Ptpn12 protein tyrosine phosphatase, non-receptor type 12 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ptpn12provided by MGI
Official Full Name: protein tyrosine phosphatase, non-receptor type 12provided by MGI
Primary source: MGI:MGI:104673
See related: Ensembl:ENSMUSG00000028771 AllianceGenome:MGI:104673
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: PTPG1; P19-PTP; PTP-P19; PTP-PEST
Summary: Enables phosphoprotein phosphatase activity. Acts upstream of or within protein dephosphorylation. Located in cytosol and podosome. Is expressed in several structures, including central nervous system; embryo mesenchyme; extraembryonic component; gut; and retina. Human ortholog(s) of this gene implicated in colorectal cancer. Orthologous to human PTPN12 (protein tyrosine phosphatase non-receptor type 12). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in CNS E14 (RPKM 8.7), CNS E18 (RPKM 8.5) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 5 A3; 5 9.83 cM

Exon count:: 21

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	5	NC_000071.7 (21191643..21261009, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	5	NC_000071.6 (20986645..21055911, complement)

Chromosome 5 - NC_000071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

PTPN12-deficient, ErbB2-dependent breast cancer cells were more resistant to anoikis and had augmented migratory and invasive properties.
Title: Loss of PTPN12 Stimulates Progression of ErbB2-Dependent Breast Cancer by Enhancing Cell Survival, Migration, and Epithelial-to-Mesenchymal Transition.
Integrin beta 8 and PTP-PEST form protein complexes at the leading edge of migrating cells and balance patterns of Rac1 and Cdc42 signaling by controlling the subcellular localization and phosphorylation status of Rho GDP dissociation inhibitor 1.
Title: Protein tyrosine phosphatase-PEST and β8 integrin regulate spatiotemporal patterns of RhoGDI1 activation in migrating cells.
Pyk2 deregulation played a pivotal role in the migration defect caused by PTPN12 deficiency.
Title: Control of dendritic cell migration, T cell-dependent immunity, and autoimmunity by protein tyrosine phosphatase PTPN12 expressed in dendritic cells.
Regulation of the Src kinase-associated phosphoprotein 55 homologue by the protein tyrosine phosphatase PTP-PEST in the control of cell motility.
Title: Regulation of the Src kinase-associated phosphoprotein 55 homologue by the protein tyrosine phosphatase PTP-PEST in the control of cell motility.
macrophage fusion is critically dependent on PTP-PEST.
Title: Macrophage fusion is controlled by the cytoplasmic protein tyrosine phosphatase PTP-PEST/PTPN12.
Overexpression of PTP-PEST substantially inhibits lipopolysaccharide (LPS)-induced tyrosine phosphorylation of IkappaB kinase (IKKbeta) at Tyr188/199 in RAW264.7 cells.
Title: Protein tyrosine phosphatase with proline-glutamine-serine-threonine-rich motifs negatively regulates TLR-triggered innate responses by selectively inhibiting IκB kinase β/NF-κB activation.
PTP-PEST is a key regulator of integrin-mediated functions in endothelial cells.
Title: The phosphatase PTP-PEST/PTPN12 regulates endothelial cell migration and adhesion, but not permeability, and controls vascular development and embryonic viability.
the dephosphorylation of Pyk2 requires dynamin's GTPase activity and is mediated by the tyrosine phosphatase PTP-PEST.
Title: Dynamin and PTP-PEST cooperatively regulate Pyk2 dephosphorylation in osteoclasts.
PTP-PEST controls Pyk2 activity and is a positive regulator of secondary T cell activation.
Title: The phosphatase PTP-PEST promotes secondary T cell responses by dephosphorylating the protein tyrosine kinase Pyk2.
Data show that ERK phosphorylates FAK S910 and recruits PIN1 and PTP-PEST, which colocalize with FAK at the lamellipodia of migrating cells.
Title: FAK phosphorylation by ERK primes ras-induced tyrosine dephosphorylation of FAK mediated by PIN1 and PTP-PEST.

Submit: New GeneRIF Correction See all GeneRIFs (15)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (4)
Gene trapped (1)
Targeted (6) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

BB070754 (e-PCR)
- UniSTS:185615

Ptpn12 (e-PCR), detects polymorphism
- UniSTS:274196

PTPN12_1837 (e-PCR)
- UniSTS:280939

Ptpn12 (e-PCR), detects polymorphism
- UniSTS:536483

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables SH3 domain binding	IEA Inferred from Electronic Annotation more info
enables SH3 domain binding	ISO Inferred from Sequence Orthology more info
enables histone H2AXY142 phosphatase activity	IEA Inferred from Electronic Annotation more info
enables non-membrane spanning protein tyrosine phosphatase activity	IEA Inferred from Electronic Annotation more info
enables phosphoprotein phosphatase activity	IDA Inferred from Direct Assay more info	PubMed
enables phosphoprotein phosphatase activity	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein tyrosine phosphatase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein tyrosine phosphatase activity	ISO Inferred from Sequence Orthology more info
enables protein tyrosine phosphatase activity, metal-dependent	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in cellular response to epidermal growth factor stimulus	ISO Inferred from Sequence Orthology more info
involved_in cellular response to epidermal growth factor stimulus	ISS Inferred from Sequence or Structural Similarity more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in peptidyl-tyrosine dephosphorylation	ISO Inferred from Sequence Orthology more info
involved_in peptidyl-tyrosine dephosphorylation	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within protein dephosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in protein dephosphorylation	ISO Inferred from Sequence Orthology more info
involved_in regulation of epidermal growth factor receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of epidermal growth factor receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in regulation of epidermal growth factor receptor signaling pathway	ISS Inferred from Sequence or Structural Similarity more info
involved_in tissue regeneration	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cell projection	IEA Inferred from Electronic Annotation more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in focal adhesion	IEA Inferred from Electronic Annotation more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in podosome	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: tyrosine-protein phosphatase non-receptor type 12

