bru1 bruno 1 [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: bru1provided by FlyBase
Official Full Name: bruno 1provided by FlyBase
Primary source: FLYBASE:FBgn0000114
Locus tag: Dmel_CG31762
See related: AllianceGenome:FB:FBgn0000114
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: aret; Aret; ARET; aret/bruno1; arrest/Bruno; BcDNA:GM15173; bru; Bru; bru-1; Bru1; bruno; Bruno; CG31762; CG6319; Dm Bru; Dmel\CG31762; ms(2)01284; NEST:bs01g04; NEST:bs34h11
Summary: Enables mRNA 3'-UTR binding activity. Involved in several processes, including female germ-line stem cell population maintenance; positive regulation of exit from mitosis; and regulation of gene expression. Located in P granule; nuclear body; and nuclear membrane. Is expressed in several structures, including adult head; embryonic anal pad; flight muscle cell; germline cell; and gonad. Human ortholog(s) of this gene implicated in developmental and epileptic encephalopathy 97. Orthologous to human CELF1 (CUGBP Elav-like family member 1) and CELF2 (CUGBP Elav-like family member 2). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 33D2-33E1; 2-46 cM

Exon count:: 20

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2L	NT_033779.5 (12175004..12313438)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2L	NT_033779.4 (12175004..12313438)

Chromosome 2L - NT_033779.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Bruno 1/CELF regulates splicing and cytoskeleton dynamics to ensure correct sarcomere assembly in Drosophila flight muscles.
Title: Bruno 1/CELF regulates splicing and cytoskeleton dynamics to ensure correct sarcomere assembly in Drosophila flight muscles.
Results identify a novel regulatory circuit in drosophila, with Bru restricting the activity of osk, and osk in turn restricting the activity of Bru.
Title: oskar RNA plays multiple noncoding roles to support oogenesis and maintain integrity of the germline/soma distinction.
Here we describe an in vivo assay system to monitor the spatial pattern of Bru-dependent repression, separate from the full complexity of osk regulation.
Title: Region-specific activation of oskar mRNA translation by inhibition of Bruno-mediated repression.
Aret potently promotes flight muscle splicing patterns when ectopically expressed in Drosophila jump muscles.
Title: Arrest is a regulator of fiber-specific alternative splicing in the indirect flight muscles of Drosophila.
A role for Bruno in Enok-controlled GSC maintenance
Title: The Drosophila putative histone acetyltransferase Enok maintains female germline stem cells through regulating Bruno and the niche.
Multiple RNA binding domains of Bruno confer recognition of diverse binding sites for translational repression.(
Title: Multiple RNA binding domains of Bruno confer recognition of diverse binding sites for translational repression.
Bruno RNA recognition motif contains four antiparallel beta-strands and two alpha-helices.
Title: Bruno protein contains an expanded RNA recognition motif.
Repression by Bruno during oogenesis is required to restrict Gcl expression in the early embryo.
Title: Bruno negatively regulates germ cell-less expression in a BRE-independent manner.
Bruno shuttles between the nucleus and cytoplasm, and may first bind oskar mRNA in the nucleus.
Title: Two distinct domains of Bruno bind specifically to the oskar mRNA.
Ectopic expression of bruno prolongs oskar repression and blocks posterior body patterning.
Title: A late phase of Oskar accumulation is crucial for posterior patterning of the Drosophila embryo, and is blocked by ectopic expression of Bruno.

Submit: New GeneRIF Correction See all GeneRIFs (15)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

mobile_element

Loc: 12197314-12197348 mobile_element_type = transposon:INE-1{}2179
Loc: 12262559-12262626 mobile_element_type = transposon:H{}2183
Loc: 12285973-12286003 mobile_element_type = transposon:INE-1{}2181

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables RNA binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables mRNA 3'-UTR binding	IBA Inferred from Biological aspect of Ancestor more info
enables mRNA 3'-UTR binding	IDA Inferred from Direct Assay more info	PubMed
enables mRNA 3'-UTR binding	TAS Traceable Author Statement more info	PubMed
enables mRNA binding	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables mRNA binding	NAS Non-traceable Author Statement more info	PubMed
enables mRNA binding	TAS Traceable Author Statement more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in female germ-line stem cell population maintenance	IGI Inferred from Genetic Interaction more info	PubMed
involved_in germ cell development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mRNA splice site recognition	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of oskar mRNA translation	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of oskar mRNA translation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of oskar mRNA translation	NAS Non-traceable Author Statement more info	PubMed
involved_in negative regulation of oskar mRNA translation	TAS Traceable Author Statement more info	PubMed
involved_in negative regulation of translation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in oogenesis	HMP more info	PubMed
involved_in oogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of exit from mitosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of stem cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of alternative mRNA splicing, via spliceosome	HMP more info	PubMed
involved_in regulation of alternative mRNA splicing, via spliceosome	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of alternative mRNA splicing, via spliceosome	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of myofibril size	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sarcomere organization	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in P granule	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in nuclear body	IDA Inferred from Direct Assay more info	PubMed
located_in nuclear membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IC Inferred by Curator more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IGI Inferred from Genetic Interaction more info	PubMed
part_of ribonucleoprotein complex	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: bruno 1

