Tnks2 tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Tnks2provided by MGI
Official Full Name: tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2provided by MGI
Primary source: MGI:MGI:1921743
See related: Ensembl:ENSMUSG00000024811 AllianceGenome:MGI:1921743
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: ARTD6; Tank2; Parp5b; TNKS-2; 5430432P15Rik
Summary: Predicted to enable NAD+-protein poly-ADP-ribosyltransferase activity and enzyme binding activity. Acts upstream of or within regulation of growth. Predicted to be located in nuclear envelope; pericentriolar material; and perinuclear region of cytoplasm. Predicted to be active in cytoplasm and nucleus. Is expressed in several structures, including alimentary system; brain; early embryo; eye; and genitourinary system. Orthologous to human TNKS2 (tankyrase 2). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Ubiquitous expression in placenta adult (RPKM 24.7), CNS E11.5 (RPKM 20.9) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 19 C2; 19 31.96 cM

Exon count:: 28

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	19	NC_000085.7 (36811583..36870877)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	19	NC_000085.6 (36834129..36893477)

Chromosome 19 - NC_000085.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Developmentally Programmed Tankyrase Activity Upregulates beta-Catenin and Licenses Progression of Embryonic Genome Activation.
Title: Developmentally Programmed Tankyrase Activity Upregulates β-Catenin and Licenses Progression of Embryonic Genome Activation.
Study using Villin-creERT2;Tnks1-/-;Tnks2fl/fl mice, which harbored conventional Tnks1 deletion and inducible intestinal epithelium-specific Tnks2 knockout, shows that tankyrases regulate adult intestinal Lgr5+ stem cells and epithelium homeostasis by preventing cell death and promoting cell proliferation.
Title: Tankyrases maintain homeostasis of intestinal epithelium by preventing cell death.
Tank1/Tank2 inhibition aggravates kidney injury in the absence of CD2AP.
Title: Tankyrase inhibition aggravates kidney injury in the absence of CD2AP.
a new function of RNF146 and tankyrase in stabilizing the Crumbs complex through downregulation of AMOT proteins at the apical membrane, is reported.
Title: The RNF146 and tankyrase pathway maintains the junctional Crumbs complex through regulation of angiomotin.
These structural insights will be invaluable for the functional and biophysical characterization of TNKS1/2, including the role of TNKS oligomerization in protein poly(ADP-ribosyl)ation (PARylation) and PARylation-dependent ubiquitylation.
Title: Structural insights into SAM domain-mediated tankyrase oligomerization.
TNKS2 is required in insulin-stimulated glucose disposal.
Title: The Axin/TNKS complex interacts with KIF3A and is required for insulin-stimulated GLUT4 translocation.
Study shows that TNKS2, regulates 3BP2 stability through ADP-ribosylation and subsequent ubiquitylation in osteoclasts; cherubism mutations uncouple 3BP2 from Tankyrase-mediated protein destruction.
Title: Loss of Tankyrase-mediated destruction of 3BP2 is the underlying pathogenic mechanism of cherubism.
Data show that Tnks1 and 2 are broadly expressed during mouse development and are essential during kidney and lung development. In the kidney, blockage of tankyrase activity phenocopies the effect of blocking production of all Wnt ligands.
Title: Tankyrase is necessary for canonical Wnt signaling during kidney development.
tankyrase 2is essential but redundant for mouse embryonic development
Title: Tankyrase 1 and tankyrase 2 are essential but redundant for mouse embryonic development.
Tnks2 has a role in normal growth and development but is not essential for telomere length maintenance or telomere capping in mice.
Title: Tankyrase 2 poly(ADP-ribose) polymerase domain-deleted mice exhibit growth defects but have normal telomere length and capping.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Targeted (7) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AI662480 (e-PCR)
- UniSTS:166147

NoName (e-PCR)
- UniSTS:237930

RH124424 (e-PCR)
- UniSTS:161246

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables NAD+-histone H2BE18 glutamate ADP-ribosyltransferase activity	IEA Inferred from Electronic Annotation more info
enables NAD+-histone H2BE2 glutamate ADP-ribosyltransferase activity	IEA Inferred from Electronic Annotation more info
enables NAD+-histone H2BE35 glutamate ADP-ribosyltransferase activity	IEA Inferred from Electronic Annotation more info
enables NAD+-protein ADP-ribosyltransferase activity	ISO Inferred from Sequence Orthology more info
enables NAD+-protein ADP-ribosyltransferase activity	ISS Inferred from Sequence or Structural Similarity more info
enables NAD+-protein poly-ADP-ribosyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables NAD+-protein poly-ADP-ribosyltransferase activity	ISO Inferred from Sequence Orthology more info
enables NAD+-protein poly-ADP-ribosyltransferase activity	ISS Inferred from Sequence or Structural Similarity more info
enables NAD+-protein-aspartate ADP-ribosyltransferase activity	IEA Inferred from Electronic Annotation more info
enables enzyme binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleotidyltransferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in Wnt signaling pathway	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within multicellular organism growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of telomere maintenance via telomere lengthening	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of canonical Wnt signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of canonical Wnt signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of canonical Wnt signaling pathway	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of telomere capping	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of telomere capping	ISO Inferred from Sequence Orthology more info
involved_in protein auto-ADP-ribosylation	ISO Inferred from Sequence Orthology more info
involved_in protein localization to chromosome, telomeric region	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein localization to chromosome, telomeric region	ISO Inferred from Sequence Orthology more info
involved_in protein poly-ADP-ribosylation	ISO Inferred from Sequence Orthology more info
involved_in protein poly-ADP-ribosylation	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein polyubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein polyubiquitination	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within regulation of multicellular organism growth	IMP Inferred from Mutant Phenotype more info	PubMed
NOT acts_upstream_of_or_within telomere maintenance	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within telomere maintenance	ISS Inferred from Sequence or Structural Similarity more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi membrane	IEA Inferred from Electronic Annotation more info
located_in chromosome, telomeric region	IEA Inferred from Electronic Annotation more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in nuclear envelope	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in pericentriolar material	ISO Inferred from Sequence Orthology more info
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: poly [ADP-ribose] polymerase tankyrase-2