Names: MPTP-PEST; protein-tyrosine phosphatase P19

NP_001343519.1

EC 3.1.3.48

NP_001343520.1

EC 3.1.3.48

NP_001343521.1

EC 3.1.3.48

NP_001411448.1

EC 3.1.3.48

NP_001411450.1

EC 3.1.3.48

NP_035333.2

EC 3.1.3.48

XP_030110077.1

EC 3.1.3.48

XP_036020804.1

EC 3.1.3.48

XP_036020805.1

EC 3.1.3.48

XP_036020806.1

EC 3.1.3.48

XP_036020807.1

EC 3.1.3.48

XP_036020808.1

EC 3.1.3.48

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001356590.1 → NP_001343519.1 tyrosine-protein phosphatase non-receptor type 12 isoform a

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).

Source sequence(s)

AC116501, AC121572

Conserved Domains (1) summary

cl28904
Location:43 → 312

PTP_DSP_cys; cys-based protein tyrosine phosphatase and dual-specificity phosphatase superfamily
NM_001356591.2 → NP_001343520.1 tyrosine-protein phosphatase non-receptor type 12 isoform c

Status: VALIDATED

Description

Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (c) is shorter at the N-terminus compared to isoform a. Variants 3 and 4 both encode the same isoform (c).

Source sequence(s)

AC116501, AC121572

Conserved Domains (1) summary

cl28904
Location:1 → 179

PTP_DSP_cys; cys-based protein tyrosine phosphatase and dual-specificity phosphatase superfamily
NM_001356592.1 → NP_001343521.1 tyrosine-protein phosphatase non-receptor type 12 isoform c

Status: VALIDATED

Description

Transcript Variant: This variant (4) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (c) is shorter at the N-terminus compared to isoform a. Variants 3 and 4 both encode the same isoform (c).

Source sequence(s)

AC116501, AC121572

Conserved Domains (1) summary

cl28904
Location:1 → 179

PTP_DSP_cys; cys-based protein tyrosine phosphatase and dual-specificity phosphatase superfamily
NM_001424519.1 → NP_001411448.1 tyrosine-protein phosphatase non-receptor type 12 isoform 4

Status: VALIDATED

Source sequence(s)

AC116501, AC121572
NM_001424521.1 → NP_001411450.1 tyrosine-protein phosphatase non-receptor type 12 isoform 5

Status: VALIDATED

Source sequence(s)

AC116501, AC121572
NM_011203.4 → NP_035333.2 tyrosine-protein phosphatase non-receptor type 12 isoform b

See identical proteins and their annotated locations for NP_035333.2

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (b) has a shorter and distinct N-terminus compared to isoform a.

Source sequence(s)

AC116501, AC121572

Consensus CDS

CCDS19102.1

UniProtKB/Swiss-Prot

P35831, Q80UM4

Related

ENSMUSP00000030556.8, ENSMUST00000030556.8

Conserved Domains (2) summary

smart00194
Location:28 → 292

PTPc; Protein tyrosine phosphatase, catalytic domain

cd00047
Location:60 → 292

PTPc; Protein tyrosine phosphatases (PTP) catalyze the dephosphorylation of phosphotyrosine peptides; they regulate phosphotyrosine levels in signal transduction pathways. The depth of the active site cleft renders the enzyme specific for phosphorylated Tyr ...

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000071.7 Reference GRCm39 C57BL/6J

Range

21191643..21261009 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036164914.1 → XP_036020807.1 tyrosine-protein phosphatase non-receptor type 12 isoform X4

Conserved Domains (1) summary

cl28904
Location:1 → 179

PTP_DSP_cys; cys-based protein tyrosine phosphatase and dual-specificity phosphatase superfamily
XM_036164915.1 → XP_036020808.1 tyrosine-protein phosphatase non-receptor type 12 isoform X5

Conserved Domains (1) summary

cl28904
Location:1 → 87

PTP_DSP_cys; cys-based protein tyrosine phosphatase and dual-specificity phosphatase superfamily
XM_036164912.1 → XP_036020805.1 tyrosine-protein phosphatase non-receptor type 12 isoform X2

Conserved Domains (1) summary

cl28904
Location:43 → 312

PTP_DSP_cys; cys-based protein tyrosine phosphatase and dual-specificity phosphatase superfamily
XM_036164911.1 → XP_036020804.1 tyrosine-protein phosphatase non-receptor type 12 isoform X1

Conserved Domains (1) summary

cl28904
Location:43 → 312

PTP_DSP_cys; cys-based protein tyrosine phosphatase and dual-specificity phosphatase superfamily
XM_036164913.1 → XP_036020806.1 tyrosine-protein phosphatase non-receptor type 12 isoform X3

Conserved Domains (1) summary

cl28904
Location:1 → 179

PTP_DSP_cys; cys-based protein tyrosine phosphatase and dual-specificity phosphatase superfamily
XM_030254217.2 → XP_030110077.1 tyrosine-protein phosphatase non-receptor type 12 isoform X3

Conserved Domains (1) summary

cl28904
Location:1 → 179

PTP_DSP_cys; cys-based protein tyrosine phosphatase and dual-specificity phosphatase superfamily