Cenpe centromere protein E [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Cenpeprovided by MGI
Official Full Name: centromere protein Eprovided by MGI
Primary source: MGI:MGI:1098230
See related: Ensembl:ENSMUSG00000045328 AllianceGenome:MGI:1098230
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Kif10; 312kDa; CENP-E; C530022J18
Summary: Enables kinetochore binding activity and protein kinase binding activity. Involved in several processes, including attachment of spindle microtubules to kinetochore; positive regulation of protein kinase activity; and regulation of chromosome segregation. Acts upstream of or within regulation of protein stability and spindle assembly involved in female meiosis I. Located in kinetochore. Part of outer kinetochore. Is expressed in dentate gyrus subgranular zone; testis; and thymus. Human ortholog(s) of this gene implicated in primary autosomal recessive microcephaly 13. Orthologous to human CENPE (centromere protein E). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in CNS E11.5 (RPKM 11.9), liver E14 (RPKM 8.2) and 9 other tissues See more
Orthologs: human all
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Genomic context

Location:: 3 G3; 3 62.55 cM

Exon count:: 46

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	3	NC_000069.7 (134918324..134979301)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	3	NC_000069.6 (135212563..135273540)

Chromosome 3 - NC_000069.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Functional Assessment of Kinesin-7 CENP-E in Spermatocytes using In Vivo Inhibition, Immunofluorescence and Flow Cytometry.
Title: Functional Assessment of Kinesin-7 CENP-E in Spermatocytes using In Vivo Inhibition, Immunofluorescence and Flow Cytometry.
Kinesin-7 CENP-E is essential for chromosome alignment and spindle assembly of mouse spermatocytes.
Title: Kinesin-7 CENP-E is essential for chromosome alignment and spindle assembly of mouse spermatocytes.
CENPE contributes to pulmonary vascular remodeling in pulmonary hypertension.
Title: CENPE contributes to pulmonary vascular remodeling in pulmonary hypertension.
Exacerbating chromosome missegregation in CENP-E+/- mice by reducing levels of another mitotic checkpoint component, Mad2, results in elevated cell death and decreased tumor formation compared with reduction of either protein alone.
Title: Chromosome missegregation rate predicts whether aneuploidy will promote or suppress tumors.
CENP-E stabilises BubR1, thereby impacting meiosis I progression, and mediates bi-orientation by promoting kinetochore reorientation and preventing chromosomal drift towards the poles.
Title: Spindle assembly checkpoint signalling is uncoupled from chromosomal position in mouse oocytes.
Shugoshin 2 is necessary for the loading of MCAK at the inner centromere, but is dispensable for the loading of the outer kinetochore proteins BubR1 and CENP-E.
Title: Sequential assembly of centromeric proteins in male mouse meiosis.
Deletion of the mouse gene CENP-E causes unstable kinetochore-microtubule capture and chromosomal instability. (CENP-E)
Title: Unstable kinetochore-microtubule capture and chromosomal instability following deletion of CENP-E.
results suggest that CENP-E is implicated in the spindle checkpoint, and in chromosome alignment, during the two meiotic divisions in vertebrate males
Title: Expression and behaviour of CENP-E at kinetochores during mouse spermatogenesis.

Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3)
Targeted (5) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables kinetochore binding	IDA Inferred from Direct Assay more info	PubMed
enables kinetochore binding	ISO Inferred from Sequence Orthology more info
enables microtubule binding	IBA Inferred from Biological aspect of Ancestor more info
enables microtubule binding	IEA Inferred from Electronic Annotation more info
enables microtubule binding	ISO Inferred from Sequence Orthology more info
enables microtubule motor activity	IEA Inferred from Electronic Annotation more info
enables microtubule motor activity	ISO Inferred from Sequence Orthology more info
enables protein kinase binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in attachment of mitotic spindle microtubules to kinetochore	ISO Inferred from Sequence Orthology more info
involved_in attachment of mitotic spindle microtubules to kinetochore	ISS Inferred from Sequence or Structural Similarity more info
involved_in attachment of spindle microtubules to kinetochore	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within attachment of spindle microtubules to kinetochore	TAS Traceable Author Statement more info	PubMed
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in chromosome segregation	ISO Inferred from Sequence Orthology more info
involved_in establishment of protein localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in kinetochore assembly	ISO Inferred from Sequence Orthology more info
involved_in lateral attachment of mitotic spindle microtubules to kinetochore	ISO Inferred from Sequence Orthology more info
involved_in lateral attachment of mitotic spindle microtubules to kinetochore	ISS Inferred from Sequence or Structural Similarity more info
involved_in metaphase chromosome alignment	ISO Inferred from Sequence Orthology more info
involved_in metaphase chromosome alignment	ISS Inferred from Sequence or Structural Similarity more info
involved_in microtubule plus-end directed mitotic chromosome migration	ISO Inferred from Sequence Orthology more info
involved_in microtubule-based movement	IBA Inferred from Biological aspect of Ancestor more info
involved_in microtubule-based movement	IEA Inferred from Electronic Annotation more info
involved_in mitotic cell cycle	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic cell cycle	ISO Inferred from Sequence Orthology more info
involved_in mitotic chromosome movement towards spindle pole	ISO Inferred from Sequence Orthology more info
involved_in mitotic metaphase chromosome alignment	ISO Inferred from Sequence Orthology more info
involved_in mitotic metaphase chromosome alignment	ISS Inferred from Sequence or Structural Similarity more info
involved_in mitotic spindle assembly checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic spindle organization	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of attachment of spindle microtubules to kinetochore	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of chromosome segregation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of mitotic metaphase/anaphase transition	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of protein kinase activity	ISO Inferred from Sequence Orthology more info
involved_in regulation of developmental process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of mitotic metaphase/anaphase transition	ISO Inferred from Sequence Orthology more info
involved_in regulation of mitotic nuclear division	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of protein stability	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within spindle assembly involved in female meiosis I	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in chromosome	ISO Inferred from Sequence Orthology more info
located_in chromosome, centromeric region	ISO Inferred from Sequence Orthology more info	PubMed
located_in condensed chromosome, centromeric region	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in intercellular bridge	ISO Inferred from Sequence Orthology more info
located_in intracellular membrane-bounded organelle	ISO Inferred from Sequence Orthology more info
located_in kinetochore	IDA Inferred from Direct Assay more info	PubMed
located_in kinetochore	ISO Inferred from Sequence Orthology more info
located_in kinetochore microtubule	ISO Inferred from Sequence Orthology more info
is_active_in microtubule	IBA Inferred from Biological aspect of Ancestor more info
located_in microtubule	ISO Inferred from Sequence Orthology more info
located_in microtubule cytoskeleton	ISO Inferred from Sequence Orthology more info
located_in midbody	ISO Inferred from Sequence Orthology more info
located_in mitotic spindle	ISO Inferred from Sequence Orthology more info
located_in mitotic spindle midzone	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
part_of outer kinetochore	IDA Inferred from Direct Assay more info	PubMed
located_in spindle	IEA Inferred from Electronic Annotation more info
located_in spindle midzone	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: centromere-associated protein E

Names: N-7 kinesin; centromere autoantigen E; centromeric protein E; kinesin 10; kinesin superfamily protein 10; motor domain of KIF10

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_173762.4 → NP_776123.3 centromere-associated protein E

Status: VALIDATED

Source sequence(s)

AC104874, AK133445, AY493378, CN534554, CN704540

Consensus CDS

CCDS51072.1

UniProtKB/Swiss-Prot

O35059, Q3KQQ6, Q3V044, Q6PD00, Q6RT24, Q7TPX4, Q80YB4, Q8BWX6

UniProtKB/TrEMBL

E9QKK1

Related

ENSMUSP00000057938.8, ENSMUST00000062893.12

Conserved Domains (6) summary

pfam05483
Location:1100 → 1841

SCP-1; Synaptonemal complex protein 1 (SCP-1)

smart00129
Location:6 → 336

KISc; Kinesin motor, catalytic domain. ATPase

COG0419
Location:1570 → 2128

SbcC; DNA repair exonuclease SbcCD ATPase subunit [Replication, recombination and repair]

TIGR02168
Location:586 → 1436

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

cd01374
Location:6 → 329

KISc_CENP_E; Kinesin motor domain, CENP-E/KIP2-like subgroup

pfam06156
Location:1041 → 1119

DUF972; Protein of unknown function (DUF972)