

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039080740.2 → XP_038936668.1 cytochrome b5 reductase 4 isoform X2

UniProtKB/TrEMBL

A6I1W6

Related

ENSRNOP00000111146.1, ENSRNOT00000162833.1

Conserved Domains (3) summary

cd06183
Location:243 → 484

cyt_b5_reduct_like; Cytochrome b5 reductase catalyzes the reduction of 2 molecules of cytochrome b5 using NADH as an electron donor. Like ferredoxin reductases, these proteins have an N-terminal FAD binding subdomain and a C-terminal NADH binding subdomain, separated by a ...

cd06490
Location:135 → 221

p23_NCB5OR; p23_like domain found in NAD(P)H cytochrome b5 (NCB5) oxidoreductase (OR) and similar proteins. NCB5OR is widely expressed in human organs and tissues and is localized in the ER (endoplasmic reticulum). It appears to play a critical role in maintaining ...

pfam00173
Location:24 → 96

Cyt-b5; Cytochrome b5-like Heme/Steroid binding domain
XM_063264810.1 → XP_063120880.1 cytochrome b5 reductase 4 isoform X4
XM_017595428.3 → XP_017450917.1 cytochrome b5 reductase 4 isoform X3

UniProtKB/Swiss-Prot

Q68EJ0, Q6VXY2, Q6VXY3, Q9EPZ4

UniProtKB/TrEMBL

A0A8I6GKQ4

Related

ENSRNOP00000093571.1, ENSRNOT00000111365.2
XM_006243472.5 → XP_006243534.1 cytochrome b5 reductase 4 isoform X1

UniProtKB/Swiss-Prot

Q68EJ0, Q6VXY2, Q6VXY3, Q9EPZ4

UniProtKB/TrEMBL

A0A8I6A4B1

Related

ENSRNOP00000085891.1, ENSRNOT00000119185.2

Conserved Domains (3) summary

cd06183
Location:267 → 508

cyt_b5_reduct_like; Cytochrome b5 reductase catalyzes the reduction of 2 molecules of cytochrome b5 using NADH as an electron donor. Like ferredoxin reductases, these proteins have an N-terminal FAD binding subdomain and a C-terminal NADH binding subdomain, separated by a ...

cd06490
Location:159 → 245

p23_NCB5OR; p23_like domain found in NAD(P)H cytochrome b5 (NCB5) oxidoreductase (OR) and similar proteins. NCB5OR is widely expressed in human organs and tissues and is localized in the ER (endoplasmic reticulum). It appears to play a critical role in maintaining ...

pfam00173
Location:56 → 128

Cyt-b5; Cytochrome b5-like Heme/Steroid binding domain
XM_063264811.1 → XP_063120881.1 cytochrome b5 reductase 4 isoform X5