Washc1 WASH complex subunit 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Washc1provided by MGI
Official Full Name: WASH complex subunit 1provided by MGI
Primary source: MGI:MGI:1916017
See related: Ensembl:ENSMUSG00000024101 AllianceGenome:MGI:1916017
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Wash; ORF19; Wash1; 1110049F14Rik
Summary: Enables gamma-tubulin binding activity and phosphatidylinositol 3-kinase inhibitor activity. Involved in several processes, including Arp2/3 complex-mediated actin nucleation; meiotic nuclear division; and oocyte maturation. Acts upstream of or within several processes, including low-density lipoprotein particle clearance; positive regulation of cholesterol import; and positive regulation of protein localization to cell surface. Located in autophagosome; centrosome; and endosome. Part of BLOC-1 complex and WASH complex. Is expressed in several structures, including brain; genitourinary system; immune system; liver; and lung. Orthologous to several human genes including WASHC1 (WASH complex subunit 1). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in thymus adult (RPKM 14.2), ovary adult (RPKM 14.1) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 17 E1.1; 17 35.26 cM

Exon count:: 12

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	17	NC_000083.7 (66418524..66427498)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	17	NC_000083.6 (66111529..66120503)

Chromosome 17 - NC_000083.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Changes in bile acid composition are correlated with reduced intestinal cholesterol uptake in intestine-specific WASH-deficient mice.
Title: Changes in bile acid composition are correlated with reduced intestinal cholesterol uptake in intestine-specific WASH-deficient mice.
Inactivation of Rho GTPases by Burkholderia cenocepacia Induces a WASH-Mediated Actin Polymerization that Delays Phagosome Maturation.
Title: Inactivation of Rho GTPases by Burkholderia cenocepacia Induces a WASH-Mediated Actin Polymerization that Delays Phagosome Maturation.
The hepatic WASH complex is required for efficient plasma LDL and HDL cholesterol clearance.
Title: The hepatic WASH complex is required for efficient plasma LDL and HDL cholesterol clearance.
WASH deficiency impairs the association of RNF2 with AMBRA1 to impede AMBRA1 degradation.
Title: RNF2 is recruited by WASH to ubiquitinate AMBRA1 leading to downregulation of autophagy.
WASH complex regulates Arp2/3 complex and is required for cytokinesis and polar body extrusion.
Title: WASH complex regulates Arp2/3 complex for actin-based polar body extrusion in mouse oocytes.
WASH is required for the differentiation commitment of hematopoietic stem cells by acting as an upstream regulator to modulate c-Myc transcription.
Title: WASH is required for the differentiation commitment of hematopoietic stem cells in a c-Myc-dependent manner.
WASH can suppress Beclin 1 ubiquitination to inactivate Vps34 activity leading to suppression of autophagy.
Title: WASH inhibits autophagy through suppression of Beclin 1 ubiquitination.
WASH is a newly recognized regulator of T cell receptor, CD28, LFA-1, and GLUT1 endosome-to-membrane recycling.
Title: WASH knockout T cells demonstrate defective receptor trafficking, proliferation, and effector function.
WASH-mediated F-actin is requisite for the integrity of both endosomal and lysosomal networks in mammalian cells.
Title: Trafficking defects in WASH-knockout fibroblasts originate from collapsed endosomal and lysosomal networks.
WASH is a bimodular protein and a component of the BLOC-1 complex in which the C terminus is involved in Arp2/3-mediated actin nucleation, whereas the N-terminal portion is required for its regulation and localization in the cells
Title: Functional characterization of Wiskott-Aldrich syndrome protein and scar homolog (WASH), a bi-modular nucleation-promoting factor able to interact with biogenesis of lysosome-related organelle subunit 2 (BLOS2) and gamma-tubulin.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (4) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables actin binding	IEA Inferred from Electronic Annotation more info
enables alpha-tubulin binding	IBA Inferred from Biological aspect of Ancestor more info
enables alpha-tubulin binding	ISO Inferred from Sequence Orthology more info
enables alpha-tubulin binding	ISS Inferred from Sequence or Structural Similarity more info
enables gamma-tubulin binding	IBA Inferred from Biological aspect of Ancestor more info
enables gamma-tubulin binding	IDA Inferred from Direct Assay more info	PubMed
enables low-density lipoprotein particle receptor binding	ISO Inferred from Sequence Orthology more info	PubMed
enables phosphatidylinositol 3-kinase inhibitor activity	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin protein ligase binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in Arp2/3 complex-mediated actin nucleation	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within Arp2/3 complex-mediated actin nucleation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in Arp2/3 complex-mediated actin nucleation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in T cell proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dendritic cell antigen processing and presentation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in endocytic recycling	IBA Inferred from Biological aspect of Ancestor more info
involved_in endocytic recycling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in endosomal transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in endosomal transport	NAS Non-traceable Author Statement more info	PubMed
acts_upstream_of_or_within endosome organization	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within endosome to plasma membrane protein transport	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within endosome to plasma membrane protein transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in exocytosis	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within low-density lipoprotein particle clearance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic spindle assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of autophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in oocyte maturation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in polar body extrusion after meiotic divisions	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of cholesterol import	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of protein localization to cell surface	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of protein localization to cell surface	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of protein localization to cell surface	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of protein localization to cell surface	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to cell surface	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of Arp2/3 complex-mediated actin nucleation	NAS Non-traceable Author Statement more info	PubMed
acts_upstream_of_or_within regulation of actin filament polymerization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of immune response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of protein ubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in retrograde transport, endosome to Golgi	IBA Inferred from Biological aspect of Ancestor more info
involved_in retrograde transport, endosome to Golgi	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
part_of BLOC-1 complex	IDA Inferred from Direct Assay more info	PubMed
part_of WASH complex	IBA Inferred from Biological aspect of Ancestor more info
part_of WASH complex	IDA Inferred from Direct Assay more info	PubMed
part_of WASH complex	ISO Inferred from Sequence Orthology more info
part_of WASH complex	ISS Inferred from Sequence or Structural Similarity more info
part_of WASH complex	NAS Non-traceable Author Statement more info	PubMed
located_in autophagosome	IDA Inferred from Direct Assay more info	PubMed
located_in centriole	IEA Inferred from Electronic Annotation more info
located_in centrosome	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IEA Inferred from Electronic Annotation more info
is_active_in early endosome	IBA Inferred from Biological aspect of Ancestor more info
located_in early endosome	IDA Inferred from Direct Assay more info	PubMed
located_in early endosome	ISO Inferred from Sequence Orthology more info
located_in early endosome	ISS Inferred from Sequence or Structural Similarity more info
located_in early endosome membrane	NAS Non-traceable Author Statement more info	PubMed
located_in endosome	IDA Inferred from Direct Assay more info	PubMed
part_of exocyst	ISO Inferred from Sequence Orthology more info
located_in filopodium	ISO Inferred from Sequence Orthology more info
located_in lamellipodium	ISO Inferred from Sequence Orthology more info
located_in late endosome	IDA Inferred from Direct Assay more info	PubMed
is_active_in recycling endosome	IBA Inferred from Biological aspect of Ancestor more info
located_in recycling endosome	IDA Inferred from Direct Assay more info	PubMed
located_in recycling endosome	ISO Inferred from Sequence Orthology more info
located_in recycling endosome membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: WASH complex subunit 1

