Ebf2 early B cell factor 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ebf2provided by MGI
Official Full Name: early B cell factor 2provided by MGI
Primary source: MGI:MGI:894332
See related: Ensembl:ENSMUSG00000022053 AllianceGenome:MGI:894332
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: OE-3; EBF-2; Mmot1; O/E-3; D14Ggc1e
Summary: Enables DNA-binding transcription activator activity, RNA polymerase II-specific; RNA polymerase II cis-regulatory region sequence-specific DNA binding activity; and chromatin binding activity. Involved in positive regulation of cold-induced thermogenesis and positive regulation of transcription by RNA polymerase II. Acts upstream of or within several processes, including adipose tissue development; brown fat cell differentiation; and positive regulation of DNA-templated transcription. Predicted to be located in nucleoplasm. Is expressed in several structures, including branchial arch; central nervous system; embryo mesenchyme; genitourinary system; and sensory organ. Orthologous to human EBF2 (EBF transcription factor 2). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in limb E14.5 (RPKM 7.4), CNS E11.5 (RPKM 7.0) and 10 other tissues See more
Orthologs: human all
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Genomic context

Location:: 14 D1; 14 34.63 cM

Exon count:: 17

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	14	NC_000080.7 (67470741..67668401)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	14	NC_000080.6 (67233292..67430952)

Chromosome 14 - NC_000080.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Age-Progressive and Gender-Dependent Bone Phenotype in Mice Lacking Both Ebf1 and Ebf2 in Prrx1-Expressing Mesenchymal Cells.
Title: Age-Progressive and Gender-Dependent Bone Phenotype in Mice Lacking Both Ebf1 and Ebf2 in Prrx1-Expressing Mesenchymal Cells.
ZFP423 controls EBF2 coactivator recruitment and PPARgamma occupancy to determine the thermogenic plasticity of adipocytes.
Title: ZFP423 controls EBF2 coactivator recruitment and PPARγ occupancy to determine the thermogenic plasticity of adipocytes.
Early B Cell Factor Activity Controls Developmental and Adaptive Thermogenic Gene Programming in Adipocytes.
Title: Early B Cell Factor Activity Controls Developmental and Adaptive Thermogenic Gene Programming in Adipocytes.
Using genetic fate mapping, it was determined that Ebf2 mRNA is initially found in all Purkinje cell progenitors during cerebellar development.
Title: Dynamic Expression and New Functions of Early B Cell Factor 2 in Cerebellar Development.
Down-regulation of EBF2 (and EBF1) genes leads to severe impairment of myelin formation in Schwann cells.
Title: The Transcription Factors EBF1 and EBF2 Are Positive Regulators of Myelination in Schwann Cells.
we show that SIX1 binds to adipogenic and brown marker genes and interacts with C/EBPa, C/EBPb and EBF2, suggesting their functional cooperation during adipogenesis.
Title: Comparative Transcriptomic and Epigenomic Analyses Reveal New Regulators of Murine Brown Adipogenesis.
Data indicate that Contactin-1 expression is dependent upon EBF factors; Cntn1 gene belongs to the expanding regulatory cascade driven by these transcriptional regulators so that changes in its activation may contribute to the Ebf2 null mutant phenotype
Title: The gene encoding the mouse contactin-1 axonal glycoprotein is regulated by the collier/Olf1/EBF family early B-Cell factor 2 transcription factor.
Study identified Ebf2 as a novel and important regulator selectively required for midbrain periaqueductal gray matter dopamine neuron development
Title: Ebf2 is required for development of dopamine neurons in the midbrain periaqueductal gray matter of mouse.
Results indicate that early B cell factor 2 (Ebf2) specifically marks and regulates the molecular profile of brown preadipose cells.
Title: Ebf2 is a selective marker of brown and beige adipogenic precursor cells.
Blnc1 forms a ribonucleoprotein complex with transcription factor EBF2 to stimulate the thermogenic gene program. Further, Blnc1 itself is a target of EBF2, thereby forming a feedforward regulatory loop to drive adipogenesis toward thermogenic phenotype.
Title: A long noncoding RNA transcriptional regulatory circuit drives thermogenic adipocyte differentiation.

