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    Prkg2 protein kinase cGMP-dependent 2 [ Rattus norvegicus (Norway rat) ]

    Gene ID: 25523, updated on 27-Nov-2024

    Summary

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    Official Symbol
    Prkg2provided by RGD
    Official Full Name
    protein kinase cGMP-dependent 2provided by RGD
    Primary source
    RGD:3401
    See related
    EnsemblRapid:ENSRNOG00000002361 AllianceGenome:RGD:3401
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Rattus norvegicus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
    Also known as
    cGKII; GDPKII
    Summary
    Enables cGMP-dependent protein kinase activity and identical protein binding activity. Involved in several processes, including negative regulation of chloride transport; peptidyl-serine autophosphorylation; and positive regulation of chondrocyte differentiation. Located in nuclear membrane. Biomarker of withdrawal disorder. Human ortholog(s) of this gene implicated in acromesomelic dysplasia-4 and spondylometaphyseal dysplasia. Orthologous to human PRKG2 (protein kinase cGMP-dependent 2). [provided by Alliance of Genome Resources, Nov 2024]
    Expression
    Biased expression in Adrenal (RPKM 73.7), Brain (RPKM 40.7) and 4 other tissues See more
    Orthologs
    human mouse all
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    Genomic context

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    See Prkg2 in Genome Data Viewer
    Location:
    14p22
    Exon count:
    19
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCr8 (GCF_036323735.1) 14 NC_086032.1 (10864020..10972617)
    RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) 14 NC_051349.1 (10559882..10668479)
    106 previous assembly Rnor_6.0 (GCF_000001895.5) 14 NC_005113.4 (12217121..12313616)

    Chromosome 14 - NC_086032.1Genomic Context describing neighboring genes Neighboring gene RasGEF domain family, member 1B Neighboring gene uncharacterized LOC120096570 Neighboring gene PRELI domain containing 1, pseudogene 4 Neighboring gene uncharacterized LOC134481716 Neighboring gene bone morphogenetic protein 3 Neighboring gene cilia and flagella associated protein 299 Neighboring gene small nucleolar RNA SNORA17

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
    • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
    • BioProject: PRJNA238328
    • Publication: PMID 24510058
    • Analysis date: Mon Jun 6 17:44:12 2016

    Bibliography

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    Related articles in PubMed

    1. The regulation of synaptic vesicle recycling by cGMP-dependent protein kinase type II in cerebellar granule cells under strong and sustained stimulation. Collado-Alsina A, et al. J Neurosci, 2014 Jun 25. PMID 24966379, Free PMC Article
    2. Phenotypic characterization of the Komeda miniature rat Ishikawa, an animal model of dwarfism caused by a mutation in Prkg2. Tsuchida A, et al. Comp Med, 2008 Dec. PMID 19149413, Free PMC Article
    3. cGMP-dependent protein kinase type II regulates basal level of aldosterone production by zona glomerulosa cells without increasing expression of the steroidogenic acute regulatory protein gene. Gambaryan S, et al. J Biol Chem, 2003 Aug 8. PMID 12775716
    4. Protein kinase G type II is required for night-to-day progression of the mammalian circadian clock. Tischkau SA, et al. Neuron, 2004 Aug 19. PMID 15312652
    5. STa and cGMP stimulate CFTR translocation to the surface of villus enterocytes in rat jejunum and is regulated by protein kinase G. Golin-Bisello F, et al. Am J Physiol Cell Physiol, 2005 Sep. PMID 15872007

    See all (27) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Wild-type PKGII phosphorylated Raf-1 at Ser-43 in a cGMP-dependent manner, but a PKGII D412A/R415A mutant, which has a low affinity for cGMP, did not.
      Title: Type II cGMP-dependent protein kinase negatively regulates fibroblast growth factor signaling by phosphorylating Raf-1 at serine 43 in rat chondrosarcoma cells.
    2. We conclude that cGKII controls the homeostatic balance of vesicle exocytosis and endocytosis in synaptic boutons of rat cerebellar granule cells.
      Title: The regulation of synaptic vesicle recycling by cGMP-dependent protein kinase type II in cerebellar granule cells under strong and sustained stimulation.
    3. Which in turn activates cGKII and induces the phosphorylation of GluA1.
      Title: The type II cGMP dependent protein kinase regulates GluA1 levels at the plasma membrane of developing cerebellar granule cells.
    4. Sodium depletion enhances renal expression of (pro)renin receptor via cyclic GMP-protein kinase G signaling pathway.
      Title: Sodium depletion enhances renal expression of (pro)renin receptor via cyclic GMP-protein kinase G signaling pathway.
    5. confirmed mutation in Prkg2 is a valuable model for studying dwarfism and longitudinal growth traits
      Title: Phenotypic characterization of the Komeda miniature rat Ishikawa, an animal model of dwarfism caused by a mutation in Prkg2.
    6. a subset of phosphorylation sites is modulated, in a cGMP-PKG-specific manner, in intact HEK cells heterologously expressing Cav1.2 alpha(1c) and beta(2a) subunits
      Title: Protein kinase G phosphorylates Cav1.2 alpha1c and beta2 subunits.
    7. PKG II activity in zona glomerulosa cells is important for maintaining basal aldosterone production
      Title: cGMP-dependent protein kinase type II regulates basal level of aldosterone production by zona glomerulosa cells without increasing expression of the steroidogenic acute regulatory protein gene.
    8. results show that the fast phase of autophosphorylation of cGMP dependent protein kinase II has a small effect on its activity, whereas the secondary phase involving Ser126 phosphorylation may generate an active form
      Title: Autophosphorylation of cGMP-dependent protein kinase type II.
    9. Protein kinase G regulation of interleukin 6 promoter signaling pathway largely prevented fluid shear stress-induced increases of IL-6 mRNA in osteosarcoma cells.
      Title: Guanosine 3',5'-cyclic monophosphate (cGMP)/cGMP-dependent protein kinase induce interleukin-6 transcription in osteoblasts.
    10. eNOS, nNOS, cGMP and protein kinase G mediate the inhibitory effect of pancreastatin on growth and proliferation of hepatoma cells
      Title: eNOS, nNOS, cGMP and protein kinase G mediate the inhibitory effect of pancreastatin, a chromogranin A-derived peptide, on growth and proliferation of hepatoma cells.
    Submit: New GeneRIF Correction See all GeneRIFs (17)

