Lrat lecithin-retinol acyltransferase (phosphatidylcholine-retinol-O-acyltransferase) [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Lratprovided by MGI
Official Full Name: lecithin-retinol acyltransferase (phosphatidylcholine-retinol-O-acyltransferase)provided by MGI
Primary source: MGI:MGI:1891259
See related: Ensembl:ENSMUSG00000028003 AllianceGenome:MGI:1891259
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: 1300010A18Rik
Summary: Enables phosphatidylcholine-retinol O-acyltransferase activity. Involved in retinol metabolic process. Acts upstream of or within several processes, including cellular response to leukemia inhibitory factor; positive regulation of lipid transport; and vitamin A metabolic process. Located in endoplasmic reticulum. Is expressed in several structures, including extraembryonic component; gut; midbrain; reproductive system; and retina. Used to study Leber congenital amaurosis 14. Human ortholog(s) of this gene implicated in Leber congenital amaurosis 14 and retinitis pigmentosa. Orthologous to human LRAT (lecithin retinol acyltransferase). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in lung adult (RPKM 4.4), ovary adult (RPKM 2.9) and 13 other tissues See more
Orthologs: human all
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Genomic context

Location:: 3 E3; 3 36.81 cM

Exon count:: 2

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	3	NC_000069.7 (82799889..82811281, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	3	NC_000069.6 (82892582..82903974, complement)

Chromosome 3 - NC_000069.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Rod a-wave and cone b-wave amplitudes of electroretinograms were increased in Cntf (-/-) mice, but rod b-wave amplitudes were unchanged compared with those in WT mice. Up-regulated RPE65 and LRAT levels accelerated both the visual cycle rate and recovery rate of rod light sensitivity in Cntf (-/-) mice.
Title: Ciliary neurotrophic factor (CNTF) protects retinal cone and rod photoreceptors by suppressing excessive formation of the visual pigments.
examined LRAT regulatory region is sufficient to achieve strong and selective expression in the eye and testes but not in liver and other organs
Title: Transgenic reporter mice with promoter region of murine LRAT specifically marks lens and meiosis spermatocytes.
LRAT overexpression diminishes intracellular levels of biologically active retinoids and reduces retinoid antitumor efficacy in the murine melanoma
Title: LRAT overexpression diminishes intracellular levels of biologically active retinoids and reduces retinoid antitumor efficacy in the murine melanoma B16F10 cell line.
Mislocalized M-opsin was degraded whereas mislocalized S-opsin accumulated in Lrat(-/-) cones before the onset of massive ventral/central cone degeneration.
Title: Pathophysilogical mechanism and treatment strategies for Leber congenital amaurosis.
Studies provide mechanistic insights into how vitamin A is distributed to peripheral tissues in a regulated manner and identify LRAT as a critical component of this process.
Title: Lecithin:retinol acyltransferase is critical for cellular uptake of vitamin A from serum retinol-binding protein.
Lrat KO mice exhibited increased levels of retinoic acid-responsive genes, including p21.
Title: Diethylnitrosamine-induced hepatocarcinogenesis is suppressed in lecithin:retinol acyltransferase-deficient mice primarily through retinoid actions immediately after carcinogen administration.
Analyses revealed that LRAT undergoes spontaneous, covalent modification upon incubation with a variety of phosphatidylcholine substrates. The addition of an acyl chain occurs at the Cys(161) residue, indicating formation of a thioester intermediate.
Title: An acyl-covalent enzyme intermediate of lecithin:retinol acyltransferase.
FATP1 inhibits 11-cis retinol formation via interaction with the visual cycle retinoid isomerase RPE65 and lecithin:retinol acyltransferase
Title: FATP1 inhibits 11-cis retinol formation via interaction with the visual cycle retinoid isomerase RPE65 and lecithin:retinol acyltransferase.
Data show that overexpression of human LRAT specifically in mice oral basal epithelial cells makes these cells more sensitive to carcinogen induced tumorigenesis.
Title: Overexpression of lecithin:retinol acyltransferase in the epithelial basal layer makes mice more sensitive to oral cavity carcinogenesis induced by a carcinogen.
proximal region together with basal transcription factors may be sufficient to drive Lrat expression.
Title: An essential set of basic DNA response elements is required for receptor-dependent transcription of the lecithin:retinol acyltransferase (Lrat) gene.

Submit: New GeneRIF Correction See all GeneRIFs (18)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (4)
Targeted (7) 1 citation

General gene information

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Markers

Lrat (e-PCR), detects polymorphism
- UniSTS:546848

Lrat (e-PCR), detects polymorphism
- UniSTS:547974

BE197763 (e-PCR)
- UniSTS:207516

AI449251 (e-PCR)
- UniSTS:178852

Lrat (e-PCR), detects polymorphism
- UniSTS:516092

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables O-acyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables O-palmitoyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables phosphatidylcholine-retinol O-acyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables phosphatidylcholine-retinol O-acyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables phosphatidylcholine-retinol O-acyltransferase activity	ISO Inferred from Sequence Orthology more info
enables phosphatidylcholine-retinol O-acyltransferase activity	ISS Inferred from Sequence or Structural Similarity more info
enables retinoic acid binding	IEA Inferred from Electronic Annotation more info
enables retinoic acid binding	ISO Inferred from Sequence Orthology more info
enables retinol binding	IEA Inferred from Electronic Annotation more info
enables retinol binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within 1,2-diacyl-sn-glycero-3-phosphocholine metabolic process	TAS Traceable Author Statement more info	PubMed
acts_upstream_of_or_within cellular response to leukemia inhibitory factor	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within embryo development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in lipid storage	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in maintenance of location in cell	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of lipid transport	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within response to bacterium	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to retinoic acid	IEA Inferred from Electronic Annotation more info
involved_in response to vitamin A	IEA Inferred from Electronic Annotation more info
involved_in retinoid metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in retinol metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in retinol metabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within retinol metabolic process	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within retinol metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in retinol metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in retinol metabolic process	ISO Inferred from Sequence Orthology more info
involved_in retinol metabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in visual perception	IEA Inferred from Electronic Annotation more info
involved_in vitamin A metabolic process	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within vitamin A metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in vitamin A metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
located_in multivesicular body	IEA Inferred from Electronic Annotation more info
located_in multivesicular body	ISO Inferred from Sequence Orthology more info
located_in perinuclear region of cytoplasm	IEA Inferred from Electronic Annotation more info
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in rough endoplasmic reticulum	IBA Inferred from Biological aspect of Ancestor more info
located_in rough endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in rough endoplasmic reticulum	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: lecithin retinol acyltransferase

Names: phosphatidylcholine--retinol O-acyltransferase

NP_076113.1

EC 2.3.1.135

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.