Raf1 v-raf-leukemia viral oncogene 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Raf1provided by MGI
Official Full Name: v-raf-leukemia viral oncogene 1provided by MGI
Primary source: MGI:MGI:97847
See related: Ensembl:ENSMUSG00000000441 AllianceGenome:MGI:97847
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: cRaf; Craf1; Raf-1; c-Raf; v-Raf; 6430402F14Rik; D830050J10Rik
Summary: Enables MAP kinase kinase kinase activity and small GTPase binding activity. Involved in cell surface receptor protein tyrosine kinase signaling pathway and nervous system development. Acts upstream of with a positive effect on type B pancreatic cell proliferation. Acts upstream of or within several processes, including cell surface receptor signaling pathway; insulin secretion involved in cellular response to glucose stimulus; and positive regulation of macromolecule metabolic process. Located in pseudopodium. Is expressed in several structures, including alimentary system; central nervous system; early conceptus; genitourinary system; and immune system. Used to study Noonan syndrome 5. Human ortholog(s) of this gene implicated in Noonan syndrome (multiple); Noonan syndrome with multiple lentigines 2; adenocarcinoma (multiple); and dilated cardiomyopathy 1NN. Orthologous to human RAF1 (Raf-1 proto-oncogene, serine/threonine kinase). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Ubiquitous expression in CNS E14 (RPKM 50.6), whole brain E14.5 (RPKM 48.5) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 6 E3; 6 53.62 cM

Exon count:: 19

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	6	NC_000072.7 (115595530..115653596, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	6	NC_000072.6 (115618569..115676635, complement)

Chromosome 6 - NC_000072.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Taurine promotes insulin synthesis by enhancing Isl-1 expression through miR-7a/RAF1/ERK1/2 pathway.
Title: Taurine promotes insulin synthesis by enhancing Isl-1 expression through miR-7a/RAF1/ERK1/2 pathway.
Sex disparities in non-small cell lung cancer: mechanistic insights from a cRaf transgenic disease model.
Title: Sex disparities in non-small cell lung cancer: mechanistic insights from a cRaf transgenic disease model.
SHOC2 mediates the drug-resistance of triple-negative breast cancer cells to everolimus.
Title: SHOC2 mediates the drug-resistance of triple-negative breast cancer cells to everolimus.
MicroRNA-7a inhibits Isl1 expression to regulate insulin secretion by targeting Raf1 and Mapkap1 in NIT-1 cells.
Title: MicroRNA-7a inhibits Isl1 expression to regulate insulin secretion by targeting Raf1 and Mapkap1 in NIT-1 cells.
Protective Effect of Raf-1 Kinase Inhibitory Protein on Diabetic Retinal Neurodegeneration through P38-MAPK Pathway.
Title: Protective Effect of Raf-1 Kinase Inhibitory Protein on Diabetic Retinal Neurodegeneration through P38-MAPK Pathway.
Inhibition of ubiquitin-specific protease 13-mediated degradation of Raf1 kinase by Spautin-1 has opposing effects in naive and primed pluripotent stem cells.
Title: Inhibition of ubiquitin-specific protease 13-mediated degradation of Raf1 kinase by Spautin-1 has opposing effects in naïve and primed pluripotent stem cells.
The role of miR-7 as a potential switch in the mouse hypothalamus-pituitary-ovary axis through regulation of gonadotropins.
Title: The role of miR-7 as a potential switch in the mouse hypothalamus-pituitary-ovary axis through regulation of gonadotropins.
Early But Not Delayed Optogenetic RAF Activation Promotes Astrocytogenesis in Mouse Neural Progenitors.
Title: Early But Not Delayed Optogenetic RAF Activation Promotes Astrocytogenesis in Mouse Neural Progenitors.
Tumor regression and resistance mechanisms upon CDK4 and RAF1 inactivation in KRAS/P53 mutant lung adenocarcinomas.
Title: Tumor regression and resistance mechanisms upon CDK4 and RAF1 inactivation in KRAS/P53 mutant lung adenocarcinomas.
A CRAF/glutathione-S-transferase P1 complex sustains autocrine growth of cancers with KRAS and BRAF mutations.
Title: A CRAF/glutathione-S-transferase P1 complex sustains autocrine growth of cancers with KRAS and BRAF mutations.

