Rchy1 ring finger and CHY zinc finger domain containing 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Rchy1provided by MGI
Official Full Name: ring finger and CHY zinc finger domain containing 1provided by MGI
Primary source: MGI:MGI:1915348
See related: Ensembl:ENSMUSG00000029397 AllianceGenome:MGI:1915348
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: ARNIP; CHIMP; Pirh2; Zfp363; Znf363
Summary: This gene encodes a protein containing CHY-, CTCHY-, and RING-type zinc-fingers. The encoded protein functions as an E3 ubiquitin ligase, and mediates the degradation of target proteins such as p53. The activity of this protein is important in cell cycle regulation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]
Expression: Ubiquitous expression in bladder adult (RPKM 12.9), CNS E14 (RPKM 12.2) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 5 E2; 5 45.58 cM

Exon count:: 11

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	5	NC_000071.7 (92096701..92110988, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	5	NC_000071.6 (91948842..91963145, complement)

Chromosome 5 - NC_000071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Pirh2 modulates the mitochondrial function and cytochrome c-mediated neuronal death during Alzheimer's disease.
Title: Pirh2 modulates the mitochondrial function and cytochrome c-mediated neuronal death during Alzheimer's disease.
Pirh2-dependent DNA damage in neurons induced by the G-quadruplex ligand pyridostatin.
Title: Pirh2-dependent DNA damage in neurons induced by the G-quadruplex ligand pyridostatin.
Pirh2 modulates amyloid-beta aggregation through the regulation of glucose-regulated protein 78 and chaperone-mediated signaling.
Title: Pirh2 modulates amyloid-β aggregation through the regulation of glucose-regulated protein 78 and chaperone-mediated signaling.
These data provide an evidence that RCHY1 negatively regulates HDAC2.
Title: E3 ligase RCHY1 negatively regulates HDAC2.
Findings indicate that PIRH2 has central roles in the ubiquitylation of Chk2 and its turnover and in the regulation of its function.
Title: The E3 ligase PIRH2 polyubiquitylates CHK2 and regulates its turnover.
This study provides evidence that the ubiquitination of p73 mediated by Pirh2 represents an important pathway for controlling the suppressive function of p73
Title: Pirh2, a ubiquitin E3 ligase, inhibits p73 transcriptional activity by promoting its ubiquitination.
these data reveal the in vivo roles of Pirh2 in the regulation of p53 and c-Myc stability and support its role as a tumor suppressor
Title: Role of Pirh2 in mediating the regulation of p53 and c-Myc.
Data show that Pirh2 monoubiquitinates PolH at one of multiple lysine residues, and that monoubiquitination of PolH inhibits its ability to interact with PCNA and bypass UV-induced lesions, leading to decreased viability.
Title: Pirh2 E3 ubiquitin ligase monoubiquitinates DNA polymerase eta to suppress translesion DNA synthesis.
Pirh2 acts as a negative regulator of p27(Kip1) function by promoting ubiquitin-dependent proteasomal degradation
Title: Pirh2 promotes ubiquitin-dependent degradation of the cyclin-dependent kinase inhibitor p27Kip1.
These results are consistent with the hypothesis that increased Pirh2 expression affects lung tumorigenesis by reducing p53 activity.
Title: Expression of Pirh2, a newly identified ubiquitin protein ligase, in lung cancer.

Submit: New GeneRIF Correction See all GeneRIFs (13)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1)
Targeted (1) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D10Mit157.2 (e-PCR), detects polymorphism
- UniSTS:126061

AU042618 (e-PCR)
- UniSTS:159398

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables p53 binding	IEA Inferred from Electronic Annotation more info
enables p53 binding	ISO Inferred from Sequence Orthology more info
enables protein homodimerization activity	IEA Inferred from Electronic Annotation more info
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info
enables signaling receptor binding	ISO Inferred from Sequence Orthology more info
enables ubiquitin protein ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase activity	ISO Inferred from Sequence Orthology more info
enables ubiquitin protein ligase activity	ISS Inferred from Sequence or Structural Similarity more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in positive regulation of proteasomal ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of proteasomal ubiquitin-dependent protein catabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of protein ubiquitination	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein ubiquitination	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein autoubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein autoubiquitination	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein ubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein ubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein ubiquitination	ISS Inferred from Sequence or Structural Similarity more info
involved_in rescue of stalled ribosome	ISO Inferred from Sequence Orthology more info
involved_in rescue of stalled ribosome	ISS Inferred from Sequence or Structural Similarity more info
involved_in ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in ubiquitin-dependent protein catabolic process	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in intracellular membrane-bounded organelle	ISO Inferred from Sequence Orthology more info
located_in nuclear speck	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISS Inferred from Sequence or Structural Similarity more info
part_of ubiquitin ligase complex	ISO Inferred from Sequence Orthology more info
part_of ubiquitin ligase complex	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: RING finger and CHY zinc finger domain-containing protein 1

Names: CH-rich-interacting match with PLAG1; E3 ubiquitin-protein ligase Pirh2; RING-type E3 ubiquitin transferase RCHY1; androgen receptor N-terminal-interacting protein; zinc finger protein 363

NP_001258726.1

EC 2.3.2.27

NP_001408145.1

EC 2.3.2.27

NP_080833.1

EC 2.3.2.27

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001271797.2 → NP_001258726.1 RING finger and CHY zinc finger domain-containing protein 1 isoform 2

See identical proteins and their annotated locations for NP_001258726.1

Status: REVIEWED

Description

Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.

Source sequence(s)

AC165433

Consensus CDS

CCDS71613.1

UniProtKB/TrEMBL

G3UWD8, Q8R593

Related

ENSMUSP00000131270.2, ENSMUST00000169948.2

Conserved Domains (2) summary

pfam13639
Location:104 → 146

zf-RING_2; Ring finger domain

pfam14599
Location:151 → 209

zinc_ribbon_6; Zinc-ribbon
NM_001421216.1 → NP_001408145.1 RING finger and CHY zinc finger domain-containing protein 1 isoform 3

Status: REVIEWED

Source sequence(s)

AC165433
NM_026557.5 → NP_080833.1 RING finger and CHY zinc finger domain-containing protein 1 isoform 1

See identical proteins and their annotated locations for NP_080833.1

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).

Source sequence(s)

AC165433

Consensus CDS

CCDS19424.1

UniProtKB/Swiss-Prot

Q920H0, Q9CR50

UniProtKB/TrEMBL

Q8R593

Related

ENSMUSP00000031345.9, ENSMUST00000031345.15

Conserved Domains (3) summary

pfam05495
Location:20 → 93

zf-CHY; CHY zinc finger

pfam13639
Location:144 → 186

zf-RING_2; Ring finger domain

pfam14599
Location:191 → 249

zinc_ribbon_6; Zinc-ribbon

RNA

NR_073442.1 RNA Sequence

Status: REVIEWED

Description

Transcript Variant: This variant (3) differs in the 5' exon structure, compared to variant 1. This variant is represented as non-coding because it lacks a large portion of the coding region, including the translational start codon, found in variant 1.

Source sequence(s)

AK015870, AK018488, AK078397

Related

ENSMUST00000140670.8