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    Washc3 WASH complex subunit 3 [ Mus musculus (house mouse) ]

    Gene ID: 67282, updated on 27-Nov-2024

    Summary

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    Official Symbol
    Washc3provided by MGI
    Official Full Name
    WASH complex subunit 3provided by MGI
    Primary source
    MGI:MGI:1914532
    See related
    Ensembl:ENSMUSG00000020056 AllianceGenome:MGI:1914532
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    Ccdc53; 2900091E11Rik; 5730495F03Rik
    Summary
    Predicted to be involved in actin filament polymerization and exocytosis. Part of WASH complex. Is expressed in several structures, including adipose tissue; central nervous system; eye; liver; and thymus primordium. Orthologous to human WASHC3 (WASH complex subunit 3). [provided by Alliance of Genome Resources, Nov 2024]
    Expression
    Ubiquitous expression in placenta adult (RPKM 9.0), bladder adult (RPKM 8.3) and 28 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Washc3 in Genome Data Viewer
    Location:
    10 C1; 10 43.7 cM
    Exon count:
    12
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 10 NC_000076.7 (88036954..88160001)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 10 NC_000076.6 (88201097..88324139)

    Chromosome 10 - NC_000076.7Genomic Context describing neighboring genes Neighboring gene PARP1 binding protein Neighboring gene predicted gene 6653 Neighboring gene nucleoporin 37 Neighboring gene STARR-seq mESC enhancer starr_27492 Neighboring gene predicted gene, 26357 Neighboring gene STARR-positive B cell enhancer ABC_E6896 Neighboring gene CapStarr-seq enhancer MGSCv37_chr10:87666206-87666315 Neighboring gene predicted gene 8415 Neighboring gene WD repeat domain 75 pseudogene Neighboring gene DNA-damage regulated autophagy modulator 1 Neighboring gene predicted gene, 51779 Neighboring gene STARR-positive B cell enhancer ABC_E2332 Neighboring gene predicted gene, 40733

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. A FAM21-containing WASH complex regulates retromer-dependent sorting. Gomez TS, et al. Dev Cell, 2009 Nov. PMID 19922874, Free PMC Article
    2. Loss of strumpellin in the melanocytic lineage impairs the WASH Complex but does not affect coat colour. Tyrrell BJ, et al. Pigment Cell Melanoma Res, 2016 Sep. PMID 27390154, Free PMC Article
    3. The Arp2/3 activator WASH controls the fission of endosomes through a large multiprotein complex. Derivery E, et al. Dev Cell, 2009 Nov. PMID 19922875
    4. Hdac4 Interactions in Huntington's Disease Viewed Through the Prism of Multiomics. Federspiel JD, et al. Mol Cell Proteomics, 2019 Aug 9. PMID 31040226, Free PMC Article
    5. Construction of long-transcript enriched cDNA libraries from submicrogram amounts of total RNAs by a universal PCR amplification method. Piao Y, et al. Genome Res, 2001 Sep. PMID 11544199, Free PMC Article

    See all (22) citations in PubMed

    GeneRIFs: Gene References Into Functions

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Endonuclease-mediated (1) 
    • Targeted (2) 

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables molecular_function ND
    No biological Data available
    more info
    		  
    Process Evidence Code Pubs
    involved_in actin filament polymerization IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in endosomal transport NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in exocytosis IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in protein transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in regulation of Arp2/3 complex-mediated actin nucleation NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of WASH complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of WASH complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of WASH complex ISO
    Inferred from Sequence Orthology
    more info
    		  
    part_of WASH complex ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    part_of WASH complex NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    located_in early endosome membrane NAS
    Non-traceable Author Statement
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    WASH complex subunit 3
    Names
    WASH complex subunit CCDC53
    coiled-coil domain containing 53
    coiled-coil domain-containing protein 53

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001122960.2 → NP_001116432.1  WASH complex subunit 3 isoform 2

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1, resulting in a shorter protein (isoform 2), compared to isoform 1.
      Source sequence(s)
      AC168315
      Consensus CDS
      CCDS59550.1
      UniProtKB/TrEMBL
      S4R287
      Related
      ENSMUSP00000138540.2, ENSMUST00000182183.8
      Conserved Domains (1) summary
      pfam10152
      Location:30 → 166
      DUF2360; Predicted coiled-coil domain-containing protein (DUF2360)

    2. NM_001429467.1 → NP_001416396.1  WASH complex subunit 3 isoform 4

      Status: VALIDATED

      Source sequence(s)
      AC168315

    3. NM_026070.4 → NP_080346.1  WASH complex subunit 3 isoform 1

      See identical proteins and their annotated locations for NP_080346.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) encodes the longest isoform (1).
      Source sequence(s)
      AC168315
      Consensus CDS
      CCDS24108.1
      UniProtKB/Swiss-Prot
      Q8K2X5, Q9CR27, Q9D0Y7
      Related
      ENSMUSP00000020248.9, ENSMUST00000020248.16
      Conserved Domains (1) summary
      pfam10152
      Location:30 → 167
      DUF2360; Predicted coiled-coil domain-containing protein (DUF2360)

    4. NM_027487.2 → NP_081763.1  WASH complex subunit 3 isoform 3

      See identical proteins and their annotated locations for NP_081763.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (3) contains an alternate exon that creates a unique 3' coding region and 3' UTR, compared to variant 1, resulting in a shorter protein (isoform 3) with a novel C-terminus, compared to isoform 1.
      Source sequence(s)
      AC168315
      Consensus CDS
      CCDS48664.1
      UniProtKB/TrEMBL
      G3UWG7, S4R1P7
      Related
      ENSMUSP00000132246.2, ENSMUST00000171151.9
      Conserved Domains (1) summary
      pfam10152
      Location:30 → 145
      DUF2360; Predicted coiled-coil domain-containing protein (DUF2360)

    RNA

    1. NR_190735.1 RNA Sequence

      Status: VALIDATED

      Source sequence(s)
      AC168315

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000076.7 Reference GRCm39 C57BL/6J

      Range
      88036954..88160001
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_030245232.1 → XP_030101092.1  WASH complex subunit 3 isoform X1

      Related
      ENSMUSP00000138310.2, ENSMUST00000182619.2
      Conserved Domains (1) summary
      pfam10152
      Location:30 → 144
      CCDC53; Subunit CCDC53 of WASH complex

    RNA

    1. XR_003948780.2 RNA Sequence

    2. XR_003948781.2 RNA Sequence

    3. XR_004936257.1 RNA Sequence

    4. XR_003948777.2 RNA Sequence

    5. XR_004936258.1 RNA Sequence

    6. XR_003948779.2 RNA Sequence

    7. XR_003948783.2 RNA Sequence

    8. XR_004936259.1 RNA Sequence

    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9CR27.1 GenPept UniProtKB/Swiss-Prot:Q9CR27

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

    Other Literature Sources
    Chemical Information
    Molecular Biology Databases
    Research Materials
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