Nucb2 nucleobindin 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Nucb2provided by MGI
Official Full Name: nucleobindin 2provided by MGI
Primary source: MGI:MGI:1858179
See related: Ensembl:ENSMUSG00000030659 AllianceGenome:MGI:1858179
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Nefa; Calnuc; Nesfatin-1
Summary: Enables calcium ion binding activity. Predicted to be involved in several processes, including negative regulation of appetite; negative regulation of cell communication; and positive regulation of testosterone secretion. Predicted to act upstream of or within intracellular calcium ion homeostasis. Located in endoplasmic reticulum and nuclear outer membrane. Is expressed in several structures, including extraembryonic component; female reproductive system; and heart. Orthologous to human NUCB2 (nucleobindin 2). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Broad expression in genital fat pad adult (RPKM 8.5), bladder adult (RPKM 5.1) and 20 other tissues See more
Orthologs: human all
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Genomic context

Location:: 7 F1; 7 61.79 cM

Exon count:: 16

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	7	NC_000073.7 (116103579..116139823)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	7	NC_000073.6 (116504352..116540588)

Chromosome 7 - NC_000073.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Nesfatin-1 stimulates BMP5 expression and osteoclastogenesis in rheumatoid arthritis.
Title: Nesfatin-1 stimulates BMP5 expression and osteoclastogenesis in rheumatoid arthritis.
Nesfatin-1 regulates steroidogenesis in mouse Leydig cells.
Title: Nesfatin-1 regulates steroidogenesis in mouse Leydig cells.
Nesfatin-1, a novel energy-regulating peptide, alleviates pulmonary fibrosis by blocking TGF-beta1/Smad pathway in an AMPKalpha-dependent manner.
Title: Nesfatin-1, a novel energy-regulating peptide, alleviates pulmonary fibrosis by blocking TGF-β1/Smad pathway in an AMPKα-dependent manner.
Does NUCB2/Nesfatin-1 Influence Eating Behaviors in Obese Patients with Binge Eating Disorder? Toward a Neurobiological Pathway.
Title: Does NUCB2/Nesfatin-1 Influence Eating Behaviors in Obese Patients with Binge Eating Disorder? Toward a Neurobiological Pathway.
The protective effects of nesfatin-1 in neurological dysfunction after spinal cord injury by inhibiting neuroinflammation.
Title: The protective effects of nesfatin-1 in neurological dysfunction after spinal cord injury by inhibiting neuroinflammation.
Nesfatin-1 alleviated lipopolysaccharide-induced acute lung injury through regulating inflammatory response associated with macrophages modulation.
Title: Nesfatin-1 alleviated lipopolysaccharide-induced acute lung injury through regulating inflammatory response associated with macrophages modulation.
NUCB2/Nesfatin-1 Reduces Obesogenic Diet Induced Inflammation in Mice Subcutaneous White Adipose Tissue.
Title: NUCB2/Nesfatin-1 Reduces Obesogenic Diet Induced Inflammation in Mice Subcutaneous White Adipose Tissue.
Nucleobindin-derived nesfatin-1 and nesfatin-1-like peptide stimulate pro-opiomelanocortin synthesis in murine AtT-20 corticotrophs through the cAMP/PKA/CREB signaling pathway.
Title: Nucleobindin-derived nesfatin-1 and nesfatin-1-like peptide stimulate pro-opiomelanocortin synthesis in murine AtT-20 corticotrophs through the cAMP/PKA/CREB signaling pathway.
Loss of Nucleobindin-2/Nesfatin-1 increases lipopolysaccharide-induced murine acute lung inflammation.
Title: Loss of Nucleobindin-2/Nesfatin-1 increases lipopolysaccharide-induced murine acute lung inflammation.
Central nesfatin-1 activates lipid mobilization in adipose tissue and fatty acid oxidation in muscle via the sympathetic nervous system.
Title: Central nesfatin-1 activates lipid mobilization in adipose tissue and fatty acid oxidation in muscle via the sympathetic nervous system.