Names: WAS protein family homolog 1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001037757.1 → NP_001032846.1 WASH complex subunit 1

See identical proteins and their annotated locations for NP_001032846.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein.

Source sequence(s)

AC119957, AK159820, BY339670

Consensus CDS

CCDS28944.1

UniProtKB/Swiss-Prot

Q3U473, Q8VDD8

Related

ENSMUSP00000112255.3, ENSMUST00000116556.4

Conserved Domains (1) summary

pfam11945
Location:15 → 296

WASH_WAHD; WAHD domain of WASH complex
NM_026833.1 → NP_081109.1 WASH complex subunit 1

See identical proteins and their annotated locations for NP_081109.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) and variant 2 encode the same protein.

Source sequence(s)

AC119957, AK159820, BY275336

Consensus CDS

CCDS28944.1

UniProtKB/Swiss-Prot

Q3U473, Q8VDD8

Related

ENSMUSP00000072220.7, ENSMUST00000072383.14

Conserved Domains (1) summary

pfam11945
Location:15 → 296

WASH_WAHD; WAHD domain of WASH complex

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000083.7 Reference GRCm39 C57BL/6J

Range

66418524..66427498

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_030250021.1 → XP_030105881.1 WASH complex subunit 1 isoform X1

Conserved Domains (1) summary

pfam11945
Location:15 → 296

WASH_WAHD; WAHD domain of WASH complex
XM_030250022.1 → XP_030105882.1 WASH complex subunit 1 isoform X1

Conserved Domains (1) summary

pfam11945
Location:15 → 296

WASH_WAHD; WAHD domain of WASH complex
XM_006524859.2 → XP_006524922.1 WASH complex subunit 1 isoform X2

See identical proteins and their annotated locations for XP_006524922.1

UniProtKB/Swiss-Prot

Q3U473, Q8VDD8

Conserved Domains (1) summary

pfam11945
Location:15 → 296

WASH_WAHD; WAHD domain of WASH complex
XM_006524860.5 → XP_006524923.1 WASH complex subunit 1 isoform X2

See identical proteins and their annotated locations for XP_006524923.1

UniProtKB/Swiss-Prot

Q3U473, Q8VDD8

Conserved Domains (1) summary

pfam11945
Location:15 → 296

WASH_WAHD; WAHD domain of WASH complex
XM_030250023.2 → XP_030105883.1 WASH complex subunit 1 isoform X1

Conserved Domains (1) summary

pfam11945
Location:15 → 296

WASH_WAHD; WAHD domain of WASH complex
XM_030250024.1 → XP_030105884.1 WASH complex subunit 1 isoform X1

Conserved Domains (1) summary

pfam11945
Location:15 → 296

WASH_WAHD; WAHD domain of WASH complex