Elane elastase, neutrophil expressed [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Elaneprovided by MGI
Official Full Name: elastase, neutrophil expressedprovided by MGI
Primary source: MGI:MGI:2679229
See related: Ensembl:ENSMUSG00000020125 AllianceGenome:MGI:2679229
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: NE; Ela2; F430011M15Rik
Summary: This gene encodes a member of the chymotrypsin-like family of serine protease enzymes that hydrolyzes a broad range of protein substrates including elastin. This gene is expressed by neutrophils where the encoded enzyme is stored in azurophil granules. Upon neutrophil activation, the active enzyme is released into the extracellular mileu. Mice lacking the encoded protein exhibit increased susceptibility to sepsis and death following intraperitoneal infection with Gram negative bacteria. This gene is located adjacent to a related proteinase gene on chromosome 10. [provided by RefSeq, Jul 2016]
Expression: Biased expression in liver E18 (RPKM 61.1), liver E14.5 (RPKM 36.1) and 2 other tissues See more
Orthologs: human all
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Genomic context

Location:: 10 C1; 10 39.72 cM

Exon count:: 5

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	10	NC_000076.7 (79722146..79724050)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	10	NC_000076.6 (79886312..79888216)

Chromosome 10 - NC_000076.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Neutrophil Elastase Remodels Mammary Tumors to Facilitate Lung Metastasis.
Title: Neutrophil Elastase Remodels Mammary Tumors to Facilitate Lung Metastasis.
Causal Role for Neutrophil Elastase in Thoracic Aortic Dissection in Mice.
Title: Causal Role for Neutrophil Elastase in Thoracic Aortic Dissection in Mice.
Degradation of EGFR on lung epithelial cells by neutrophil elastase contributes to the aggravation of pneumococcal pneumonia.
Title: Degradation of EGFR on lung epithelial cells by neutrophil elastase contributes to the aggravation of pneumococcal pneumonia.
ELANE Promotes M2 Macrophage Polarization by Down-Regulating PTEN and Participates in the Lung Cancer Progression.
Title: ELANE Promotes M2 Macrophage Polarization by Down-Regulating PTEN and Participates in the Lung Cancer Progression.
Neutrophil Extracellular Traps Mediate Acute Liver Failure in Regulation of miR-223/Neutrophil Elastase Signaling in Mice.
Title: Neutrophil Extracellular Traps Mediate Acute Liver Failure in Regulation of miR-223/Neutrophil Elastase Signaling in Mice.
Neutrophil elastase selectively kills cancer cells and attenuates tumorigenesis.
Title: Neutrophil elastase selectively kills cancer cells and attenuates tumorigenesis.
Viral mimetic triggers cerebral arteriopathy in juvenile brain via neutrophil elastase and NETosis.
Title: Viral mimetic triggers cerebral arteriopathy in juvenile brain via neutrophil elastase and NETosis.
Neutrophil elastase promotes neointimal hyperplasia by targeting toll-like receptor 4 (TLR4)-NF-kappaB signalling.
Title: Neutrophil elastase promotes neointimal hyperplasia by targeting toll-like receptor 4 (TLR4)-NF-κB signalling.
Neutrophil elastase-regulated macrophage sheddome/secretome and phagocytic failure.
Title: Neutrophil elastase-regulated macrophage sheddome/secretome and phagocytic failure.
Mice Deficient in the IL-1beta Activation Genes Prtn3, Elane, and Casp1 Are Protected Against the Development of Obesity-Induced NAFLD.
Title: Mice Deficient in the IL-1β Activation Genes Prtn3, Elane, and Casp1 Are Protected Against the Development of Obesity-Induced NAFLD.