Names: CG31762-PA; CG31762-PB; CG31762-PD; CG31762-PE; CG31762-PF; CG31762-PG; CG31762-PH; CG31762-PI; CG31762-PJ; CG31762-PK; CG31762-PL; arrest; bru1-PA; bru1-PB; bru1-PD; bru1-PE; bru1-PF; bru1-PG; bru1-PH; bru1-PI; bru1-PJ; bru1-PK; bru1-PL; bruno

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033779.5 Reference assembly

Range

12175004..12313438

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001273478.1 → NP_001260407.1 bruno 1, isoform K [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001260407.1

Status: REVIEWED

UniProtKB/TrEMBL

M9PDB0, O02374

Conserved Domains (4) summary

cd12631
Location:119 → 200

RRM1_CELF1_2_Bruno; RNA recognition motif 1 in CUGBP Elav-like family member CELF-1, CELF-2, Drosophila melanogaster Bruno protein and similar proteins

cd12640
Location:485 → 563

RRM3_Bruno_like; RNA recognition motif 3 in Drosophila melanogaster Bruno protein and similar proteins

TIGR01661
Location:122 → 571

ELAV_HUD_SF; ELAV/HuD family splicing factor

cl17169
Location:206 → 287

RRM_SF; RNA recognition motif (RRM) superfamily
NM_001273477.1 → NP_001260406.1 bruno 1, isoform J [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001260406.1

Status: REVIEWED

UniProtKB/TrEMBL

M9PDB0, O02374

Conserved Domains (4) summary

cd12631
Location:119 → 200

RRM1_CELF1_2_Bruno; RNA recognition motif 1 in CUGBP Elav-like family member CELF-1, CELF-2, Drosophila melanogaster Bruno protein and similar proteins

cd12640
Location:485 → 563

RRM3_Bruno_like; RNA recognition motif 3 in Drosophila melanogaster Bruno protein and similar proteins

TIGR01661
Location:122 → 571

ELAV_HUD_SF; ELAV/HuD family splicing factor

cl17169
Location:206 → 287

RRM_SF; RNA recognition motif (RRM) superfamily
NM_165002.2 → NP_723737.1 bruno 1, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_723737.1

Status: REVIEWED

UniProtKB/TrEMBL

C8VV87, Q8IP90

Related

FBpp0079926

Conserved Domains (4) summary

cd12631
Location:356 → 437

RRM1_CELF1_2_Bruno; RNA recognition motif 1 in CUGBP Elav-like family member CELF-1, CELF-2, Drosophila melanogaster Bruno protein and similar proteins

cd12636
Location:443 → 524

RRM2_Bruno_like; RNA recognition motif 2 in Drosophila melanogaster Bruno protein and similar proteins

cd12640
Location:722 → 800

RRM3_Bruno_like; RNA recognition motif 3 in Drosophila melanogaster Bruno protein and similar proteins

TIGR01661
Location:359 → 808

ELAV_HUD_SF; ELAV/HuD family splicing factor
NM_001414076.1 → NP_001400994.1 bruno 1, isoform L [Drosophila melanogaster]

Status: REVIEWED

UniProtKB/TrEMBL

A0A9F2H0X2
NM_001273475.1 → NP_001260404.1 bruno 1, isoform H [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001260404.1

Status: REVIEWED

UniProtKB/TrEMBL

M9PDB0, O02374

Conserved Domains (4) summary

cd12631
Location:119 → 200

RRM1_CELF1_2_Bruno; RNA recognition motif 1 in CUGBP Elav-like family member CELF-1, CELF-2, Drosophila melanogaster Bruno protein and similar proteins

cd12640
Location:485 → 563

RRM3_Bruno_like; RNA recognition motif 3 in Drosophila melanogaster Bruno protein and similar proteins

TIGR01661
Location:122 → 571

ELAV_HUD_SF; ELAV/HuD family splicing factor

cl17169
Location:206 → 287

RRM_SF; RNA recognition motif (RRM) superfamily
NM_001273476.1 → NP_001260405.1 bruno 1, isoform I [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001260405.1