Submit: New GeneRIF Correction See all GeneRIFs (93)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (4)
Targeted (15) 1 citation
Transgenic (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

UniSTS:469819 (e-PCR)
- UniSTS:469819

Raf1 (e-PCR), detects polymorphism
- UniSTS:469819

BB129353 (e-PCR)
- UniSTS:185679

RH127069 (e-PCR)
- UniSTS:208014

AA990557 (e-PCR)
- UniSTS:160660

PMC125416P1 (e-PCR)
- UniSTS:270498

Raf1 (e-PCR), detects polymorphism
- UniSTS:536493

Raf1 (e-PCR), detects polymorphism
- UniSTS:259519

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables 3-phosphoinositide-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables AMP-activated protein kinase activity	IEA Inferred from Electronic Annotation more info
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP binding	ISO Inferred from Sequence Orthology more info
enables DNA-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables MAP kinase kinase kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables MAP kinase kinase kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables MAP kinase kinase kinase activity	ISO Inferred from Sequence Orthology more info
enables Rho-dependent protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables adenylate cyclase activator activity	ISO Inferred from Sequence Orthology more info
enables adenylate cyclase binding	ISO Inferred from Sequence Orthology more info
enables enzyme binding	ISO Inferred from Sequence Orthology more info
enables eukaryotic translation initiation factor 2alpha kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AS1 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AS121 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AT120 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AXS139 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS14 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS36 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S10 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S28 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S57 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T11 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T3 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T45 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T6 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H4S1 kinase activity	IEA Inferred from Electronic Annotation more info
enables identical protein binding	IEA Inferred from Electronic Annotation more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables mitogen-activated protein kinase kinase binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	ISO Inferred from Sequence Orthology more info
enables protein kinase activity	TAS Traceable Author Statement more info	PubMed
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info	PubMed
enables protein-containing complex binding	ISO Inferred from Sequence Orthology more info
enables ribosomal protein S6 kinase activity	IEA Inferred from Electronic Annotation more info
enables small GTPase binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in ERBB2-ERBB3 signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in MAPK cascade	IBA Inferred from Biological aspect of Ancestor more info
involved_in MAPK cascade	ISO Inferred from Sequence Orthology more info	PubMed
involved_in Schwann cell development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in activation of adenylate cyclase activity	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cell differentiation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within death-inducing signaling complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of extrinsic apoptotic signaling pathway via death domain receptors	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of extrinsic apoptotic signaling pathway via death domain receptors	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within face development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in insulin receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within insulin secretion involved in cellular response to glucose stimulus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in insulin-like growth factor receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in insulin-like growth factor receptor signaling pathway	ISO Inferred from Sequence Orthology more info	PubMed
acts_upstream_of_or_within intermediate filament cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within intracellular glucose homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within intracellular signal transduction	TAS Traceable Author Statement more info	PubMed
involved_in myelination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cell population proliferation	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of cell population proliferation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of extrinsic apoptotic signaling pathway via death domain receptors	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within negative regulation of extrinsic apoptotic signaling pathway via death domain receptors	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of protein-containing complex assembly	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of protein-containing complex assembly	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within neurotrophin TRK receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of MAPK cascade	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of peptidyl-serine phosphorylation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of protein phosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to hypoxia	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within response to muscle stretch	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within somatic stem cell population maintenance	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within thymus development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within thyroid gland development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_positive_effect type B pancreatic cell proliferation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi apparatus	ISO Inferred from Sequence Orthology more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info	PubMed
located_in cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in cytosol	TAS Traceable Author Statement more info	PubMed
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrion	IEA Inferred from Electronic Annotation more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
colocalizes_with plasma membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info	PubMed
located_in plasma membrane	ISS Inferred from Sequence or Structural Similarity more info
located_in plasma membrane	TAS Traceable Author Statement more info	PubMed
located_in pseudopodium	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: RAF proto-oncogene serine/threonine-protein kinase

