KU80 Ku80 family protein [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: KU80
Official Full Name: Ku80 family protein
Primary source: TAIR:AT1G48050
Locus tag: AT1G48050
See related: Araport:AT1G48050
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: ARABIDOPSIS THALIANA KU80 HOMOLOG; ATKU80; KU80-LIKE PROTEIN; T2J15.4
Summary: Ku80 and ku70 form the heterodimer complex Ku, required for proper maintenance of the telomeric C strand. Ku regulates the extension of the telomeric G strand. Interacts with WEX, and this interaction stimulates the WEX exonuclease activity. Binds double stranded DNA breaks as a heterodimer with Ku70, involved in non-homologous end joining repair. Mutants are defective in T-DNA integration. Over expression confers increased resistance to DNA damage agents and increased susceptibility to T-DNA transformation.
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Genomic context

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See KU80 in Genome Data Viewer

Location:: chromosome: 1

Exon count:: 12

Sequence:: Chromosome: 1; NC_003070.9 (17723382..17727243)

Chromosome 1 - NC_003070.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003070.9

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Effects of Arabidopsis Ku80 deletion on the integration of the left border of T-DNA into plant chromosomal DNA via Agrobacterium tumefaciens.
Title: Effects of Arabidopsis Ku80 deletion on the integration of the left border of T-DNA into plant chromosomal DNA via Agrobacterium tumefaciens.
We found that larger deletions of >20 bp predominated after DSB repair in ku80 and ku80 parp1 parp2 mutants, corroborating with a role of KU in preventing DSB end resection. Deletion lengths did not significantly differ between ku80 and ku80 parp1 parp2 mutants, suggesting that a KU- and PARP-independent b-NHEJ mechanism becomes active in these mutants.
Title: CRISPR/Cas9-Induced Double-Strand Break Repair in Arabidopsis Nonhomologous End-Joining Mutants.
The speed of a systemic infection of geminivirus in Arabidopsis thaliana is KU80-dependent.
Title: KU80, a key factor for non-homologous end-joining, retards geminivirus multiplication.
The genome stability of the Arabidopsis thaliana mutants deficient in ATM (communication between DNA strand break recognition and the repair machinery), KU80 (deficient in NHEJ) and RAD51B (deficient in HR repair) genes, was investigated.
Title: Genome stability of Arabidopsis atm, ku80 and rad51b mutants: somatic and transgenerational responses to stress.
role during the first minutes post-irradiation
Title: Xrcc1-dependent and Ku-dependent DNA double-strand break repair kinetics in Arabidopsis plants.
Ku70 and Ku80 expression in 3-week-old young seedlings were down-regulated by heat stress in a time-dependent manner, as examined using real-time quantitative PCR, GUS reporter systems, and western blotting analysis.
Title: Regulation of Arabidopsis thaliana Ku genes at different developmental stages under heat stress.
KU80 bridges between dsT-DNAs and double-strand breaks
Title: Involvement of KU80 in T-DNA integration in plant cells.
Ku70/80 specifically inhibits circle formation at telomeres, but not at centromeric and ribosomal DNA repeats.
Title: Ku suppresses formation of telomeric circles and alternative telomere lengthening in Arabidopsis.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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NM_001333292.1

Supported by ESTs:

NM_001333293.1

Supported by ESTs:

NM_103701.2

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables DNA helicase activity	IEA Inferred from Electronic Annotation more info
enables damaged DNA binding	IEA Inferred from Electronic Annotation more info
enables double-stranded DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables telomeric DNA binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within DNA integration	TAS Traceable Author Statement more info	PubMed
acts_upstream_of_or_within DNA repair	TAS Traceable Author Statement more info	PubMed
acts_upstream_of_or_within double-strand break repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in double-strand break repair via nonhomologous end joining	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within double-strand break repair via nonhomologous end joining	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within double-strand break repair via nonhomologous end joining	TAS Traceable Author Statement more info	PubMed
acts_upstream_of_or_within response to heat	IEP Inferred from Expression Pattern more info	PubMed
involved_in telomere maintenance	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within telomere maintenance	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
part_of Ku70:Ku80 complex	IEA Inferred from Electronic Annotation more info
located_in nucleus	ISM Inferred from Sequence Model more info

General protein information

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Preferred Names: Ku80 family protein

NP_564520.1

KU80; FUNCTIONS IN: double-stranded DNA binding, protein binding; INVOLVED IN: in 6 processes; LOCATED IN: nucleus; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Ku70/Ku80, N-terminal alpha/beta (InterPro:IPR005161), DNA helicase, ATP-dependent, Ku type (InterPro:IPR006164), Spen Paralogue and Orthologue SPOC, C-terminal-like (InterPro:IPR016194), Ku70/Ku80 C-terminal arm (InterPro:IPR005160), Ku, C-terminal (InterPro:IPR014893); Has 786 Blast hits to 750 proteins in 217 species: Archae - 0; Bacteria - 40; Metazoa - 246; Fungi - 345; Plants - 62; Viruses - 0; Other Eukaryotes - 93 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003070.9 Reference assembly

Range

17723382..17727243

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_103701.2 → NP_564520.1 Ku80 family protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_564520.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9C7Z3, Q9FQ09, Q9LNF5

UniProtKB/TrEMBL

A0A5S9WMX7

Conserved Domains (3) summary

cd00873
Location:216 → 517

KU80; Ku-core domain, Ku80 subfamily; Ku80 is a subunit of the Ku protein, which plays a key role in multiple nuclear processes such as DNA repair, chromosome maintenance, transcription regulation, and V(D)J recombination. The mechanism underlying the ...

pfam08785
Location:558 → 672

Ku_PK_bind; Ku C terminal domain like

cl00057
Location:5 → 238

vWFA; Von Willebrand factor type A (vWA) domain was originally found in the blood coagulation protein von Willebrand factor (vWF). Typically, the vWA domain is made up of approximately 200 amino acid residues folded into a classic a/b para-rossmann type of ...
NM_001333292.1 → NP_001322094.1 Ku80 family protein [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/TrEMBL

A0A1P8ASX4, A0A5S9WMX7
NM_001333293.1 → NP_001322093.1 Ku80 family protein [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/TrEMBL

A0A178WJ76, A0A1P8AT14