Shroom2 shroom family member 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Shroom2provided by MGI
Official Full Name: shroom family member 2provided by MGI
Primary source: MGI:MGI:107194
See related: Ensembl:ENSMUSG00000045180 AllianceGenome:MGI:107194
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Apxl; Shrm2; 4832440C16; C630003H05Rik
Summary: Enables actin filament binding activity; beta-catenin binding activity; and protein domain specific binding activity. Involved in cell migration and melanosome organization. Acts upstream of or within cell-cell junction maintenance and negative regulation of actin filament depolymerization. Located in several cellular components, including adherens junction; apical plasma membrane; and bicellular tight junction. Colocalizes with cortical actin cytoskeleton. Is expressed in several structures, including blood vessel; central nervous system; epithelium; inner ear; and retina inner plexiform layer. Orthologous to human SHROOM2 (shroom family member 2). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Broad expression in CNS E18 (RPKM 10.6), frontal lobe adult (RPKM 7.9) and 22 other tissues See more
Orthologs: human all
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Genomic context

Location:: X F3; X 68.46 cM

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	X	NC_000086.8 (151392505..151553784, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	X	NC_000086.7 (152609509..152770788, complement)

Chromosome X - NC_000086.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Data suggest that Shroom2 facilitates the formation of a contractile network within endothelial cells, the loss of which leads to an increase in endothelial sprouting, migration, and angiogenesis.
Title: Shroom2 regulates contractility to control endothelial morphogenesis.
Shroom2 and ZO-1 form a tight-junction-associated scaffolding complex, possibly linked to myosin VIIa, that bridges the junctional membrane to the underlying cytoskeleton, thereby contributing to the stabilization of these junctions.
Title: Shroom2, a myosin-VIIa- and actin-binding protein, directly interacts with ZO-1 at tight junctions.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Gene trapped (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables actin binding	IDA Inferred from Direct Assay more info	PubMed
enables actin filament binding	IBA Inferred from Biological aspect of Ancestor more info
enables actin filament binding	IDA Inferred from Direct Assay more info	PubMed
enables beta-catenin binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein domain specific binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
NOT involved_in actin filament bundle assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in actin filament organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in apical protein localization	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in brain development	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in camera-type eye development	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in camera-type eye morphogenesis	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
NOT involved_in cell morphogenesis	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within cell-cell junction maintenance	IDA Inferred from Direct Assay more info	PubMed
involved_in cellular pigment accumulation	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in ear development	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in establishment of melanosome localization	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in eye pigment granule organization	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in lens morphogenesis in camera-type eye	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in melanosome organization	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of actin filament depolymerization	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
is_active_in adherens junction	IBA Inferred from Biological aspect of Ancestor more info
located_in adherens junction	IDA Inferred from Direct Assay more info	PubMed
is_active_in apical junction complex	IBA Inferred from Biological aspect of Ancestor more info
located_in apical junction complex	IDA Inferred from Direct Assay more info	PubMed
is_active_in apical plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in apical plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in apical plasma membrane	ISS Inferred from Sequence or Structural Similarity more info	PubMed
located_in bicellular tight junction	IDA Inferred from Direct Assay more info	PubMed
located_in cell cortex	IDA Inferred from Direct Assay more info	PubMed
located_in cell-cell junction	IDA Inferred from Direct Assay more info	PubMed
colocalizes_with cortical actin cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
colocalizes_with cortical actin cytoskeleton	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
located_in microtubule	IEA Inferred from Electronic Annotation more info
located_in neuronal cell body	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: protein Shroom2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001290684.1 → NP_001277613.1 protein Shroom2 isoform 2

See identical proteins and their annotated locations for NP_001277613.1

Status: VALIDATED

Source sequence(s)

AL805960, AL807777

Consensus CDS

CCDS72455.1

UniProtKB/Swiss-Prot

A2ALU4, Q2EY23, Q8C7N1, Q8CCR2, Q8CDZ0

Related

ENSMUSP00000057500.5, ENSMUST00000062317.5

Conserved Domains (3) summary

cd00992
Location:24 → 105

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

pfam08687
Location:1186 → 1474

ASD2; Apx/Shroom domain ASD2

pfam08688
Location:662 → 836

ASD1; Apx/Shroom domain ASD1
NM_001290685.1 → NP_001277614.1 protein Shroom2 isoform 3 precursor