Names: Craf1 transforming; murine sarcoma 3611 oncogene 1; proto-oncogene c-RAF

NP_001343262.1

EC 2.7.11.1

NP_001343263.1

EC 2.7.11.1

NP_084056.1

EC 2.7.11.1

XP_006505427.1

EC 2.7.11.1

XP_030110958.1

EC 2.7.11.1

XP_030110959.1

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001356333.2 → NP_001343262.1 RAF proto-oncogene serine/threonine-protein kinase isoform 2

Status: VALIDATED

Source sequence(s)

AC160032

Conserved Domains (3) summary

cd14149
Location:356 → 638

STKc_C-Raf; Catalytic domain of the Serine/Threonine Kinase, C-Raf (Rapidly Accelerated Fibrosarcoma) kinase

cd17135
Location:55 → 131

RBD_CRAF; Ras-binding domain (RBD) found in RAF proto-oncogene serine/threonine-protein kinase RAF1/CRAF

pfam00130
Location:139 → 184

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
NM_001356334.2 → NP_001343263.1 RAF proto-oncogene serine/threonine-protein kinase isoform 1

Status: VALIDATED

Source sequence(s)

AC160032

Consensus CDS

CCDS20441.1

UniProtKB/Swiss-Prot

Q3UR68, Q58E75, Q91WH1, Q99N57, Q99N58, Q9QUU8

Related

ENSMUSP00000108571.2, ENSMUST00000112949.8

Conserved Domains (3) summary

cd01816
Location:57 → 131

Raf_RBD; Ubiquitin domain of Raf serine/threonine kinases

cd14149
Location:336 → 618

STKc_C-Raf; Catalytic domain of the Serine/Threonine Kinase, C-Raf (Rapidly Accelerated Fibrosarcoma) kinase

pfam00130
Location:139 → 187

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
NM_029780.4 → NP_084056.1 RAF proto-oncogene serine/threonine-protein kinase isoform 1

See identical proteins and their annotated locations for NP_084056.1

Status: VALIDATED

Source sequence(s)

AK141745, BF455519

Consensus CDS

CCDS20441.1

UniProtKB/Swiss-Prot

Q3UR68, Q58E75, Q91WH1, Q99N57, Q99N58, Q9QUU8

Related

ENSMUSP00000000451.8, ENSMUST00000000451.14

Conserved Domains (3) summary

cd01816
Location:57 → 131

Raf_RBD; Ubiquitin domain of Raf serine/threonine kinases

cd14149
Location:336 → 618

STKc_C-Raf; Catalytic domain of the Serine/Threonine Kinase, C-Raf (Rapidly Accelerated Fibrosarcoma) kinase

pfam00130
Location:139 → 187

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000072.7 Reference GRCm39 C57BL/6J

Range

115595530..115653596 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006505364.3 → XP_006505427.1 RAF proto-oncogene serine/threonine-protein kinase isoform X1

See identical proteins and their annotated locations for XP_006505427.1

UniProtKB/Swiss-Prot

Q99N57

Conserved Domains (3) summary

cd14149
Location:356 → 638

STKc_C-Raf; Catalytic domain of the Serine/Threonine Kinase, C-Raf (Rapidly Accelerated Fibrosarcoma) kinase

cd17135
Location:55 → 131

RBD_CRAF; Ras-binding domain (RBD) found in RAF proto-oncogene serine/threonine-protein kinase RAF1/CRAF

pfam00130
Location:139 → 184

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
XM_030255099.1 → XP_030110959.1 RAF proto-oncogene serine/threonine-protein kinase isoform X3

Conserved Domains (3) summary

cd14149
Location:255 → 537

STKc_C-Raf; Catalytic domain of the Serine/Threonine Kinase, C-Raf (Rapidly Accelerated Fibrosarcoma) kinase

pfam00130
Location:58 → 103

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

cl28922
Location:27 → 50

Ubiquitin_like_fold; Beta-grasp ubiquitin-like fold
XM_030255098.1 → XP_030110958.1 RAF proto-oncogene serine/threonine-protein kinase isoform X2

Conserved Domains (2) summary

cd14149
Location:275 → 557

STKc_C-Raf; Catalytic domain of the Serine/Threonine Kinase, C-Raf (Rapidly Accelerated Fibrosarcoma) kinase

pfam00130
Location:58 → 103

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)