Submit: New GeneRIF Correction See all GeneRIFs (65)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Chemically induced (ENU) (1)
Endonuclease-mediated (5)
Targeted (3) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables cytokine binding	IEA Inferred from Electronic Annotation more info
enables cytokine binding	ISO Inferred from Sequence Orthology more info
enables endopeptidase activity	ISO Inferred from Sequence Orthology more info
enables heparin binding	ISO Inferred from Sequence Orthology more info
enables peptidase activity	ISO Inferred from Sequence Orthology more info
enables protease binding	IEA Inferred from Electronic Annotation more info
enables protease binding	ISO Inferred from Sequence Orthology more info
enables serine-type endopeptidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables serine-type endopeptidase activity	IDA Inferred from Direct Assay more info	PubMed
enables serine-type endopeptidase activity	ISO Inferred from Sequence Orthology more info
enables transcription corepressor activity	IEA Inferred from Electronic Annotation more info
enables transcription corepressor activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in acute inflammatory response to antigenic stimulus	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within acute inflammatory response to antigenic stimulus	IDA Inferred from Direct Assay more info	PubMed
involved_in biosynthetic process of antibacterial peptides active against Gram-negative bacteria	IEA Inferred from Electronic Annotation more info
involved_in biosynthetic process of antibacterial peptides active against Gram-negative bacteria	ISO Inferred from Sequence Orthology more info
involved_in defense response to bacterium	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within defense response to fungus	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within defense response to fungus	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within leukocyte migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in leukocyte migration involved in inflammatory response	IEA Inferred from Electronic Annotation more info
involved_in leukocyte migration involved in inflammatory response	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of chemokine production	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of chemokine production	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of interleukin-8 production	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of interleukin-8 production	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within neutrophil-mediated killing of fungus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neutrophil-mediated killing of gram-negative bacterium	IEA Inferred from Electronic Annotation more info
involved_in neutrophil-mediated killing of gram-negative bacterium	ISO Inferred from Sequence Orthology more info
involved_in phagocytosis	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within phagocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of immune response	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within positive regulation of immune response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of interleukin-8 production	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of interleukin-8 production	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of leukocyte tethering or rolling	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of leukocyte tethering or rolling	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of smooth muscle cell proliferation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of smooth muscle cell proliferation	ISO Inferred from Sequence Orthology more info
involved_in proteolysis	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within proteolysis	ISO Inferred from Sequence Orthology more info
involved_in proteolysis	ISO Inferred from Sequence Orthology more info
involved_in response to UV	IEA Inferred from Electronic Annotation more info
involved_in response to UV	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within response to lipopolysaccharide	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within response to lipopolysaccharide	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to yeast	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cell surface	IEA Inferred from Electronic Annotation more info
located_in cell surface	ISO Inferred from Sequence Orthology more info
located_in collagen-containing extracellular matrix	HDA more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in extracellular space	IBA Inferred from Biological aspect of Ancestor more info
located_in extracellular space	IEA Inferred from Electronic Annotation more info
located_in extracellular space	ISO Inferred from Sequence Orthology more info
located_in secretory granule	ISO Inferred from Sequence Orthology more info
part_of transcription repressor complex	IEA Inferred from Electronic Annotation more info
part_of transcription repressor complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: neutrophil elastase

Names: elastase 2, neutrophil; leukocyte elastase

NP_056594.2

EC 3.4.21.37

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_015779.2 → NP_056594.2 neutrophil elastase precursor

See identical proteins and their annotated locations for NP_056594.2

Status: REVIEWED

Source sequence(s)

AK143710, DY245521

Consensus CDS

CCDS23994.1

UniProtKB/Swiss-Prot

A6H698, Q3UP87, Q61515

Related

ENSMUSP00000038925.5, ENSMUST00000046091.7

Conserved Domains (2) summary

smart00020
Location:28 → 242

Tryp_SPc; Trypsin-like serine protease

cd00190
Location:29 → 245

Tryp_SPc; Trypsin-like serine protease; Many of these are synthesized as inactive precursor zymogens that are cleaved during limited proteolysis to generate their active forms. Alignment contains also inactive enzymes that have substitutions of the catalytic triad ...