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    Engase endo-beta-N-acetylglucosaminidase [ Mus musculus (house mouse) ]

    Gene ID: 217364, updated on 9-Dec-2024

    Summary

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    Official Symbol
    Engaseprovided by MGI
    Official Full Name
    endo-beta-N-acetylglucosaminidaseprovided by MGI
    Primary source
    MGI:MGI:2443788
    See related
    Ensembl:ENSMUSG00000033857 AllianceGenome:MGI:2443788
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    C130099A03; D230014K01Rik
    Summary
    Predicted to enable mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity. Acts upstream of or within protein deglycosylation. Predicted to be located in cytosol. Orthologous to human ENGASE (endo-beta-N-acetylglucosaminidase). [provided by Alliance of Genome Resources, Dec 2024]
    Expression
    Ubiquitous expression in colon adult (RPKM 10.6), thymus adult (RPKM 10.2) and 28 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Engase in Genome Data Viewer
    Location:
    11 E2; 11 83.21 cM
    Exon count:
    14
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 11 NC_000077.7 (118367635..118380038)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 11 NC_000077.6 (118476802..118489209)

    Chromosome 11 - NC_000077.7Genomic Context describing neighboring genes Neighboring gene C1q and tumor necrosis factor related protein 1 Neighboring gene STARR-seq mESC enhancer starr_31303 Neighboring gene STARR-positive B cell enhancer ABC_E3042 Neighboring gene predicted gene 11747 Neighboring gene ribosomal protein L18 pseudogene Neighboring gene RNA binding protein, fox-1 homolog (C. elegans) 3 Neighboring gene predicted gene, 53655 Neighboring gene predicted gene, 53656

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Non-lysosomal Degradation of Singly Phosphorylated Oligosaccharides Initiated by the Action of a Cytosolic Endo-β-N-acetylglucosaminidase. Harada Y, et al. J Biol Chem, 2016 Apr 8. PMID 26858256, Free PMC Article
    2. Cytosolic N-GlcNAc proteins are formed by the action of endo-β-N-acetylglucosaminidase. Maynard JC, et al. Biochem Biophys Res Commun, 2020 Oct 1. PMID 32782141, Free PMC Article
    3. Lethality of mice bearing a knockout of the Ngly1-gene is partially rescued by the additional deletion of the Engase gene. Fujihira H, et al. PLoS Genet, 2017 Apr. PMID 28426790, Free PMC Article
    4. Liver-specific deletion of Ngly1 causes abnormal nuclear morphology and lipid metabolism under food stress. Fujihira H, et al. Biochim Biophys Acta Mol Basis Dis, 2020 Mar 1. PMID 31733337
    5. Endo-β-N-acetylglucosaminidase forms N-GlcNAc protein aggregates during ER-associated degradation in Ngly1-defective cells. Huang C, et al. Proc Natl Acad Sci U S A, 2015 Feb 3. PMID 25605922, Free PMC Article

    See all (19) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Cytosolic N-GlcNAc proteins are formed by the action of endo-beta-N-acetylglucosaminidase.
      Title: Cytosolic N-GlcNAc proteins are formed by the action of endo-β-N-acetylglucosaminidase.
    2. These observations strongly suggest that the Ngly1- or Ngly1/Engase-deficient mice could serve as a valuable animal model for studies related to the pathogenesis of the NGLY1-deficiency, and that cytoplasmic ENGase represents one of the potential therapeutic targets for this genetic disorder.
      Title: Lethality of mice bearing a knockout of the Ngly1-gene is partially rescued by the additional deletion of the Engase gene.
    3. ENGase efficiently hydrolyzes phosphorylated oligosaccharides that are larger than Man4GlcNAc2-P, generating GlcNAc-1-P and neutral Gn1-type free oligosaccharides.
      Title: Non-lysosomal Degradation of Singly Phosphorylated Oligosaccharides Initiated by the Action of a Cytosolic Endo-β-N-acetylglucosaminidase.
    Submit: New GeneRIF Correction

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Endonuclease-mediated (3) 
    • Targeted (1)  1 citation

    Pathways from PubChem

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    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    Process Evidence Code Pubs
    involved_in N-glycan processing IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within protein deglycosylation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytosol IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    cytosolic endo-beta-N-acetylglucosaminidase
    NP_001346297.1
    NP_001390767.1
    NP_001390768.1
    NP_001390769.1
    NP_001390770.1
    NP_766161.1
    XP_006533194.1
    XP_006533196.1
    XP_030101770.1
    XP_036012510.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001359368.1 → NP_001346297.1  cytosolic endo-beta-N-acetylglucosaminidase isoform 2

      Status: VALIDATED

      Source sequence(s)
      AK048667, BC060640, BY070749
      Conserved Domains (1) summary
      cd06547
      Location:27 → 337
      GH85_ENGase; Endo-beta-N-acetylglucosaminidase (ENGase) hydrolyzes the N-N'-diacetylchitobiosyl core of N-glycosylproteins. The beta-1,4-glycosyl bond located between two N-acetylglucosamine residues is hydrolyzed such that N-acetylglucosamine 1 remains with the ...

