Poll polymerase (DNA directed), lambda [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Pollprovided by MGI
Official Full Name: polymerase (DNA directed), lambdaprovided by MGI
Primary source: MGI:MGI:1889000
See related: Ensembl:ENSMUSG00000025218 AllianceGenome:MGI:1889000
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: 1110003P06Rik
Summary: Enables DNA-directed DNA polymerase activity. Predicted to be involved in DNA metabolic process. Predicted to be located in nucleoplasm. Predicted to be active in nucleus and site of double-strand break. Is expressed in muscle tissue; retina; and seminiferous tubule. Orthologous to human POLL (DNA polymerase lambda). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Ubiquitous expression in testis adult (RPKM 24.9), ovary adult (RPKM 5.9) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 19 C3; 19 38.75 cM

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	19	NC_000085.7 (45540714..45549025, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	19	NC_000085.6 (45552275..45560564, complement)

Chromosome 19 - NC_000085.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

DNA polymerases beta and lambda in single-nucleotide and multinucleotide pathways of mammalian base excision DNA repair
Title: Deployment of DNA polymerases beta and lambda in single-nucleotide and multinucleotide pathways of mammalian base excision DNA repair.
Pol mu and Pol lambda play a key role in conferring on NHEJ the flexibility required for accurate and efficient repair
Title: Essential role for polymerase specialization in cellular nonhomologous end joining.
Results show that deficiency of either DNA polymerases beta or lambda or both results in a modest but significant decrease in V region somatic hypermutation (SHM) with no effect on mutation specificity, suggesting no direct role in SHM.
Title: DNA polymerases β and λ do not directly affect Ig variable region somatic hypermutation although their absence reduces the frequency of mutations.
both pol lambda and pol beta interact with the upstream DNA glycosylases for repair of alkylated and oxidized DNA bases
Title: DNA polymerases beta and lambda mediate overlapping and independent roles in base excision repair in mouse embryonic fibroblasts.
analysis of the interaction between DNA Polymerase lambda and anticancer nucleoside analogs
Title: Interaction between DNA Polymerase lambda and anticancer nucleoside analogs.
Pol lambda contributes to base excision repair in mouse fibroblast cell extract.
Title: DNA polymerase lambda mediates a back-up base excision repair activity in extracts of mouse embryonic fibroblasts.
Pol lambda is not required for normal Ig gene hypermutation.
Title: Cutting edge: DNA polymerases mu and lambda are dispensable for Ig gene hypermutation.
Pol lambda protects cells against oxidative stress, and participates in oxidative DNA damage base excision repair.
Title: DNA polymerase lambda protects mouse fibroblasts against oxidative DNA damage and is recruited to sites of DNA damage/repair.
Hydrocephalus, situs inversus, chronic sinusitis, and male infertility in DNA polymerase lambda-deficient mice: possible implication for the pathogenesis of immotile cilia syndrome.
Title: Hydrocephalus, situs inversus, chronic sinusitis, and male infertility in DNA polymerase lambda-deficient mice: possible implication for the pathogenesis of immotile cilia syndrome.
third hypervariable region assumes for each immunoglobulin chain, with pol lambda maintaining a large heavy chain junctional heterogeneity and pol mu ensuring a restricted light chain junctional variability
Title: Nonoverlapping functions of DNA polymerases mu, lambda, and terminal deoxynucleotidyltransferase during immunoglobulin V(D)J recombination in vivo.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Targeted (1) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AV007317 (e-PCR)
- UniSTS:166157

Ms10l (e-PCR), detects polymorphism
- UniSTS:234001

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables 5'-deoxyribose-5-phosphate lyase activity	ISO Inferred from Sequence Orthology more info
enables 5'-deoxyribose-5-phosphate lyase activity	ISS Inferred from Sequence or Structural Similarity more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-directed DNA polymerase activity	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-directed DNA polymerase activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA-directed DNA polymerase activity	ISA Inferred from Sequence Alignment more info	PubMed
enables DNA-directed DNA polymerase activity	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in DNA biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in DNA replication	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within base-excision repair, gap-filling	ISA Inferred from Sequence Alignment more info	PubMed
involved_in base-excision repair, gap-filling	ISO Inferred from Sequence Orthology more info
involved_in base-excision repair, gap-filling	ISS Inferred from Sequence or Structural Similarity more info
involved_in double-strand break repair via homologous recombination	ISO Inferred from Sequence Orthology more info
involved_in double-strand break repair via homologous recombination	ISS Inferred from Sequence or Structural Similarity more info
involved_in double-strand break repair via nonhomologous end joining	IBA Inferred from Biological aspect of Ancestor more info
involved_in double-strand break repair via nonhomologous end joining	ISO Inferred from Sequence Orthology more info
involved_in double-strand break repair via nonhomologous end joining	ISS Inferred from Sequence or Structural Similarity more info
involved_in nucleotide-excision repair	ISO Inferred from Sequence Orthology more info
involved_in nucleotide-excision repair	ISS Inferred from Sequence or Structural Similarity more info	PubMed

