Ppp1r1b protein phosphatase 1, regulatory (inhibitor) subunit 1B [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Ppp1r1bprovided by RGD
Official Full Name: protein phosphatase 1, regulatory (inhibitor) subunit 1Bprovided by RGD
Primary source: RGD:621859
See related: EnsemblRapid:ENSRNOG00000028404 AllianceGenome:RGD:621859
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Darpp32; Darpp-32
Summary: Enables dopamine receptor binding activity. Involved in several processes, including memory; response to cocaine; and response to nicotine. Located in dendritic spine head and dendritic spine neck. Is active in glutamatergic synapse and postsynapse. Used to study depressive disorder; schizophrenia; status epilepticus; and withdrawal disorder. Biomarker of Huntington's disease and brain infarction. Human ortholog(s) of this gene implicated in bipolar disorder and schizophrenia. Orthologous to human PPP1R1B (protein phosphatase 1 regulatory inhibitor subunit 1B). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Brain (RPKM 199.2), Kidney (RPKM 43.7) and 2 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 10q31

Exon count:: 7

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	10	NC_086028.1 (83843948..83853063)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	10	NC_051345.1 (83347731..83356775)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	10	NC_005109.4 (86303727..86312770)

Chromosome 10 - NC_086028.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Epistatic evidence for gender-dependant slow neurotransmission signalling in substance use disorders: PPP1R12B versus PPP1R1B.
Title: Epistatic evidence for gender-dependant slow neurotransmission signalling in substance use disorders: PPP1R12B versus PPP1R1B.
The study measured DARPP32 and pThr34 DARPP32 in the brains of male Long-Evans rats with a history of sucrose self-administration followed by 1 or 30 days of abstinence and exposure to either overnight (acute) or one month (chronic) environmental enrichment (EE). Both acute and chronic EE reduced DARPP32 in the nucleus accumbens core and acute EE increased the ratio of phosphorylated to total DARPP32.
Title: Sucrose Abstinence and Environmental Enrichment Effects on Mesocorticolimbic DARPP32 in Rats.
Results indicate that the M4 muscarinic receptor plays a critical role in modulating phosphorylation of DARPP-32-Thr75 in striatal medium spiny neurons; suggest that M4 receptor activation acts antagonistically with dopamine D1-like receptors within the striatum, and indicate that M4 receptors may be a potential target for the treatment of Parkinson's disease and other relevant central nervous system disorders.
Title: Muscarinic acetylcholine M(4) receptors play a critical role in oxotremorine-induced DARPP-32 phosphorylation at threonine 75 in isolated medium spiny neurons.
In a model of subarachnoid hemorrhage, rats with hydrocephalus had more severe basal ganglia injury with greater DARPP-32 depletion than rats without hydrocephalus.
Title: Basal Ganglia Damage in Experimental Subarachnoid Hemorrhage.
we also demonstrated frequent co-overexpression and positive correlation of DARPP-32, SRp20 and CD44E expression levels in human gastric primary tumors.
Title: Regulation of CD44E by DARPP-32-dependent activation of SRp20 splicing factor in gastric tumorigenesis.
Darpp32 interaction with beta-adducin may be the molecular pathway by which environmental changes may rapidly alter responsiveness of striatal neurons involved in the reward system.
Title: DARPP-32 interaction with adducin may mediate rapid environmental effects on striatal neurons.
Study demonstrated that DARPP-32 was not altered in prefrontal cortex and hippocampus in sepsis
Title: Evaluation of NCS-1, DARPP-32, and neurotrophins in hippocampus and prefrontal cortex in rats submitted to sepsis.
In cortical & striatal brain regions, 3 different neuronal populations either expressing SSTRs & DARPP-32 alone or displaying colocalization were identified. SSTR-positive neurons lacking DARPP-32 colocalization may be spared in excitotoxicity.
Title: Colocalization of somatostatin receptors with DARPP-32 in cortex and striatum of rat brain.
DARPP32 has a role in pluripotent stem cell differentiation toward medium-sized spiny neurons
Title: Developmentally coordinated extrinsic signals drive human pluripotent stem cell differentiation toward authentic DARPP-32+ medium-sized spiny neurons.
This study demonistrated that Changes in nucleus accumbens and neostriatal c-Fos and DARPP-32 immunoreactivity during different stages of food-reinforced instrumental training
Title: Changes in nucleus accumbens and neostriatal c-Fos and DARPP-32 immunoreactivity during different stages of food-reinforced instrumental training.

