Nsun4 NOL1/NOP2/Sun domain family, member 4 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Nsun4provided by MGI
Official Full Name: NOL1/NOP2/Sun domain family, member 4provided by MGI
Primary source: MGI:MGI:1919431
See related: Ensembl:ENSMUSG00000028706 AllianceGenome:MGI:1919431
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Shtap; 2310010O12Rik; 2810405F18Rik
Summary: Enables small ribosomal subunit rRNA binding activity. Involved in cytosolic ribosome assembly; positive regulation of mitochondrial translation; and rRNA methylation. Located in mitochondrion. Is expressed in several structures, including branchial arch; central nervous system; limb bud; neural ectoderm; and sensory organ. Orthologous to human NSUN4 (NOP2/Sun RNA methyltransferase 4). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in testis adult (RPKM 57.2) and heart adult (RPKM 3.5) See more
Orthologs: human all
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Genomic context

Location:: 4 D1; 4 53.1 cM

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	4	NC_000070.7 (115890202..115911076, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	4	NC_000070.6 (116033002..116053879, complement)

Chromosome 4 - NC_000070.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The presented data suggest that NSUN4 has a key role in controlling a final step in ribosome biogenesis to ensure that only the mature SSU and LSU are assembled.
Title: NSUN4 is a dual function mitochondrial protein required for both methylation of 12S rRNA and coordination of mitoribosomal assembly.
expression and localization of SHTAP in testis and sperm raise the possibility that the SHTAP-CRISP2 complex has a potential role in sperm formation and/or function.
Title: A novel protein, sperm head and tail associated protein (SHTAP), interacts with cysteine-rich secretory protein 2 (CRISP2) during spermatogenesis in the mouse.

Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (2) 1 citation

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables mRNA (cytidine-5-)-methyltransferase activity	ISS Inferred from Sequence or Structural Similarity more info
enables rRNA (cytosine-C5-)-methyltransferase activity	ISO Inferred from Sequence Orthology more info
enables rRNA methyltransferase activity	ISO Inferred from Sequence Orthology more info
enables small ribosomal subunit rRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables tRNA (cytidine-5-)-methyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables tRNA (cytidine-5-)-methyltransferase activity	IEA Inferred from Electronic Annotation more info
enables tRNA binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in cytosolic ribosome assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitochondrial RNA catabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of mitochondrial translation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in rRNA methylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in rRNA methylation	ISO Inferred from Sequence Orthology more info
involved_in tRNA methylation	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
part_of mitochondrial large ribosomal subunit	ISO Inferred from Sequence Orthology more info	PubMed
located_in mitochondrial matrix	ISS Inferred from Sequence or Structural Similarity more info
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: putative methyltransferase NSUN4

Names: 5-methylcytosine tRNA methyltransferase NSUN4; sperm head and tail associated protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001369075.1 → NP_001356004.1 putative methyltransferase NSUN4 isoform b

Status: VALIDATED

Description

Transcript Variant: This variant (2), as well as variant 3, encodes isoform b.

Source sequence(s)

AL627105

Conserved Domains (1) summary

COG0144
Location:103 → 274

RsmB; 16S rRNA C967 or C1407 C5-methylase, RsmB/RsmF family [Translation, ribosomal structure and biogenesis]
NM_001369076.1 → NP_001356005.1 putative methyltransferase NSUN4 isoform b

Status: VALIDATED

Description

Transcript Variant: This variant (3), as well as variant 2, encodes isoform b.

Source sequence(s)

AL627105

Conserved Domains (1) summary

COG0144
Location:103 → 274

RsmB; 16S rRNA C967 or C1407 C5-methylase, RsmB/RsmF family [Translation, ribosomal structure and biogenesis]
NM_028142.5 → NP_082418.1 putative methyltransferase NSUN4 isoform a

See identical proteins and their annotated locations for NP_082418.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longer isoform (a).

Source sequence(s)

AL627105

Consensus CDS

CCDS18502.1

UniProtKB/Swiss-Prot

Q9CZ57, Q9D7F0

Related

ENSMUSP00000030475.2, ENSMUST00000030475.3

Conserved Domains (1) summary

cl17173
Location:163 → 322

AdoMet_MTases; S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes that use S-adenosyl-L-methionine (SAM or AdoMet) as a substrate for methyltransfer, creating the product S-adenosyl-L-homocysteine (AdoHcy). ...