Submit: New GeneRIF Correction See all GeneRIFs (46)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3)
Gene trapped (1)
Targeted (2) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables G-protein alpha-subunit binding	ISO Inferred from Sequence Orthology more info
enables G-protein alpha-subunit binding	ISS Inferred from Sequence or Structural Similarity more info
enables calcium ion binding	IBA Inferred from Biological aspect of Ancestor more info
enables calcium ion binding	IDA Inferred from Direct Assay more info	PubMed
enables guanyl-nucleotide exchange factor activity	ISO Inferred from Sequence Orthology more info
enables guanyl-nucleotide exchange factor activity	ISS Inferred from Sequence or Structural Similarity more info
enables tumor necrosis factor receptor binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in insulin metabolic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within intracellular calcium ion homeostasis	TAS Traceable Author Statement more info	PubMed
involved_in negative regulation of appetite	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cAMP-mediated signaling	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of fat cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of glucagon secretion	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of insulin receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of fatty acid oxidation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of testosterone secretion	ISO Inferred from Sequence Orthology more info
involved_in sequestering of metal ion	ISO Inferred from Sequence Orthology more info
involved_in small GTPase-mediated signal transduction	ISO Inferred from Sequence Orthology more info
involved_in small GTPase-mediated signal transduction	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
located_in Golgi apparatus	IEA Inferred from Electronic Annotation more info
located_in Golgi apparatus	ISO Inferred from Sequence Orthology more info
located_in Golgi medial cisterna	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
is_active_in endoplasmic reticulum-Golgi intermediate compartment	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum-Golgi intermediate compartment	ISO Inferred from Sequence Orthology more info
located_in extracellular region	IEA Inferred from Electronic Annotation more info
located_in extracellular space	ISO Inferred from Sequence Orthology more info
located_in nuclear outer membrane	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in perikaryon	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: nucleobindin-2

Names: DNA-binding protein NEFA; prepronefastin; prepronesfatin

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001130479.2 → NP_001123951.1 nucleobindin-2 precursor

See identical proteins and their annotated locations for NP_001123951.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 2. Variants 1 and 2 both encode the same protein.

Source sequence(s)

AC163222, AK152672, AK160442, BF462404, BY142521

Consensus CDS

CCDS52372.1

UniProtKB/Swiss-Prot

P81117

UniProtKB/TrEMBL

Q3UKN6

Related

ENSMUSP00000032895.8, ENSMUST00000032895.15

Conserved Domains (2) summary

PRK12704
Location:343 → 400

PRK12704; phosphodiesterase; Provisional

pfam13499
Location:247 → 322

EF-hand_7; EF-hand domain pair
NM_001360375.1 → NP_001347304.1 nucleobindin-2 precursor

Status: VALIDATED

Description

Transcript Variant: This variant (2) represents the longer transcript. Variants 1 and 2 both encode the same protein.

Source sequence(s)

AC163222

Consensus CDS

CCDS52372.1

UniProtKB/Swiss-Prot

P81117

UniProtKB/TrEMBL

Q3UKN6

Conserved Domains (2) summary

PRK12704
Location:343 → 400

PRK12704; phosphodiesterase; Provisional

pfam13499
Location:247 → 322

EF-hand_7; EF-hand domain pair

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000073.7 Reference GRCm39 C57BL/6J

Range

116103579..116139823

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006508025.4 → XP_006508088.1 nucleobindin-2 isoform X1

See identical proteins and their annotated locations for XP_006508088.1

UniProtKB/Swiss-Prot

P81117

UniProtKB/TrEMBL

Q3UKN6

Conserved Domains (2) summary

PRK12704
Location:343 → 400

PRK12704; phosphodiesterase; Provisional

pfam13499
Location:247 → 322

EF-hand_7; EF-hand domain pair

RNA

XR_004934144.1 RNA Sequence

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

NM_016773.3: Suppressed sequence

Description

NM_016773.3: This RefSeq was permanently suppressed because currently there is insufficient support for the transcript, which appears to retain intronic sequence at its 3' end.