Mid2 midline 2 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Mid2provided by RGD
Official Full Name: midline 2provided by RGD
Primary source: RGD:1565674
See related: EnsemblRapid:ENSRNOG00000060542 AllianceGenome:RGD:1565674
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: RGD1565674
Summary: Predicted to enable several functions, including microtubule binding activity; protein homodimerization activity; and transcription coactivator activity. Predicted to be involved in several processes, including defense response to symbiont; negative regulation of viral transcription; and positive regulation of NF-kappaB transcription factor activity. Predicted to be located in cytoplasm. Predicted to colocalize with microtubule. Human ortholog(s) of this gene implicated in non-syndromic X-linked intellectual disability 101. Orthologous to human MID2 (midline 2). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Lung (RPKM 111.1), Adrenal (RPKM 61.7) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: Xq33

Exon count:: 11

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	X	NC_086039.1 (109143057..109245257)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	X	NC_051356.1 (104354692..104456757)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	X	NC_005120.4 (112019897..112121980)

Chromosome X - NC_086039.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

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General gene information

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Markers

Mid2 (e-PCR), detects polymorphism
- UniSTS:546866

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables enzyme binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables microtubule binding	IBA Inferred from Biological aspect of Ancestor more info
enables microtubule binding	ISO Inferred from Sequence Orthology more info
enables phosphoprotein binding	ISO Inferred from Sequence Orthology more info
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info
enables transcription coactivator activity	ISO Inferred from Sequence Orthology more info
enables transferase activity	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in innate immune response	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of viral entry into host cell	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of viral transcription	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of DNA-binding transcription factor activity	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of NF-kappaB transcription factor activity	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of autophagy	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of canonical NF-kappaB signal transduction	ISO Inferred from Sequence Orthology more info
involved_in protein localization to microtubule	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein localization to microtubule	ISO Inferred from Sequence Orthology more info
involved_in suppression of viral release by host	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
colocalizes_with microtubule	IBA Inferred from Biological aspect of Ancestor more info
located_in microtubule	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: probable E3 ubiquitin-protein ligase MID2

NP_001178818.1

EC 2.3.2.27

XP_006234309.1

EC 2.3.2.27

XP_006234310.1

EC 2.3.2.27

XP_063136223.1

EC 2.3.2.27

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001191889.2 → NP_001178818.1 probable E3 ubiquitin-protein ligase MID2 isoform 3

Status: VALIDATED

Source sequence(s)

JAXUCZ010000021

UniProtKB/TrEMBL

A0A0G2JWJ3

Related

ENSRNOP00000069903.2, ENSRNOT00000079841.3

Conserved Domains (5) summary

smart00502
Location:239 → 364

BBC; B-Box C-terminal domain

smart00336
Location:195 → 232

BBOX; B-Box-type zinc finger

cd00063
Location:401 → 531

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

cd00162
Location:29 → 83

RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...

cd13739
Location:536 → 705

SPRY_PRY_TRIM1; PRY/SPRY domain of tripartite motif-binding protein 1 (TRIM1) or MID2
NM_001434288.1 → NP_001421217.1 probable E3 ubiquitin-protein ligase MID2 isoform 1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000021

UniProtKB/TrEMBL

A6HLR4
NM_001434289.1 → NP_001421218.1 probable E3 ubiquitin-protein ligase MID2 isoform 2

Status: VALIDATED

Source sequence(s)

JAXUCZ010000021
NM_001434290.1 → NP_001421219.1 probable E3 ubiquitin-protein ligase MID2 isoform 4

Status: VALIDATED

Source sequence(s)

JAXUCZ010000021

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086039.1 Reference GRCr8

Range

109143057..109245257

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_063280153.1 → XP_063136223.1 probable E3 ubiquitin-protein ligase MID2 isoform X2

Related

ENSRNOP00000101518.1, ENSRNOT00000118902.2
XM_006234248.5 → XP_006234310.1 probable E3 ubiquitin-protein ligase MID2 isoform X3

Conserved Domains (5) summary

smart00502
Location:219 → 344

BBC; B-Box C-terminal domain

smart00336
Location:175 → 212

BBOX; B-Box-type zinc finger

cd00063
Location:381 → 511

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

cd00162
Location:9 → 63

RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...

cd13739
Location:516 → 685

SPRY_PRY_TRIM1; PRY/SPRY domain of tripartite motif-binding protein 1 (TRIM1) or MID2
XM_006234247.5 → XP_006234309.1 probable E3 ubiquitin-protein ligase MID2 isoform X1

Conserved Domains (5) summary

smart00502
Location:239 → 364

BBC; B-Box C-terminal domain

smart00336
Location:195 → 232

BBOX; B-Box-type zinc finger

cd00063
Location:401 → 501

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

cd00162
Location:29 → 83

RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...

cd13739
Location:506 → 675

SPRY_PRY_TRIM1; PRY/SPRY domain of tripartite motif-binding protein 1 (TRIM1) or MID2