Submit: New GeneRIF Correction See all GeneRIFs (34)

General gene information

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Markers

RH130479 (e-PCR)
- UniSTS:213763

AU040756 (e-PCR)
- UniSTS:160153

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables D1 dopamine receptor binding	IPI Inferred from Physical Interaction more info	PubMed
enables D2 dopamine receptor binding	IPI Inferred from Physical Interaction more info	PubMed
enables D3 dopamine receptor binding	IPI Inferred from Physical Interaction more info	PubMed
enables D4 dopamine receptor binding	IPI Inferred from Physical Interaction more info	PubMed
enables D5 dopamine receptor binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein phosphatase inhibitor activity	IEA Inferred from Electronic Annotation more info
enables protein phosphatase inhibitor activity	ISO Inferred from Sequence Orthology more info
enables protein phosphatase inhibitor activity	TAS Traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within DNA-templated transcription	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within behavioral response to cocaine	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cellular response to cocaine	ISO Inferred from Sequence Orthology more info
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in intracellular signal transduction	IDA Inferred from Direct Assay more info	PubMed
involved_in locomotory behavior	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within locomotory behavior	ISO Inferred from Sequence Orthology more info
involved_in memory	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of female receptivity	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within response to amphetamine	ISO Inferred from Sequence Orthology more info
involved_in response to cocaine	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within response to morphine	ISO Inferred from Sequence Orthology more info
involved_in response to nicotine	IDA Inferred from Direct Assay more info	PubMed
involved_in signal transduction	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within visual learning	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	TAS Traceable Author Statement more info
located_in dendritic spine head	IDA Inferred from Direct Assay more info	PubMed
located_in dendritic spine neck	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in neuronal cell body	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
is_active_in postsynapse	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: protein phosphatase 1 regulatory subunit 1B

Names: dopamine- and cAMP-regulated neuronal phosphoprotein; dopamine- and cAMP-regulated phosphoprotein DARPP-32; neuronal phosphoprotein DARPP-32

NP_612530.1

EC 3.1.3.16

XP_006247406.1

EC 3.1.3.16

XP_063125491.1

EC 3.1.3.16

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_138521.1 → NP_612530.1 protein phosphatase 1 regulatory subunit 1B

See identical proteins and their annotated locations for NP_612530.1

Status: PROVISIONAL

Source sequence(s)

AY601872

UniProtKB/Swiss-Prot

Q6J4I0

UniProtKB/TrEMBL

A0A8I5YCG8

Related

ENSRNOP00000077715.2, ENSRNOT00000107401.2

Conserved Domains (1) summary

pfam05395
Location:2 → 112

DARPP-32; Protein phosphatase inhibitor 1/DARPP-32

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086028.1 Reference GRCr8

Range

83843948..83853063

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_063269421.1 → XP_063125491.1 protein phosphatase 1 regulatory subunit 1B isoform X2

UniProtKB/TrEMBL

A0A8I5YCG8
XM_006247344.5 → XP_006247406.1 protein phosphatase 1 regulatory subunit 1B isoform X1

UniProtKB/TrEMBL

A0A8I6GL91

Related

ENSRNOP00000094600.1, ENSRNOT00000109859.2

Conserved Domains (1) summary

pfam05395
Location:118 → 150

DARPP-32; Protein phosphatase inhibitor 1/DARPP-32