Ppp1r8 protein phosphatase 1, regulatory subunit 8 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ppp1r8provided by MGI
Official Full Name: protein phosphatase 1, regulatory subunit 8provided by MGI
Primary source: MGI:MGI:2140494
See related: Ensembl:ENSMUSG00000028882 AllianceGenome:MGI:2140494
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: NIPP1; 6330548N22Rik
Summary: Enables protein phosphatase regulator activity. Acts upstream of or within cell population proliferation. Predicted to be located in nucleoplasm. Predicted to be part of spliceosomal complex. Predicted to be active in nuclear speck. Is expressed in 1-cell stage embryo; 2-cell stage embryo; 4-cell stage embryo; adrenal gland; and brain. Orthologous to human PPP1R8 (protein phosphatase 1 regulatory subunit 8). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in CNS E11.5 (RPKM 36.1), CNS E14 (RPKM 26.4) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 4 D2.3; 4 65.97 cM

Exon count:: 7

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	4	NC_000070.7 (132554235..132570480, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	4	NC_000070.6 (132826924..132843169, complement)

Chromosome 4 - NC_000070.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Embryonic NIPP1 Depletion in Keratinocytes Triggers a Cell Cycle Arrest and Premature Senescence in Adult Mice.
Title: Embryonic NIPP1 Depletion in Keratinocytes Triggers a Cell Cycle Arrest and Premature Senescence in Adult Mice.
Nuclear Inhibitor of Protein Phosphatase 1 (NIPP1) Regulates CNS Tau Phosphorylation and Myelination During Development.
Title: Nuclear Inhibitor of Protein Phosphatase 1 (NIPP1) Regulates CNS Tau Phosphorylation and Myelination During Development.
Phosphatase Regulator NIPP1 Restrains Chemokine-Driven Skin Inflammation.
Title: Phosphatase Regulator NIPP1 Restrains Chemokine-Driven Skin Inflammation.
We conclude that NIPP1 is essential for mammalian spermatogenesis because it is indispensable for the proliferation and survival of progenitor germ cells, including (un)differentiated spermatogonia.
Title: The protein phosphatase 1 regulator NIPP1 is essential for mammalian spermatogenesis.
NIPP1 deletion abrogated PP1-mediated EZH2 dephosphorylation at two cyclin-dependent kinase sites (Thr-345/487), thereby generating hyperphosphorylated EZH2, which is a substrate for proteolytic degradation.
Title: The deletion of the protein phosphatase 1 regulator NIPP1 in testis causes hyperphosphorylation and degradation of the histone methyltransferase EZH2.
NIPP1 does not regulate the proliferation of hepatocytes but is a suppressor of biliary epithelial cell proliferation, including progenitor cells, in the adult liver.
Title: Brief Report: The Deletion of the Phosphatase Regulator NIPP1 Causes Progenitor Cell Expansion in the Adult Liver.
activation of the RAGE by advanced glycation end products or other ligands suppresses NIPP1 expression in diabetic nephropathy, contributes to podocyte hypertrophy, and glomerular inflammation
Title: Activation of the receptor for advanced glycation end products induces nuclear inhibitor of protein phosphatase-1 suppression.
Required for the global trimethylation of Histone 3 at Lysine 27 and is implicated in gene silencing by enhancer of zeste homolog (EZH)2.
Title: The transcriptional repressor NIPP1 is an essential player in EZH2-mediated gene silencing.
NIPP1(-/-) embryos showed severely retarded growth at embryonic day 6.5 (E6.5) and were resorbed by E8.5. This early embryonic lethality was not associated with increased apoptosis but correlated with impaired cell proliferation.
Title: The nuclear scaffold protein NIPP1 is essential for early embryonic development and cell proliferation.
NIPP1 has a role in the nuclear targeting and/or retention of PP1
Title: Interactor-mediated nuclear translocation and retention of protein phosphatase-1.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (2) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

NoName (e-PCR)
- UniSTS:225101

AU044684 (e-PCR)
- UniSTS:179080

Ppp1r8 (e-PCR), detects polymorphism
- UniSTS:462000

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables mRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables molecular function inhibitor activity	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein phosphatase inhibitor activity	IEA Inferred from Electronic Annotation more info
enables protein phosphatase regulator activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine phosphatase inhibitor activity	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in RNA splicing	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cell population proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mRNA processing	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in nuclear speck	IBA Inferred from Biological aspect of Ancestor more info
located_in nuclear speck	IEA Inferred from Electronic Annotation more info
located_in nuclear speck	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
part_of spliceosomal complex	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: nuclear inhibitor of protein phosphatase 1

Names: protein phosphatase 1, regulatory (inhibitor) subunit 8

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001290725.1 → NP_001277654.1 nuclear inhibitor of protein phosphatase 1 isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. It encodes isoform 2, which is shorter by an amino acid, compared to isoform 1.

Source sequence(s)

AK032022, BQ749177, BY257668, BY293277, CK625884

Consensus CDS

CCDS71482.1

UniProtKB/TrEMBL

A2ADR8

Related

ENSMUSP00000101539.2, ENSMUST00000105919.2

Conserved Domains (2) summary

cd00060
Location:27 → 119

FHA; Forkhead associated domain (FHA); found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain. FHA domains may bind phosphothreonine, phosphoserine and sometimes phosphotyrosine. In eukaryotes, many FHA domain-containing proteins ...

pfam11945
Location:98 → 212

WASH_WAHD; WAHD domain of WASH complex
NM_001355198.1 → NP_001342127.1 nuclear inhibitor of protein phosphatase 1 isoform 3

Status: VALIDATED

Source sequence(s)

AK032022, AL671190, BB855371, BQ749177, BY293277

Conserved Domains (2) summary

cd00060
Location:53 → 145

FHA; Forkhead associated domain (FHA); found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain. FHA domains may bind phosphothreonine, phosphoserine and sometimes phosphotyrosine. In eukaryotes, many FHA domain-containing proteins ...

pfam11945
Location:124 → 238

WASH_WAHD; WAHD domain of WASH complex
NM_001355199.1 → NP_001342128.1 nuclear inhibitor of protein phosphatase 1 isoform 4

Status: VALIDATED

Source sequence(s)

AK032022, AL671190, BB855371, BQ749177, BY293277

Conserved Domains (2) summary

cd00060
Location:53 → 145

FHA; Forkhead associated domain (FHA); found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain. FHA domains may bind phosphothreonine, phosphoserine and sometimes phosphotyrosine. In eukaryotes, many FHA domain-containing proteins ...

pfam11945
Location:124 → 238

WASH_WAHD; WAHD domain of WASH complex
NM_146154.3 → NP_666266.1 nuclear inhibitor of protein phosphatase 1 isoform 1

See identical proteins and their annotated locations for NP_666266.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes isoform 1.

Source sequence(s)

AK032022, BQ749177, BY293277

Consensus CDS

CCDS18736.1

UniProtKB/Swiss-Prot

Q8C087, Q8R3G1

Related

ENSMUSP00000030702.8, ENSMUST00000030702.14

Conserved Domains (2) summary

cd00060
Location:27 → 119

FHA; Forkhead associated domain (FHA); found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain. FHA domains may bind phosphothreonine, phosphoserine and sometimes phosphotyrosine. In eukaryotes, many FHA domain-containing proteins ...

pfam11945
Location:98 → 212

WASH_WAHD; WAHD domain of WASH complex