Ppp3ca protein phosphatase 3, catalytic subunit, alpha isoform [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ppp3caprovided by MGI
Official Full Name: protein phosphatase 3, catalytic subunit, alpha isoformprovided by MGI
Primary source: MGI:MGI:107164
See related: Ensembl:ENSMUSG00000028161 AllianceGenome:MGI:107164
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: CN; CnA; Caln; Calna; 2900074D19Rik
Summary: Enables calmodulin binding activity and calmodulin-dependent protein phosphatase activity. Involved in several processes, including positive regulation of cell differentiation; positive regulation of transport; and regulation of gene expression. Acts upstream of or within several processes, including G1/S transition of mitotic cell cycle; calcineurin-NFAT signaling cascade; and regulation of DNA-templated transcription. Located in several cellular components, including Z disc; cytosol; and mitochondrion. Part of calcineurin complex. Is active in Schaffer collateral - CA1 synapse and glutamatergic synapse. Is expressed in several structures, including central nervous system; genitourinary system; gut gland; jaw; and thymus primordium. Used to study dilated cardiomyopathy. Human ortholog(s) of this gene implicated in developmental and epileptic encephalopathy 91. Orthologous to human PPP3CA (protein phosphatase 3 catalytic subunit alpha). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Broad expression in cortex adult (RPKM 101.0), frontal lobe adult (RPKM 75.5) and 17 other tissues See more
Orthologs: human all
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Genomic context

Location:: 3 G3; 3 63.34 cM

Exon count:: 17

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	3	NC_000069.7 (136375778..136643488)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	3	NC_000069.6 (136669714..136937727)

Chromosome 3 - NC_000069.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

WNT16b promotes the proliferation and self-renewal of human limbal epithelial stem/progenitor cells via activating the calcium/calcineurin A/NFATC2 pathway.
Title: WNT16b promotes the proliferation and self-renewal of human limbal epithelial stem/progenitor cells via activating the calcium/calcineurin A/NFATC2 pathway.
Calcineurin dephosphorylates topoisomerase IIbeta and regulates the formation of neuronal-activity-induced DNA breaks.
Title: Calcineurin dephosphorylates topoisomerase IIβ and regulates the formation of neuronal-activity-induced DNA breaks.
Coexpression of calcineurin A and B subunits in various subcellular and synaptic compartments of cerebellar neurons and glia with particular abundance at parallel fiber-Purkinje cell synapses.
Title: Coexpression of calcineurin A and B subunits in various subcellular and synaptic compartments of cerebellar neurons and glia with particular abundance at parallel fiber-Purkinje cell synapses.
Deletion of calcineurin from GFAP-expressing astrocytes impairs excitability of cerebellar and hippocampal neurons through astroglial Na(+) /K(+) ATPase.
Title: Deletion of calcineurin from GFAP-expressing astrocytes impairs excitability of cerebellar and hippocampal neurons through astroglial Na(+) /K(+) ATPase.
Bmp2 is a downstream target of the calcineurin/NFAT pathway in lung endothelium, potently inhibiting cancer cell growth by stimulating differentiation.
Title: Endothelial Calcineurin Signaling Restrains Metastatic Outgrowth by Regulating Bmp2.
IFNG utilizes the PPP3-TFEB signaling axis for inducing autophagy and regulating mycobacterial growth.
Title: Antimycobacterial effect of IFNG (interferon gamma)-induced autophagy depends on HMOX1 (heme oxygenase 1)-mediated increase in intracellular calcium levels and modulation of PPP3/calcineurin-TFEB (transcription factor EB) axis.
within interneurons, Calcineurin, a calcium-dependent protein phosphatase, plays a critical role in sequentially linking activity to maturation (E15-P5) and survival (P5-P20).
Title: Activity Regulates Cell Death within Cortical Interneurons through a Calcineurin-Dependent Mechanism.
calcineurin in astrocytes may be activated in response to neuronal activity.
Title: Neuronal Activity-Dependent Activation of Astroglial Calcineurin in Mouse Primary Hippocampal Cultures.
calcineurin is a key regulator of Wnt5a-induced AQP2 activation without affecting intracellular cAMP level and PKA activity.
Title: Wnt5a induces renal AQP2 expression by activating calcineurin signalling pathway.
Redirected splicing of calcineurin A to the fetal isoforms in adult muscle and in differentiated C2C12 slows the timing of muscle relaxation, promotes nuclear localization of calcineurin target Nfatc3, and/or affects expression of Nfatc transcription targets.
Title: Extensive alternative splicing transitions during postnatal skeletal muscle development are required for calcium handling functions.

