XT2 UDP-xylosyltransferase 2 [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: XT2
Official Full Name: UDP-xylosyltransferase 2
Primary source: TAIR:AT4G02500
Locus tag: AT4G02500
See related: Araport:AT4G02500
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: ARABIDOPSIS THALIANA UDP-XYLOSYLTRANSFERASE 2; ATXT2; AtXXT2; T10P11.20; UDP-xylosyltransferase 2; XXT2; XYG XYLOSYLTRANSFERASE 2
Summary: Encodes a protein with xylosyltransferase activity, which is specific for UDP-xylose as donor substrate and for oligosaccharides with a degree of polymerization >4. Although the enzyme utilizes either cellopentaose or cellohexaose, its activity is four-fold higher with cellohexaose as an acceptor compared to cellopentaose. The enzyme is able to add several xylosyl residues to the acceptor forming mono-, di- and trixylosylated polysaccharides.
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Genomic context

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See XT2 in Genome Data Viewer

Location:: chromosome: 4

Exon count:: 2

Sequence:: Chromosome: 4; NC_003075.7 (1101378..1103612)

Chromosome 4 - NC_003075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003075.7

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Lack of xyloglucan in the cell walls of the Arabidopsis xxt1/xxt2 mutant results in specific increases in homogalacturonan and glucomannan.
Title: Lack of xyloglucan in the cell walls of the Arabidopsis xxt1/xxt2 mutant results in specific increases in homogalacturonan and glucomannan.
ccording to the XXT2 homology model, six amino acids (Phe204, Lys207, Asp228, Ser229, Asp230, His378) were selected and their contribution in catalytic activity was investigated. Site-directed mutagenesis studies show that Asp228, Asp230 and His378 are critical for XXT2 activity and are predicted to be involved in coordination of manganese ion
Title: A homology model of Xyloglucan Xylosyltransferase 2 reveals critical amino acids involved in substrate binding.
Normal growth was restored in the mur3-3 mutant overexpressing XLT2 and in mur3-3 xxt double mutants when the dysfunctional xyloglucan was modified by doubling the amounts of galactosylated side chains.
Title: Galactose-depleted xyloglucan is dysfunctional and leads to dwarfism in Arabidopsis.
The supramolecular organization of the CSLC4, XXT1, XXT2, and XXT5 proteins in Arabidopsis protoplasts, was investigated.
Title: Xyloglucan xylosyltransferases XXT1, XXT2, and XXT5 and the glucan synthase CSLC4 form Golgi-localized multiprotein complexes.
XXT1 and XXT2 are responsible for the bulk of the xylosylation of the glucan backbone, and at least one of these proteins must be present and active for xyloglucan to be made. XXT5 plays a significant but as yet uncharacterized role in this process.
Title: Mutations in multiple XXT genes of Arabidopsis reveal the complexity of xyloglucan biosynthesis.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

NM_116484.3

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables UDP-xylosyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables glycosyltransferase activity	ISS Inferred from Sequence or Structural Similarity more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein homodimerization activity	IPI Inferred from Physical Interaction more info	PubMed
enables xyloglucan 6-xylosyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables xyloglucan 6-xylosyltransferase activity	IGI Inferred from Genetic Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within polysaccharide biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within root hair elongation	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within xyloglucan biosynthetic process	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within xyloglucan metabolic process	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi apparatus	HDA more info	PubMed
located_in Golgi apparatus	ISM Inferred from Sequence Model more info
located_in Golgi trans cisterna	HDA more info	PubMed
located_in cytosol	HDA more info	PubMed
located_in endosome	HDA more info	PubMed
located_in trans-Golgi network	HDA more info	PubMed

General protein information

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Preferred Names: UDP-xylosyltransferase 2

NP_567241.1

UDP-xylosyltransferase 2 (XT2); FUNCTIONS IN: xyloglucan 6-xylosyltransferase activity, UDP-xylosyltransferase activity, transferase activity, transferring glycosyl groups, transferase activity; INVOLVED IN: polysaccharide biosynthetic process, xyloglucan biosynthetic process, root hair elongation; LOCATED IN: mitochondrion, integral to membrane; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Galactosyl transferase (InterPro:IPR008630); BEST Arabidopsis thaliana protein match is: xylosyltransferase 1 (TAIR:AT3G62720.2); Has 461 Blast hits to 460 proteins in 94 species: Archae - 0; Bacteria - 6; Metazoa - 0; Fungi - 152; Plants - 284; Viruses - 0; Other Eukaryotes - 19 (source: NCBI BLink).