lin-5 Spindle apparatus protein lin-5 [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: lin-5
Official Full Name: Spindle apparatus protein lin-5
Primary source: WormBase:WBGene00002994
Locus tag: CELE_T09A5.10
See related: AllianceGenome:WB:WBGene00002994
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables dynein complex binding activity. Involved in several processes, including gonad development; microtubule cytoskeleton organization; and regulation of protein localization. Located in cell cortex; condensed chromosome; and spindle. [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See lin-5 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 6

Sequence:: Chromosome: II; NC_003280.10 (7860574..7863563)

Chromosome II - NC_003280.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003280.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

mitotic kinase PLK-1 affects cell polarity via the enrichment in somatic AB cells, which limits the cortical localization of the dynein-binding NuMA orthologue LIN-5
Title: Cell polarity-dependent centrosome separation in the C. elegans embryo.
LIN-5 is required for EMS spindle positioning, possibly through a Galpha- and DLG-1-independent mechanism.
Title: Mitotic Spindle Positioning in the EMS Cell of Caenorhabditis elegans Requires LET-99 and LIN-5/NuMA.
Four distinct in vivo phosphorylated LIN-5 residues were found to have critical functions in spindle positioning. Two of these residues form part of a 30 amino acid binding site for GPR-1, which we identified by reverse two-hybrid screening. These LIN-5 phosphorylations promote LIN-5-GPR-1/2 interaction and contribute to cortical pulling forces
Title: Multisite Phosphorylation of NuMA-Related LIN-5 Controls Mitotic Spindle Positioning in C. elegans.
Data show that Phospho-LIN-5 co-localizes with PKC-3 at the anterior cell cortex and temporally coincides with a switch from anterior- to posterior-directed spindle movements in the one-cell embryo.
Title: aPKC phosphorylates NuMA-related LIN-5 to position the mitotic spindle during asymmetric division.
ASPM-1, together with calmodulin (CMD-1), promotes meiotic spindle organization and the accumulation of LIN-5 at meiotic and mitotic spindle poles.
Title: NuMA-related LIN-5, ASPM-1, calmodulin and dynein promote meiotic spindle rotation independently of cortical LIN-5/GPR/Galpha.
Dynamic localization of lin-5 to cell cortex forces generation of domains during spindle positioning.
Title: Dynamic localization of LIN-5 and GPR-1/2 to cortical force generation domains during spindle positioning.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

T09A5.10 (e-PCR)
- UniSTS:307978

T09A5.9 (e-PCR)
- UniSTS:307983

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables dynein complex binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in cell cycle	IEA Inferred from Electronic Annotation more info
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in establishment of meiotic spindle orientation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in establishment of meiotic spindle orientation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of mitotic spindle localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in gonad development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in locomotion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic spindle elongation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nervous system development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of protein localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in reproductive process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cell cortex	IDA Inferred from Direct Assay more info	PubMed
located_in cell cortex	IEA Inferred from Electronic Annotation more info
located_in centrosome	IEA Inferred from Electronic Annotation more info
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in chromosome, centromeric region	IEA Inferred from Electronic Annotation more info
located_in condensed chromosome	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in kinetochore	IEA Inferred from Electronic Annotation more info
located_in kinetochore microtubule	IDA Inferred from Direct Assay more info	PubMed
is_active_in microtubule organizing center	IBA Inferred from Biological aspect of Ancestor more info
located_in spindle	IDA Inferred from Direct Assay more info	PubMed
located_in spindle	IEA Inferred from Electronic Annotation more info
located_in spindle midzone	IDA Inferred from Direct Assay more info	PubMed
located_in spindle pole	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Spindle apparatus protein lin-5

NP_495654.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003280.10 Reference assembly

Range

7860574..7863563

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_063253.9 → NP_495654.1 Spindle apparatus protein lin-5 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_495654.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P45970

Conserved Domains (3) summary

COG4985
Location:411 → 491

COG4985; ABC-type phosphate transport system, auxiliary component [Inorganic ion transport and metabolism]

pfam15035
Location:217 → 392

Rootletin; Ciliary rootlet component, centrosome cohesion

pfam15905
Location:161 → 526

HMMR_N; Hyaluronan mediated motility receptor N-terminal