Submit: New GeneRIF Correction See all GeneRIFs (71)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3)
Gene trapped (1)
Targeted (4) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Ppp3ca (e-PCR), detects polymorphism
- UniSTS:546560

D3Mit146 (e-PCR), detects polymorphism
- UniSTS:129557

PPP3CA (e-PCR)
- UniSTS:503449

Ppp3ca (e-PCR), detects polymorphism
- UniSTS:499105

WI-16419 (e-PCR)
- UniSTS:61859

AI841391 (e-PCR)
- UniSTS:165299

J05479 (e-PCR)
- UniSTS:124858

SHGC-67255 (e-PCR)
- UniSTS:14011

AW413465 (e-PCR)
- UniSTS:178938

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATPase binding	IEA Inferred from Electronic Annotation more info
enables ATPase binding	ISO Inferred from Sequence Orthology more info
enables MAP kinase serine/threonine phosphatase activity	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II CTD heptapeptide repeat S2 phosphatase activity	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II CTD heptapeptide repeat S5 phosphatase activity	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II CTD heptapeptide repeat S7 phosphatase activity	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II CTD heptapeptide repeat T4 phosphatase activity	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II CTD heptapeptide repeat Y1 phosphatase activity	IEA Inferred from Electronic Annotation more info
enables calcium-dependent protein serine/threonine phosphatase activity	ISO Inferred from Sequence Orthology more info
enables calcium-dependent protein serine/threonine phosphatase activity	TAS Traceable Author Statement more info
enables calmodulin binding	IBA Inferred from Biological aspect of Ancestor more info
enables calmodulin binding	IDA Inferred from Direct Assay more info	PubMed
enables calmodulin binding	ISO Inferred from Sequence Orthology more info
enables calmodulin-dependent protein phosphatase activity	IBA Inferred from Biological aspect of Ancestor more info
enables calmodulin-dependent protein phosphatase activity	IDA Inferred from Direct Assay more info	PubMed
enables calmodulin-dependent protein phosphatase activity	ISO Inferred from Sequence Orthology more info
enables calmodulin-dependent protein phosphatase activity	ISS Inferred from Sequence or Structural Similarity more info
enables cyclosporin A binding	ISO Inferred from Sequence Orthology more info
enables enzyme binding	ISO Inferred from Sequence Orthology more info
enables histone H2AXS140 phosphatase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables myosin phosphatase activity	IEA Inferred from Electronic Annotation more info
enables phosphoprotein phosphatase activity	IDA Inferred from Direct Assay more info	PubMed
enables phosphoprotein phosphatase activity	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein dimerization activity	IEA Inferred from Electronic Annotation more info
enables protein dimerization activity	ISO Inferred from Sequence Orthology more info
enables protein serine/threonine phosphatase activity	ISO Inferred from Sequence Orthology more info
enables protein-containing complex binding	IEA Inferred from Electronic Annotation more info
enables protein-containing complex binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within G1/S transition of mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in calcineurin-NFAT signaling cascade	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within calcineurin-NFAT signaling cascade	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within calcineurin-NFAT signaling cascade	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within calcineurin-NFAT signaling cascade	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in calcineurin-NFAT signaling cascade	ISO Inferred from Sequence Orthology more info
involved_in calcineurin-mediated signaling	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within calcium ion transport	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within calcium-mediated signaling	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within cardiac muscle hypertrophy in response to stress	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular response to glucose stimulus	IEA Inferred from Electronic Annotation more info
involved_in cellular response to glucose stimulus	ISO Inferred from Sequence Orthology more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
acts_upstream_of dendrite morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within dephosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in epidermis development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within excitatory postsynaptic potential	IGI Inferred from Genetic Interaction more info	PubMed
involved_in keratinocyte differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within modulation of chemical synaptic transmission	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in modulation of chemical synaptic transmission	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within multicellular organismal response to stress	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of angiotensin-activated signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of angiotensin-activated signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of calcium ion import across plasma membrane	NAS Non-traceable Author Statement more info	PubMed
acts_upstream_of_or_within negative regulation of chromatin binding	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of dendrite morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of gene expression	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of insulin secretion	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of insulin secretion	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in peptidyl-serine dephosphorylation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of DNA-binding transcription factor activity	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of activated T cell proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of calcineurin-NFAT signaling cascade	NAS Non-traceable Author Statement more info	PubMed
involved_in positive regulation of calcium ion import across plasma membrane	NAS Non-traceable Author Statement more info	PubMed
involved_in positive regulation of cardiac muscle hypertrophy	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of cardiac muscle hypertrophy in response to stress	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of cell adhesion	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell migration	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of cell migration	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of connective tissue replacement	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of gene expression	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of glomerulus development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of osteoblast differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of osteoclast differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of saliva secretion	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in postsynaptic modulation of chemical synaptic transmission	IDA Inferred from Direct Assay more info	PubMed
involved_in postsynaptic modulation of chemical synaptic transmission	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein dephosphorylation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within protein dephosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein dephosphorylation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within protein import into nucleus	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within protein import into nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein import into nucleus	ISO Inferred from Sequence Orthology more info
involved_in regulation of cell proliferation involved in kidney morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in renal filtration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to amphetamine	IEA Inferred from Electronic Annotation more info
involved_in response to calcium ion	ISO Inferred from Sequence Orthology more info
involved_in response to calcium ion	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within skeletal muscle fiber development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in skeletal muscle fiber development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within transition between fast and slow fiber	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
is_active_in Schaffer collateral - CA1 synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in Schaffer collateral - CA1 synapse	IMP Inferred from Mutant Phenotype more info	PubMed
located_in Z disc	IDA Inferred from Direct Assay more info	PubMed
part_of calcineurin complex	IBA Inferred from Biological aspect of Ancestor more info
part_of calcineurin complex	IDA Inferred from Direct Assay more info	PubMed
part_of calcineurin complex	ISO Inferred from Sequence Orthology more info
part_of calcineurin complex	NAS Non-traceable Author Statement more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasmic side of plasma membrane	IEA Inferred from Electronic Annotation more info
colocalizes_with cytoplasmic side of plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	NAS Non-traceable Author Statement more info	PubMed
located_in cytosol	TAS Traceable Author Statement more info
located_in dendritic spine	IEA Inferred from Electronic Annotation more info
located_in dendritic spine	ISO Inferred from Sequence Orthology more info
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
part_of protein serine/threonine phosphatase complex	NAS Non-traceable Author Statement more info	PubMed
located_in sarcolemma	IEA Inferred from Electronic Annotation more info
located_in slit diaphragm	IEA Inferred from Electronic Annotation more info
located_in slit diaphragm	ISO Inferred from Sequence Orthology more info
is_active_in synapse	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: protein phosphatase 3 catalytic subunit alpha

