Amph amphiphysin [Rattus norvegicus (Norway rat)] - Gene

Summary

Symbol: Amphprovided by RGD
Full Name: amphiphysinprovided by RGD
Primary source: RGD:620274
See related: EnsemblRapid:ENSRNOG00000059510 AllianceGenome:RGD:620274
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Amph1
Summary: Enables phosphatase binding activity. Involved in positive regulation of GTPase activity and positive regulation of endocytosis. Located in axon terminus. Is active in postsynaptic endocytic zone. Is extrinsic component of synaptic vesicle membrane. Orthologous to human AMPH (amphiphysin). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in Brain (RPKM 444.2), Lung (RPKM 72.5) and 5 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 17q11

Exon count:: 24

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	17	NC_086035.1 (50435018..50678583, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	17	NC_051352.1 (45739385..45982905, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	17	NC_005116.4 (48304324..48562838, complement)

Chromosome 17 - NC_086035.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

amphiphysin interaction with N-WASP regulates actin assembly
Title: Dynamic interaction of amphiphysin with N-WASP regulates actin assembly.
cooperative function of dynamin 2 and amphiphysin 1 in phosphatidylserine-dependent phagocytosis is strongly suggested in sertoli cells
Title: Dynamin 2 cooperates with amphiphysin 1 in phagocytosis in sertoli cells.
there are two sets of amphI phosphosites that are either dynamically turning over or constitutively phosphorylated in nerve terminals
Title: The in vivo phosphorylation sites in multiple isoforms of amphiphysin I from rat brain nerve terminals.
amphiphysin 1 is important in the regulation of actin dynamics
Title: Amphiphysin 1 is important for actin polymerization during phagocytosis.
MAPK phosphorylation of amphiphysin1 controls NGF receptor/TrkA-mediated endocytosis by terminating the amphiphysin1-AP-2 interaction
Title: Regulation of amphiphysin1 by mitogen-activated protein kinase: its significance in nerve growth factor receptor-mediated endocytosis.
Endocytosis of the synaptic vesicle membrane in retinal ribbon synapses proceeds through a pathway similar to the one that is used in conventional synapses, although amphiphysin Ir is substituted for amphiphysin I.
Title: Localized expression of amphiphysin Ir, a retina-specific variant of amphiphysin I, in the ribbon synapse and its functional implication.
first demonstration of the co-expression of all three dynamin isoforms in a non-tumor cell
Title: Expression of the endocytic proteins dynamin and amphiphysin in rat gastric enterochromaffin-like cells.
A significant decrease in the levels of amphiphysin 1 mRNA is found in the hippocampus of aged rats subjected to repeated variable stress compared with treated and untreated adult rats or to untreated aged rats.
Title: Reduction in levels of amphiphysin 1 mRNA in the hippocampus of aged rats subjected to repeated variable stress.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables phosphatase binding	IPI Inferred from Physical Interaction more info	PubMed
enables phospholipid binding	IBA Inferred from Biological aspect of Ancestor more info
enables phospholipid binding	IEA Inferred from Electronic Annotation more info
enables phospholipid binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-containing complex binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within learning	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of GTPase activity	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in synaptic vesicle endocytosis	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within synaptic vesicle endocytosis	ISO Inferred from Sequence Orthology more info
involved_in synaptic vesicle endocytosis	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in axon terminus	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
is_active_in extrinsic component of synaptic vesicle membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	ISO Inferred from Sequence Orthology more info
located_in leading edge membrane	IEA Inferred from Electronic Annotation more info
located_in leading edge membrane	ISO Inferred from Sequence Orthology more info
is_active_in photoreceptor ribbon synapse	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
is_active_in postsynaptic endocytic zone	IDA Inferred from Direct Assay more info	PubMed
is_active_in postsynaptic endocytic zone	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in presynapse	ISO Inferred from Sequence Orthology more info
is_active_in presynaptic endocytic zone	ISO Inferred from Sequence Orthology more info
located_in synapse	IEA Inferred from Electronic Annotation more info
is_active_in synaptic vesicle	IBA Inferred from Biological aspect of Ancestor more info
located_in synaptic vesicle	ISO Inferred from Sequence Orthology more info
located_in terminal bouton	HDA more info	PubMed

General protein information

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Preferred Names: amphiphysin

Names: amphiphysin 1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_022217.2 → NP_071553.2 amphiphysin

Status: VALIDATED

Source sequence(s)

JAXUCZ010000017

Related

ENSRNOP00000073615.3, ENSRNOT00000080800.3

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086035.1 Reference GRCr8

Range

50435018..50678583 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039096041.2 → XP_038951969.1 amphiphysin isoform X2

UniProtKB/Swiss-Prot

O08838

Conserved Domains (3) summary

cd07611
Location:26 → 236

BAR_Amphiphysin_I_II; The Bin/Amphiphysin/Rvs (BAR) domain of Amphiphysin I and II

PHA02682
Location:248 → 357

PHA02682; ORF080 virion core protein; Provisional

cd12140
Location:570 → 640

SH3_Amphiphysin_I; Src Homology 3 domain of Amphiphysin I
XM_063276719.1 → XP_063132789.1 amphiphysin isoform X4

Related

ENSRNOP00000070116.2, ENSRNOT00000084702.3
XM_039096042.2 → XP_038951970.1 amphiphysin isoform X3

UniProtKB/Swiss-Prot

O08838

Conserved Domains (3) summary

PHA02682
Location:179 → 288

PHA02682; ORF080 virion core protein; Provisional

cd12140
Location:542 → 612

SH3_Amphiphysin_I; Src Homology 3 domain of Amphiphysin I

cl12013
Location:1 → 167

BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
XM_039096040.2 → XP_038951968.1 amphiphysin isoform X1

UniProtKB/Swiss-Prot

O08838

UniProtKB/TrEMBL

A0A0G2K5Z4

Related

ENSRNOP00000017102.8, ENSRNOT00000017102.8

Conserved Domains (3) summary

cd07611
Location:23 → 233

BAR_Amphiphysin_I_II; The Bin/Amphiphysin/Rvs (BAR) domain of Amphiphysin I and II

PHA02682
Location:245 → 354

PHA02682; ORF080 virion core protein; Provisional

cd12140
Location:608 → 678

SH3_Amphiphysin_I; Src Homology 3 domain of Amphiphysin I