Names: ADP-ribosyltransferase diphtheria toxin-like 6; TRF1-interacting ankyrin-related ADP-ribose polymerase 2; protein poly-ADP-ribosyltransferase tankyrase-2; tankyrase II; tankyrase-2; tankyrase-like protein; tankyrase-related protein

NP_001157107.1

EC 2.4.2.30

XP_006527471.1

EC 2.4.2.30

XP_006527472.1

EC 2.4.2.30

XP_011245684.1

EC 2.4.2.30

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001163635.1 → NP_001157107.1 poly [ADP-ribose] polymerase tankyrase-2

See identical proteins and their annotated locations for NP_001157107.1

Status: VALIDATED

Source sequence(s)

AK047094, AK149368, AK163335, BC063101, BY710137, CN531910

Consensus CDS

CCDS50426.1

UniProtKB/Swiss-Prot

Q3UES3, Q6P537, Q8BXH7

UniProtKB/TrEMBL

A0AA74KUV1

Related

ENSMUSP00000025729.6, ENSMUST00000025729.12

Conserved Domains (8) summary

cd09524
Location:871 → 936

SAM_tankyrase1,2; SAM domain of tankyrase1,2 subfamily

smart00454
Location:877 → 933

SAM; Sterile alpha motif

cd00204
Location:518 → 643

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

cd01438
Location:938 → 1160

tankyrase_like; Tankyrases interact with the telomere reverse transcriptase complex (TERT). Tankyrase 1 poly-ADP-ribosylates Telomere Repeat Binding Factor 1 (TRF1) while Tankyrase 2 can poly-ADP-ribosylate itself or TRF1. The tankyrases also contain multiple ankyrin ...

pfam00644
Location:952 → 1155

PARP; Poly(ADP-ribose) polymerase catalytic domain

pfam12796
Location:530 → 622

Ank_2; Ankyrin repeats (3 copies)

pfam13637
Location:28 → 78

Ank_4; Ankyrin repeats (many copies)

sd00045
Location:528 → 556

ANK; ANK repeat [structural motif]

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000085.7 Reference GRCm39 C57BL/6J

Range

36811583..36870877

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_011247382.3 → XP_011245684.1 poly [ADP-ribose] polymerase tankyrase-2 isoform X3

Conserved Domains (4) summary

PTZ00322
Location:27 → 113

PTZ00322; 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase; Provisional

PHA02876
Location:73 → 458

PHA02876; ankyrin repeat protein; Provisional

PHA03095
Location:494 → 784

PHA03095; ankyrin-like protein; Provisional

sd00045
Location:528 → 556

ANK; ANK repeat [structural motif]
XM_006527409.5 → XP_006527472.1 poly [ADP-ribose] polymerase tankyrase-2 isoform X2

UniProtKB/TrEMBL

A0AA74KUV1

Conserved Domains (5) summary

PTZ00322
Location:27 → 113

PTZ00322; 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase; Provisional

PHA02876
Location:73 → 458

PHA02876; ankyrin repeat protein; Provisional

cd09524
Location:808 → 873

SAM_tankyrase1,2; SAM domain of tankyrase1,2 subfamily

cd01438
Location:875 → 1097

tankyrase_like; Tankyrases interact with the telomere reverse transcriptase complex (TERT). Tankyrase 1 poly-ADP-ribosylates Telomere Repeat Binding Factor 1 (TRF1) while Tankyrase 2 can poly-ADP-ribosylate itself or TRF1. The tankyrases also contain multiple ankyrin ...

sd00045
Location:528 → 556

ANK; ANK repeat [structural motif]
XM_006527408.5 → XP_006527471.1 poly [ADP-ribose] polymerase tankyrase-2 isoform X1

UniProtKB/TrEMBL

A0AA74KUV1

Conserved Domains (5) summary

cd09524
Location:814 → 879

SAM_tankyrase1,2; SAM domain of tankyrase1,2 subfamily

PHA03095
Location:437 → 745

PHA03095; ankyrin-like protein; Provisional

cd01438
Location:881 → 1103

tankyrase_like; Tankyrases interact with the telomere reverse transcriptase complex (TERT). Tankyrase 1 poly-ADP-ribosylates Telomere Repeat Binding Factor 1 (TRF1) while Tankyrase 2 can poly-ADP-ribosylate itself or TRF1. The tankyrases also contain multiple ankyrin ...

sd00045
Location:471 → 499

ANK; ANK repeat [structural motif]

pfam12796
Location:248 → 404

Ank_2; Ankyrin repeats (3 copies)