    2. NM_001403838.1 → NP_001390767.1  cytosolic endo-beta-N-acetylglucosaminidase isoform 3

      Status: VALIDATED

      Source sequence(s)
      AL591075

    3. NM_001403839.1 → NP_001390768.1  cytosolic endo-beta-N-acetylglucosaminidase isoform 2

      Status: VALIDATED

      Source sequence(s)
      AL591075

    4. NM_001403840.1 → NP_001390769.1  cytosolic endo-beta-N-acetylglucosaminidase isoform 4

      Status: VALIDATED

      Source sequence(s)
      AL591075
      UniProtKB/TrEMBL
      F7CXI4

    5. NM_001403841.1 → NP_001390770.1  cytosolic endo-beta-N-acetylglucosaminidase isoform 5

      Status: VALIDATED

      Source sequence(s)
      AL591075
      UniProtKB/TrEMBL
      F7CXI4

    6. NM_172573.3 → NP_766161.1  cytosolic endo-beta-N-acetylglucosaminidase isoform 1

      See identical proteins and their annotated locations for NP_766161.1

      Status: VALIDATED

      Source sequence(s)
      AK048667, BC060640, BY070749
      Consensus CDS
      CCDS25704.1
      UniProtKB/Swiss-Prot
      Q3TK58, Q8BJF1, Q8BX80
      Related
      ENSMUSP00000117538.3, ENSMUST00000135383.9
      Conserved Domains (1) summary
      cd06547
      Location:118 → 428
      GH85_ENGase; Endo-beta-N-acetylglucosaminidase (ENGase) hydrolyzes the N-N'-diacetylchitobiosyl core of N-glycosylproteins. The beta-1,4-glycosyl bond located between two N-acetylglucosamine residues is hydrolyzed such that N-acetylglucosamine 1 remains with the ...

    RNA

    1. NR_153103.1 RNA Sequence

      Status: VALIDATED

      Source sequence(s)
      AK048667, AK084254, BC060640, BY070749

    2. NR_175777.1 RNA Sequence

      Status: VALIDATED

      Source sequence(s)
      AL591075
      Related
      ENSMUST00000171769.8

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000077.7 Reference GRCm39 C57BL/6J

      Range
      118367635..118380038
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_036156617.1 → XP_036012510.1  cytosolic endo-beta-N-acetylglucosaminidase isoform X3

      Conserved Domains (1) summary
      cd06547
      Location:118 → 428
      GH85_ENGase; Endo-beta-N-acetylglucosaminidase (ENGase) hydrolyzes the N-N'-diacetylchitobiosyl core of N-glycosylproteins. The beta-1,4-glycosyl bond located between two N-acetylglucosamine residues is hydrolyzed such that N-acetylglucosamine 1 remains with the ...

    2. XM_030245910.2 → XP_030101770.1  cytosolic endo-beta-N-acetylglucosaminidase isoform X1

      Conserved Domains (1) summary
      cd06547
      Location:27 → 337
      GH85_ENGase; Endo-beta-N-acetylglucosaminidase (ENGase) hydrolyzes the N-N'-diacetylchitobiosyl core of N-glycosylproteins. The beta-1,4-glycosyl bond located between two N-acetylglucosamine residues is hydrolyzed such that N-acetylglucosamine 1 remains with the ...

    3. XM_006533131.5 → XP_006533194.1  cytosolic endo-beta-N-acetylglucosaminidase isoform X1

      Conserved Domains (1) summary
      cd06547
      Location:27 → 337
      GH85_ENGase; Endo-beta-N-acetylglucosaminidase (ENGase) hydrolyzes the N-N'-diacetylchitobiosyl core of N-glycosylproteins. The beta-1,4-glycosyl bond located between two N-acetylglucosamine residues is hydrolyzed such that N-acetylglucosamine 1 remains with the ...

    4. XM_006533133.4 → XP_006533196.1  cytosolic endo-beta-N-acetylglucosaminidase isoform X5

      UniProtKB/TrEMBL
      F7CXI4
      Related
      ENSMUSP00000125886.2, ENSMUST00000168689.2
      Conserved Domains (1) summary
      cl10447
      Location:3 → 123
      GH18_chitinase-like; The GH18 (glycosyl hydrolase, family 18) type II chitinases hydrolyze chitin, an abundant polymer of beta-1,4-linked N-acetylglucosamine (GlcNAc) which is a major component of the cell wall of fungi and the exoskeleton of arthropods. Chitinases have ...

    RNA

    1. XR_003949406.2 RNA Sequence

    2. XR_004936933.1 RNA Sequence

    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q8BX80.1 GenPept UniProtKB/Swiss-Prot:Q8BX80

    Gene LinkOut

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