Submit: New GeneRIF Correction See all GeneRIFs (21)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Targeted (9) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

UniSTS:498231 (e-PCR)
- UniSTS:498231

Ebf2 (e-PCR), detects polymorphism
- UniSTS:498231

Ebf2 (e-PCR), detects polymorphism
- UniSTS:142943

Ebf2 (e-PCR), detects polymorphism
- UniSTS:545927

RH114388 (e-PCR)
- UniSTS:160908

U92703 (e-PCR)
- UniSTS:160405

PMC134715P3 (e-PCR)
- UniSTS:270745

D14Mit87 (e-PCR), detects polymorphism
- UniSTS:127444

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription activator activity, RNA polymerase II-specific	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables chromatin binding	IDA Inferred from Direct Assay more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within adipose tissue development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within brown fat cell differentiation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within cell fate determination	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of chromatin binding	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of cold-induced thermogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
located_in nucleoplasm	TAS Traceable Author Statement more info

General protein information

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Preferred Names: transcription factor COE2

Names: MET-mesencephalon-olfactory TF1; MET-mesencephalon-olfactory transcription factor 1; Olf-1/EBF-like 3; metencephalon-mesencephalon-olfactory transcription factor 1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001276387.1 → NP_001263316.1 transcription factor COE2

See identical proteins and their annotated locations for NP_001263316.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same protein.

Source sequence(s)

AK053013, AK139262, BB669324, CT010493

Consensus CDS

CCDS27228.1

UniProtKB/Swiss-Prot

O08792, Q543D5

Related

ENSMUSP00000135500.2, ENSMUST00000176161.8

Conserved Domains (3) summary

cd01175
Location:253 → 337

IPT_COE; IPT domain of the COE family (Col/Olf-1/EBF) of non-basic, helix-loop-helix (HLH)-containing transcription factors. COE family proteins are all transcription factors and play an important role in variety of developmental processes. Mouse EBF is involved ...

pfam16422
Location:20 → 246

COE1_DBD; Transcription factor COE1 DNA-binding domain

pfam16423
Location:338 → 381

COE1_HLH; Transcription factor COE1 helix-loop-helix domain
NM_001360202.1 → NP_001347131.1 transcription factor COE2

Status: VALIDATED

Description

Transcript Variant: This variant (3) differs in 5' UTR, compared to variant 1. Variants 1, 2 and 3 encode the same protein.

Source sequence(s)

BC050922, BM932499, CT010493

Consensus CDS

CCDS27228.1

UniProtKB/Swiss-Prot

O08792, Q543D5

Related

ENSMUSP00000135782.2, ENSMUST00000176029.2

Conserved Domains (3) summary

cd01175
Location:253 → 337

IPT_COE; IPT domain of the COE family (Col/Olf-1/EBF) of non-basic, helix-loop-helix (HLH)-containing transcription factors. COE family proteins are all transcription factors and play an important role in variety of developmental processes. Mouse EBF is involved ...

pfam16422
Location:20 → 246

COE1_DBD; Transcription factor COE1 DNA-binding domain

pfam16423
Location:338 → 381

COE1_HLH; Transcription factor COE1 helix-loop-helix domain
NM_010095.6 → NP_034225.1 transcription factor COE2

See identical proteins and their annotated locations for NP_034225.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses an alternate splice site in 5' UTR, compared to variant 1. Variants 1, 2 and 3 encode the same protein.

Source sequence(s)

AK053013, AK139262, BB669324, CT010493

Consensus CDS

CCDS27228.1

UniProtKB/Swiss-Prot

O08792, Q543D5

Related

ENSMUSP00000022637.8, ENSMUST00000022637.14

Conserved Domains (3) summary

cd01175
Location:253 → 337

IPT_COE; IPT domain of the COE family (Col/Olf-1/EBF) of non-basic, helix-loop-helix (HLH)-containing transcription factors. COE family proteins are all transcription factors and play an important role in variety of developmental processes. Mouse EBF is involved ...

pfam16422
Location:20 → 246

COE1_DBD; Transcription factor COE1 DNA-binding domain

pfam16423
Location:338 → 381

COE1_HLH; Transcription factor COE1 helix-loop-helix domain