Status: VALIDATED

Source sequence(s)

AK049833, AK049850, AL805960, BC092373, BY125315

UniProtKB/Swiss-Prot

A2ALU4

UniProtKB/TrEMBL

Q8C7N5

Related

ENSMUST00000137217.8

Conserved Domains (1) summary

pfam08687
Location:166 → 455

ASD2; Apx/Shroom domain ASD2
NM_001290686.1 → NP_001277615.1 protein Shroom2 isoform 4

See identical proteins and their annotated locations for NP_001277615.1

Status: VALIDATED

Source sequence(s)

AK029338, AK049833, BY125315

UniProtKB/Swiss-Prot

A2ALU4

UniProtKB/TrEMBL

Q8C7N5

Conserved Domains (1) summary

pfam08687
Location:151 → 439

ASD2; Apx/Shroom domain ASD2
NM_001290687.2 → NP_001277616.1 protein Shroom2 isoform 4

See identical proteins and their annotated locations for NP_001277616.1

Status: VALIDATED

Source sequence(s)

AK049833, AK049850, AL805960, CF534250, CO431718

UniProtKB/Swiss-Prot

A2ALU4

UniProtKB/TrEMBL

Q8C7N5

Conserved Domains (1) summary

pfam08687
Location:151 → 439

ASD2; Apx/Shroom domain ASD2
NM_001359158.1 → NP_001346087.1 protein Shroom2 isoform 4

Status: VALIDATED

Source sequence(s)

AL805960

Conserved Domains (1) summary

pfam08687
Location:151 → 439

ASD2; Apx/Shroom domain ASD2
NM_001359161.1 → NP_001346090.1 protein Shroom2 isoform 4

Status: VALIDATED

Source sequence(s)

AL805960

Conserved Domains (1) summary

pfam08687
Location:151 → 439

ASD2; Apx/Shroom domain ASD2
NM_001359163.1 → NP_001346092.1 protein Shroom2 isoform 4

Status: VALIDATED

Source sequence(s)

AL805960

Conserved Domains (1) summary

pfam08687
Location:151 → 439

ASD2; Apx/Shroom domain ASD2
NM_172441.3 → NP_766029.2 protein Shroom2 isoform 1

See identical proteins and their annotated locations for NP_766029.2

Status: VALIDATED

Source sequence(s)

AK049833, AK049850, AL805960, AL807777, BF463332, CK635066

Consensus CDS

CCDS41179.1

UniProtKB/TrEMBL

A7TU71

Related

ENSMUSP00000098701.3, ENSMUST00000101141.9

Conserved Domains (3) summary

cd00992
Location:24 → 105

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

pfam08687
Location:1192 → 1480

ASD2; Apx/Shroom domain ASD2

pfam08688
Location:662 → 836

ASD1; Apx/Shroom domain ASD1

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000086.8 Reference GRCm39 C57BL/6J

Range

151392505..151553784 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006528683.4 → XP_006528746.1 protein Shroom2 isoform X1

Conserved Domains (3) summary

cd00992
Location:24 → 105

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

pfam08687
Location:1187 → 1475

ASD2; Apx/Shroom domain ASD2

pfam08688
Location:662 → 836

ASD1; Apx/Shroom domain ASD1
XM_011247774.4 → XP_011246076.1 protein Shroom2 isoform X2

See identical proteins and their annotated locations for XP_011246076.1

UniProtKB/Swiss-Prot

A2ALU4

Conserved Domains (1) summary

pfam08687
Location:151 → 439

ASD2; Apx/Shroom domain ASD2
XM_011247775.4 → XP_011246077.1 protein Shroom2 isoform X2

See identical proteins and their annotated locations for XP_011246077.1

UniProtKB/Swiss-Prot

A2ALU4

Conserved Domains (1) summary

pfam08687
Location:151 → 439

ASD2; Apx/Shroom domain ASD2
XM_011247777.4 → XP_011246079.1 protein Shroom2 isoform X2

See identical proteins and their annotated locations for XP_011246079.1

UniProtKB/Swiss-Prot

A2ALU4

Conserved Domains (1) summary

pfam08687
Location:151 → 439

ASD2; Apx/Shroom domain ASD2