Component	Evidence Code	Pubs
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
is_active_in site of double-strand break	ISO Inferred from Sequence Orthology more info
is_active_in site of double-strand break	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: DNA polymerase lambda

Names: pol Lambda

NP_001317435.1

EC 2.7.7.7

NP_001317436.1

EC 2.7.7.7

NP_064416.1

EC 2.7.7.7

XP_036017537.1

EC 2.7.7.7

XP_036017538.1

EC 2.7.7.7

XP_036017539.1

EC 2.7.7.7

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001330506.1 → NP_001317435.1 DNA polymerase lambda isoform 1

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same isoform (1).

Source sequence(s)

AC126454, AK003392, BE949923, BG080424, BY270661

Consensus CDS

CCDS29861.1

UniProtKB/Swiss-Prot

Q9CTJ1, Q9QXE2
NM_001330507.1 → NP_001317436.1 DNA polymerase lambda isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (3) uses an alternate splice site in the 3' coding region, compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1.

Source sequence(s)

AK003392, AK133087, BE949923, BG080424, BY270661, BY315476

UniProtKB/Swiss-Prot

Q9QXE2
NM_020032.3 → NP_064416.1 DNA polymerase lambda isoform 1

See identical proteins and their annotated locations for NP_064416.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the shortest transcript and encodes the longer isoform (1). Both variants 1 and 2 encode the same isoform (1).

Source sequence(s)

AK003392, BE949923, BG080424, BY270661, BY315476

Consensus CDS

CCDS29861.1

UniProtKB/Swiss-Prot

Q9CTJ1, Q9QXE2

Related

ENSMUSP00000026239.7, ENSMUST00000026239.7

Conserved Domains (3) summary

cd00141
Location:253 → 571

NT_POLXc; Nucleotidyltransferase (NT) domain of family X DNA Polymerases

smart00483
Location:249 → 572

POLXc; DNA polymerase X family

cd00027
Location:56 → 119

BRCT; Breast Cancer Suppressor Protein (BRCA1), carboxy-terminal domain. The BRCT domain is found within many DNA damage repair and cell cycle checkpoint proteins. The unique diversity of this domain superfamily allows BRCT modules to interact forming homo ...

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000085.7 Reference GRCm39 C57BL/6J

Range

45540714..45549025 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036161644.1 → XP_036017537.1 DNA polymerase lambda isoform X1

Conserved Domains (3) summary

cd00141
Location:253 → 580

NT_POLXc; Nucleotidyltransferase (NT) domain of family X DNA Polymerases

PHA03247
Location:134 → 231

PHA03247; large tegument protein UL36; Provisional

cd17715
Location:40 → 123

BRCT_polymerase_lambda; BRCT domain of DNA polymerase lambda and similar proteins
XM_036161646.1 → XP_036017539.1 DNA polymerase lambda isoform X1

Conserved Domains (3) summary

cd00141
Location:253 → 580

NT_POLXc; Nucleotidyltransferase (NT) domain of family X DNA Polymerases

PHA03247
Location:134 → 231

PHA03247; large tegument protein UL36; Provisional

cd17715
Location:40 → 123

BRCT_polymerase_lambda; BRCT domain of DNA polymerase lambda and similar proteins
XM_036161645.1 → XP_036017538.1 DNA polymerase lambda isoform X1

Conserved Domains (3) summary

cd00141
Location:253 → 580

NT_POLXc; Nucleotidyltransferase (NT) domain of family X DNA Polymerases

PHA03247
Location:134 → 231

PHA03247; large tegument protein UL36; Provisional

cd17715
Location:40 → 123

BRCT_polymerase_lambda; BRCT domain of DNA polymerase lambda and similar proteins