Names: CAM-PRP catalytic subunit; CNA alpha; PP2B alpha 1; PP2BA alpha; calcineurin A alpha

NP_001280551.1

EC 3.1.3.16

NP_032939.1

EC 3.1.3.16

XP_017174981.1

EC 3.1.3.16

XP_017174982.1

EC 3.1.3.16

XP_030108322.1

EC 3.1.3.16

XP_030108323.1

EC 3.1.3.16

XP_030108324.1

EC 3.1.3.16

XP_036018837.1

EC 3.1.3.16

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001293622.2 → NP_001280551.1 protein phosphatase 3 catalytic subunit alpha isoform 2

Status: VALIDATED

Source sequence(s)

AC116384, AC124177, AC129775

Consensus CDS

CCDS80027.1

UniProtKB/Swiss-Prot

P63328

Related

ENSMUSP00000071040.8, ENSMUST00000070198.14

Conserved Domains (1) summary

cd07416
Location:41 → 345

MPP_PP2B; PP2B, metallophosphatase domain
NM_001428644.1 → NP_001415573.1 protein phosphatase 3 catalytic subunit alpha isoform 3

Status: VALIDATED

Source sequence(s)

AC116384, AC124177, AC127681, AC129775
NM_001428645.1 → NP_001415574.1 protein phosphatase 3 catalytic subunit alpha isoform 4

Status: VALIDATED

Source sequence(s)

AC116384, AC124177, AC127681, AC129775
NM_001428646.1 → NP_001415575.1 protein phosphatase 3 catalytic subunit alpha isoform 4

Status: VALIDATED

Source sequence(s)

AC116384, AC124177, AC127681, AC129775
NM_008913.6 → NP_032939.1 protein phosphatase 3 catalytic subunit alpha isoform 1

Status: VALIDATED

Source sequence(s)

AC116384, AC124177, AC129775

Consensus CDS

CCDS17860.1

UniProtKB/Swiss-Prot

B2RRX2, P12816, P20652, P63328, Q3UCU1, Q64135

Related

ENSMUSP00000053101.9, ENSMUST00000056758.9

Conserved Domains (1) summary

cd07416
Location:41 → 345

MPP_PP2B; PP2B, metallophosphatase domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000069.7 Reference GRCm39 C57BL/6J

Range

136375778..136643488

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_030252463.2 → XP_030108323.1 protein phosphatase 3 catalytic subunit alpha isoform X1

Conserved Domains (1) summary

cd07416
Location:1 → 295

MPP_PP2B; PP2B, metallophosphatase domain
XM_030252464.2 → XP_030108324.1 protein phosphatase 3 catalytic subunit alpha isoform X2

Conserved Domains (1) summary

cd07416
Location:1 → 295

MPP_PP2B; PP2B, metallophosphatase domain
XM_030252462.2 → XP_030108322.1 protein phosphatase 3 catalytic subunit alpha isoform X1

Conserved Domains (1) summary

cd07416
Location:1 → 295

MPP_PP2B; PP2B, metallophosphatase domain
XM_017319493.3 → XP_017174982.1 protein phosphatase 3 catalytic subunit alpha isoform X2

Conserved Domains (1) summary

cd07416
Location:1 → 295

MPP_PP2B; PP2B, metallophosphatase domain
XM_036162944.1 → XP_036018837.1 protein phosphatase 3 catalytic subunit alpha isoform X1

Conserved Domains (1) summary

cd07416
Location:1 → 295

MPP_PP2B; PP2B, metallophosphatase domain
XM_017319492.3 → XP_017174981.1 protein phosphatase 3 catalytic subunit alpha isoform X1

Conserved Domains (1) summary

cd07416
Location:1 → 295

MPP_PP2B; PP2B, metallophosphatase domain