top-2 DNA topoisomerase 2 top-2 [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: top-2
Official Full Name: DNA topoisomerase 2 top-2
Primary source: WormBase:WBGene00010785
Locus tag: CELE_K12D12.1
See related: AllianceGenome:WB:WBGene00010785
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Predicted to enable several functions, including ATP binding activity; DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity; and metal ion binding activity. Involved in mitotic sister chromatid separation. Predicted to be located in several cellular components, including chromosome; nucleoplasm; and spindle. Predicted to be active in nucleus. Is expressed in embryonic cell; germ cell; germ line; and somatic cell. Orthologous to several human genes including TOP2B (DNA topoisomerase II beta). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See top-2 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 7

Sequence:: Chromosome: II; NC_003280.10 (11875148..11880588)

Chromosome II - NC_003280.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003280.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

A global chromatin compaction pathway that represses germline gene expression during starvation.
Title: A global chromatin compaction pathway that represses germline gene expression during starvation.
Topo-II promotes chromosome-length shortening.
Title: CENP-A and topoisomerase-II antagonistically affect chromosome length.
These results explain why hTOPIIalpha and hTOPIIbeta are differentially affected by various poisons and demonstrate the utility of C. elegans in understanding the genetics of drug responses.
Title: Natural variation in a single amino acid substitution underlies physiological responses to topoisomerase II poisons.
Novel Top-2 mutation disrupts chromosome segregation during spermatogenesis.
Title: The Identification of a Novel Mutant Allele of topoisomerase II in Caenorhabditis elegans Reveals a Unique Role in Chromosome Segregation During Spermatogenesis.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

ZK1127.7 (e-PCR)
- UniSTS:310419

K12D12.1 (e-PCR)
- UniSTS:306363

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA topoisomerase activity	IEA Inferred from Electronic Annotation more info
enables DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA metabolic process	IEA Inferred from Electronic Annotation more info
involved_in DNA topological change	IEA Inferred from Electronic Annotation more info
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in meiotic cell cycle	IEA Inferred from Electronic Annotation more info
involved_in mitotic sister chromatid segregation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mitotic sister chromatid separation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in resolution of meiotic recombination intermediates	IBA Inferred from Biological aspect of Ancestor more info
involved_in sister chromatid segregation	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in spindle	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: DNA topoisomerase 2 top-2

NP_496536.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003280.10 Reference assembly

Range

11875148..11880588

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_064135.5 → NP_496536.1 DNA topoisomerase 2 top-2 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_496536.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q23670, Q27509

Conserved Domains (7) summary

cd03365
Location:490 → 610

TOPRIM_TopoIIA; TOPRIM_TopoIIA: topoisomerase-primase (TOPRIM) nucleotidyl transferase/hydrolase domain of the type found in proteins of the type IIA family of DNA topoisomerases similar to Saccharomyces cerevisiae Topoisomerase II. TopoIIA enzymes cut both strands of ...

cd00187
Location:746 → 1222

TOP4c; DNA Topoisomerase, subtype IIA; domain A'; bacterial DNA topoisomerase IV (C subunit, ParC), bacterial DNA gyrases (A subunit, GyrA),mammalian DNA toposiomerases II. DNA topoisomerases are essential enzymes that regulate the conformational changes in DNA ...

cd03481
Location:300 → 452

TopoIIA_Trans_ScTopoIIA; TopoIIA_Trans_ScTopoIIA: Transducer domain, having a ribosomal S5 domain 2-like fold, of the type found in proteins of the type IIA family of DNA topoisomerases similar to Saccharomyces cerevisiae Topo IIA. S. cerevisiae Topo IIA is a homodimer encoded ...

PTZ00108
Location:58 → 1520

PTZ00108; DNA topoisomerase 2-like protein; Provisional

pfam02518
Location:115 → 212

HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase

pfam08070
Location:1428 → 1513

DTHCT; DTHCT (NUC029) region

pfam16898
Location:608 → 746

TOPRIM_C; C-terminal associated domain of TOPRIM