Status: REVIEWED

UniProtKB/TrEMBL

M9PDB0, O02374

Conserved Domains (4) summary

cd12631
Location:119 → 200

RRM1_CELF1_2_Bruno; RNA recognition motif 1 in CUGBP Elav-like family member CELF-1, CELF-2, Drosophila melanogaster Bruno protein and similar proteins

cd12640
Location:485 → 563

RRM3_Bruno_like; RNA recognition motif 3 in Drosophila melanogaster Bruno protein and similar proteins

TIGR01661
Location:122 → 571

ELAV_HUD_SF; ELAV/HuD family splicing factor

cl17169
Location:206 → 287

RRM_SF; RNA recognition motif (RRM) superfamily
NM_001273474.1 → NP_001260403.1 bruno 1, isoform G [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001260403.1

Status: REVIEWED

UniProtKB/TrEMBL

C8VV87, Q8IP90

Conserved Domains (4) summary

cd12631
Location:356 → 437

RRM1_CELF1_2_Bruno; RNA recognition motif 1 in CUGBP Elav-like family member CELF-1, CELF-2, Drosophila melanogaster Bruno protein and similar proteins

cd12636
Location:443 → 524

RRM2_Bruno_like; RNA recognition motif 2 in Drosophila melanogaster Bruno protein and similar proteins

cd12640
Location:722 → 800

RRM3_Bruno_like; RNA recognition motif 3 in Drosophila melanogaster Bruno protein and similar proteins

TIGR01661
Location:359 → 808

ELAV_HUD_SF; ELAV/HuD family splicing factor
NM_165003.3 → NP_723738.2 bruno 1, isoform F [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_723738.2

Status: REVIEWED

UniProtKB/TrEMBL

O02374, Q8IP89

Conserved Domains (4) summary

cd12631
Location:150 → 231

RRM1_CELF1_2_Bruno; RNA recognition motif 1 in CUGBP Elav-like family member CELF-1, CELF-2, Drosophila melanogaster Bruno protein and similar proteins

cd12640
Location:532 → 610

RRM3_Bruno_like; RNA recognition motif 3 in Drosophila melanogaster Bruno protein and similar proteins

TIGR01661
Location:153 → 618

ELAV_HUD_SF; ELAV/HuD family splicing factor

cl17169
Location:237 → 318

RRM_SF; RNA recognition motif (RRM) superfamily
NM_165004.3 → NP_723739.1 bruno 1, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_723739.1

Status: REVIEWED

UniProtKB/TrEMBL

O02374, Q960Z4

Related

FBpp0079927

Conserved Domains (4) summary

cd12631
Location:150 → 231

RRM1_CELF1_2_Bruno; RNA recognition motif 1 in CUGBP Elav-like family member CELF-1, CELF-2, Drosophila melanogaster Bruno protein and similar proteins

cd12640
Location:516 → 594

RRM3_Bruno_like; RNA recognition motif 3 in Drosophila melanogaster Bruno protein and similar proteins

TIGR01661
Location:153 → 602

ELAV_HUD_SF; ELAV/HuD family splicing factor

cl17169
Location:237 → 318

RRM_SF; RNA recognition motif (RRM) superfamily
NM_001169492.2 → NP_001162963.1 bruno 1, isoform E [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001162963.1

Status: REVIEWED

UniProtKB/TrEMBL

O02374, Q960Z4

Conserved Domains (4) summary

cd12631
Location:150 → 231

RRM1_CELF1_2_Bruno; RNA recognition motif 1 in CUGBP Elav-like family member CELF-1, CELF-2, Drosophila melanogaster Bruno protein and similar proteins

cd12640
Location:516 → 594

RRM3_Bruno_like; RNA recognition motif 3 in Drosophila melanogaster Bruno protein and similar proteins

TIGR01661
Location:153 → 602

ELAV_HUD_SF; ELAV/HuD family splicing factor

cl17169
Location:237 → 318

RRM_SF; RNA recognition motif (RRM) superfamily
NM_165005.3 → NP_723740.1 bruno 1, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_723740.1

Status: REVIEWED

UniProtKB/TrEMBL

G7H844, Q8SYC0

Related

FBpp0079929

Conserved Domains (3) summary

cd12640
Location:273 → 351

RRM3_Bruno_like; RNA recognition motif 3 in Drosophila melanogaster Bruno protein and similar proteins

TIGR01661
Location:2 → 359

ELAV_HUD_SF; ELAV/HuD family splicing factor

cl17169
Location:1 → 75

RRM_SF; RNA recognition motif (RRM) superfamily