Prkar2b protein kinase cAMP-dependent type II regulatory subunit beta [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Prkar2bprovided by RGD
Official Full Name: protein kinase cAMP-dependent type II regulatory subunit betaprovided by RGD
Primary source: RGD:3394
See related: EnsemblRapid:ENSRNOG00000009079 AllianceGenome:RGD:3394
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: RATDNA
Summary: Enables cAMP binding activity; cAMP-dependent protein kinase regulator activity; and protein domain specific binding activity. Involved in regulation of protein kinase activity and response to antipsychotic drug. Located in dendrite; neuronal cell body; and perinuclear region of cytoplasm. Part of cAMP-dependent protein kinase complex. Orthologous to human PRKAR2B (protein kinase cAMP-dependent type II regulatory subunit beta). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Thymus (RPKM 683.9), Adrenal (RPKM 590.0) and 7 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 6q16

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	6	NC_086024.1 (54291143..54381639, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	6	NC_051341.1 (48563659..48653933, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	6	NC_005105.4 (51265509..51356383, complement)

Chromosome 6 - NC_086024.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Release of pRIIbeta in the presence of cAMP is reduced by calcium, whereas autophosphorylation at the phosphorylation site inhibits holoenzyme reassociation with the catalytic subunit.
Title: Single Turnover Autophosphorylation Cycle of the PKA RIIβ Holoenzyme.
The unique structural properties of RiIbeta do not require the C-terminal cAMP-binding domain.
Title: The roles of the RIIβ linker and N-terminal cyclic nucleotide-binding domain in determining the unique structures of the type IIβ protein kinase A: a small angle x-ray and neutron scattering study.
study describes the 2.3 angstrom structure of full-length tetrameric RIIbeta(2):C(2) holoenzyme
Title: Structure and allostery of the PKA RIIβ tetrameric holoenzyme.
The novel orientation of the linker peptide provides the first clues as to how this region contributes to the unique organization of the RIIbeta holoenzyme.
Title: Novel isoform-specific interfaces revealed by PKA RIIbeta holoenzyme structures.
InsP3R1 forms a complex with AKAP450 and PKAIIbeta, localized in the Golgi apparatus of cerebellar granule cells.
Title: Ins(1,4,5)P3 receptor type 1 associates with AKAP9 (AKAP450 variant) and protein kinase A type IIbeta in the Golgi apparatus in cerebellar granule cells.
Direct evidence is provided that the type II beta protein kinase A regulatory subunit may be partially associated with the type I alpha catalytic subunit with the pseudosubstrate sequence docked in the active site cleft in the presence of cAMP.
Title: Probing cAMP-dependent protein kinase holoenzyme complexes I alpha and II beta by FT-IR and chemical protein footprinting.
the B domain of type II beta regulatory subunit of cAMP-dependent protein kinase inhibits holoenzyme activation for RIIbeta
Title: cAMP-dependent protein kinase regulatory subunit type IIbeta: active site mutations define an isoform-specific network for allosteric signaling by cAMP.

Submit: New GeneRIF Correction

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH139912 (e-PCR)
- UniSTS:221996

d6arb12 (e-PCR)
- UniSTS:122176

RH140571 (e-PCR)
- UniSTS:222640

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables cAMP binding	IBA Inferred from Biological aspect of Ancestor more info
enables cAMP binding	IDA Inferred from Direct Assay more info	PubMed
enables cAMP binding	IEA Inferred from Electronic Annotation more info
enables cAMP-dependent protein kinase inhibitor activity	IBA Inferred from Biological aspect of Ancestor more info
enables cAMP-dependent protein kinase inhibitor activity	ISO Inferred from Sequence Orthology more info
enables cAMP-dependent protein kinase regulator activity	IDA Inferred from Direct Assay more info	PubMed
enables cAMP-dependent protein kinase regulator activity	IEA Inferred from Electronic Annotation more info
enables cAMP-dependent protein kinase regulator activity	ISO Inferred from Sequence Orthology more info
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein domain specific binding	IDA Inferred from Direct Assay more info	PubMed
enables protein kinase A catalytic subunit binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein kinase A catalytic subunit binding	IC Inferred by Curator more info	PubMed
enables protein kinase A catalytic subunit binding	ISO Inferred from Sequence Orthology more info
enables protein kinase binding	ISO Inferred from Sequence Orthology more info
enables ubiquitin protein ligase binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in adenylate cyclase-activating G protein-coupled receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within fatty acid metabolic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within learning	ISO Inferred from Sequence Orthology more info
involved_in modulation of chemical synaptic transmission	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cAMP/PKA signal transduction	ISO Inferred from Sequence Orthology more info
involved_in regulation of protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
involved_in response to antipsychotic drug	IEP Inferred from Expression Pattern more info	PubMed

Component	Evidence Code	Pubs
part_of cAMP-dependent protein kinase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of cAMP-dependent protein kinase complex	IDA Inferred from Direct Assay more info	PubMed
part_of cAMP-dependent protein kinase complex	IEA Inferred from Electronic Annotation more info
part_of cAMP-dependent protein kinase complex	ISO Inferred from Sequence Orthology more info
located_in centrosome	ISO Inferred from Sequence Orthology more info
located_in ciliary base	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in dendrite	IDA Inferred from Direct Assay more info	PubMed
located_in dendritic shaft	ISO Inferred from Sequence Orthology more info
located_in dendritic shaft	ISS Inferred from Sequence or Structural Similarity more info
located_in dendritic spine	ISO Inferred from Sequence Orthology more info
located_in dendritic spine	ISS Inferred from Sequence or Structural Similarity more info
is_active_in glutamatergic synapse	ISO Inferred from Sequence Orthology more info
located_in neuronal cell body	IDA Inferred from Direct Assay more info	PubMed
located_in perinuclear region of cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in postsynapse	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: cAMP-dependent protein kinase type II-beta regulatory subunit

Names: Type II beta regulatory subunit of cAMP-dependent protein kinase; protein kinase cAMP-dependent type 2 regulatory subunit beta; protein kinase, cAMP dependent regulatory, type II beta; protein kinase, cAMP-dependent, regulatory subunit type II beta

NP_001025191.1

EC 2.7.11.1

XP_017449526.3

EC 2.7.11.1

XP_063117623.1

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001030020.1 → NP_001025191.1 cAMP-dependent protein kinase type II-beta regulatory subunit

See identical proteins and their annotated locations for NP_001025191.1

Status: VALIDATED

Source sequence(s)

BI298837, CB814252, CX569141, M12492

UniProtKB/Swiss-Prot

P12369

UniProtKB/TrEMBL

A6HB59

Related

ENSRNOP00000012415.6, ENSRNOT00000012415.8

Conserved Domains (2) summary

cd12104
Location:3 → 43

DD_RIIbeta_PKA; Dimerization/Docking domain of the Type II beta Regulatory subunit of cAMP-dependent protein kinase

cd00038
Location:274 → 394

CAP_ED; effector domain of the CAP family of transcription factors; members include CAP (or cAMP receptor protein (CRP)), which binds cAMP, FNR (fumarate and nitrate reduction), which uses an iron-sulfur cluster to sense oxygen) and CooA, a heme containing CO ...

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086024.1 Reference GRCr8

Range

54291143..54381639 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_017594037.3 → XP_017449526.3 cAMP-dependent protein kinase type II-beta regulatory subunit isoform X1

UniProtKB/TrEMBL

Q4KLG5

Related

ENSRNOP00000074658.1, ENSRNOT00000079719.3
XM_063261553.1 → XP_063117623.1 cAMP-dependent protein kinase type II-beta regulatory subunit isoform X2