    Pathways from PubChem

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    General gene information

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    Markers

    Homology

    Gene Ontology Provided by RGD

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables cGMP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables cGMP-dependent protein kinase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables cGMP-dependent protein kinase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables mitogen-activated protein kinase binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables mitogen-activated protein kinase binding ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    enables protein serine kinase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables protein serine kinase activity ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    Process Evidence Code Pubs
    involved_in cellular hypotonic response ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within circadian regulation of gene expression ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within circadian rhythm ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in negative regulation of cell volume ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in negative regulation of chloride transport IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in peptidyl-serine autophosphorylation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in positive regulation of chondrocyte differentiation IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in positive regulation of protein localization IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in protein localization to plasma membrane IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in protein phosphorylation ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in protein phosphorylation TAS
    Traceable Author Statement
    more info
    		 PubMed 
    involved_in signal transduction IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in signal transduction ISO
    Inferred from Sequence Orthology
    more info
    		  
    Component Evidence Code Pubs
    located_in apical plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nuclear membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane ISO
    Inferred from Sequence Orthology
    more info
    		  

    General protein information

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    Preferred Names
    cGMP-dependent protein kinase 2
    Names
    CGK 2
    cGK2
    cGMP dependent protein kinase type II
    cGMP-dependent protein kinase II
    protein kinase, cGMP- dependent, type 2
    protein kinase, cGMP-dependent, type II
    type II cGMP-dependent protein kinase
    NP_037144.1
    XP_038947566.1
    XP_063128946.1
    XP_063128947.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_013012.2 → NP_037144.1  cGMP-dependent protein kinase 2

      See identical proteins and their annotated locations for NP_037144.1

      Status: VALIDATED

      Source sequence(s)
      JAXUCZ010000014
      UniProtKB/Swiss-Prot
      Q64595
      Related
      ENSRNOP00000003237.2, ENSRNOT00000003237.6
      Conserved Domains (4) summary
      PTZ00263
      Location:417 → 752
      PTZ00263; protein kinase A catalytic subunit; Provisional
      COG0664
      Location:162 → 285
      Crp; cAMP-binding domain of CRP or a regulatory subunit of cAMP-dependent protein kinases [Signal transduction mechanisms]
      cd00038
      Location:286 → 401
      CAP_ED; effector domain of the CAP family of transcription factors; members include CAP (or cAMP receptor protein (CRP)), which binds cAMP, FNR (fumarate and nitrate reduction), which uses an iron-sulfur cluster to sense oxygen) and CooA, a heme containing CO ...
      cd05572
      Location:459 → 718
      STKc_cGK; Catalytic domain of the Serine/Threonine Kinase, cGMP-dependent protein kinase (cGK or PKG)

    RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCr8

    Genomic

    1. NC_086032.1 Reference GRCr8

      Range
      10864020..10972617
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_039091638.2 → XP_038947566.1  cGMP-dependent protein kinase 2 isoform X1

      Conserved Domains (2) summary
      cd00038
      Location:286 → 401
      CAP_ED; effector domain of the CAP family of transcription factors; members include CAP (or cAMP receptor protein (CRP)), which binds cAMP, FNR (fumarate and nitrate reduction), which uses an iron-sulfur cluster to sense oxygen) and CooA, a heme containing CO ...
      cd05572
      Location:439 → 689
      STKc_cGK; Catalytic domain of the Serine/Threonine Kinase, cGMP-dependent protein kinase (cGK or PKG)

    2. XM_063272876.1 → XP_063128946.1  cGMP-dependent protein kinase 2 isoform X2

    3. XM_063272877.1 → XP_063128947.1  cGMP-dependent protein kinase 2 isoform X3

    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q64595.1 GenPept UniProtKB/Swiss-Prot:Q64595

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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