mdf-1 uncharacterized protein [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: mdf-1
Official Full Name: uncharacterized protein
Primary source: WormBase:WBGene00003160
Locus tag: CELE_C50F4.11
See related: AllianceGenome:WB:WBGene00003160
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables protein kinase binding activity. Involved in embryo development; mitotic spindle assembly; and regulation of mitotic sister chromatid separation. Located in kinetochore; nuclear membrane; and nuclear periphery. Is expressed in several structures, including Z2; Z3; head neurons; intestine; and somatic gonad precursor. Human ortholog(s) of this gene implicated in lymphoma; mosaic variegated aneuploidy syndrome 7 with inflammation and tumor predisposition; and prostate cancer. Orthologous to human MAD1L1 (mitotic arrest deficient 1 like 1). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See mdf-1 in Genome Data Viewer

Location:: chromosome: V

Exon count:: 5

Sequence:: Chromosome: V; NC_003283.11 (9541782..9544226, complement)

Chromosome V - NC_003283.11 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003283.11

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Mad1's ability to interact with Mad2 is essential to regulate and monitor meiotic synapsis in C. elegans.
Title: Mad1's ability to interact with Mad2 is essential to regulate and monitor meiotic synapsis in C. elegans.
depletion of either MAD1 or CENPA results in loss of peripherally associated damaged DNA.
Title: DNA damage response and spindle assembly checkpoint function throughout the cell cycle to ensure genomic integrity.
spindle assembly checkpoint components MAD-1, MAD-2, and BUB-3 are required to negatively regulate synapsis and promote the synapsis checkpoint response.
Title: Spindle assembly checkpoint proteins regulate and monitor meiotic synapsis in C. elegans.
126 of the possible 954 predicted monoG/C tracts, larger than 14 bp, were deleted in mdf-1.
Title: Spectrum of variations in dog-1/FANCJ and mdf-1/MAD1 defective Caenorhabditis elegans strains after long-term propagation.
NPP-5 interacts genetically and physically with SAC protein MAD1/MDF-1, whose nuclear envelope accumulation requires NPP-5.
Title: Dissection of the NUP107 nuclear pore subcomplex reveals a novel interaction with spindle assembly checkpoint protein MAD1 in Caenorhabditis elegans.
Data show that doubling the CYB-3 (Cyclin B3) dosage suppresses sterility in the absence of the essential spindle assembly checkpoint component MDF-1 without causing a delay in the onset of anaphase.
Title: Duplication of cyb-3 (cyclin B3) suppresses sterility in the absence of mdf-1/MAD1 spindle assembly checkpoint component in Caenorhabditis elegans.
These results suggest that SPDL-1 functions in a kinetochore receptor of MDF-1/MAD1 to induce spindle assembly checkpoint function.
Title: SPDL-1 functions as a kinetochore receptor for MDF-1 in Caenorhabditis elegans.
MDF-1 causes the primordial germ cells arrest by two mechanisms, one involving MDF-2 and another that is independent of other spindle assembly checkpoint components.
Title: Spindle assembly checkpoint gene mdf-1 regulates germ cell proliferation in response to nutrition signals in C. elegans.
Deletion of the spindle checkpoint gene mdf-1 blocks lissencephaly disease gene lis-1-induced cell cycle arrest and germline apoptosis, placing the spindle checkpoint pathway upstream of the programmed cell death pathway.
Title: Progression from mitotic catastrophe to germ cell death in Caenorhabditis elegans lis-1 mutants requires the spindle checkpoint.
describe 11 suppressors of the mdf-1(gk2) lethality, 10 identified in an ethyl methanesulfonate (EMS) mutagenesis screen and 1 isolated using the dog-1(gk10) (deletions of guanine-rich DNA) mutator strain
Title: Suppressors of spindle checkpoint defect (such) mutants identify new mdf-1/MAD1 interactors in Caenorhabditis elegans.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

STS5-179 (e-PCR)
- UniSTS:242338

C50F4.11 (e-PCR)
- UniSTS:307365

C50F4.5 (e-PCR)
- UniSTS:320698

C50F4.13 (e-PCR)
- UniSTS:320487

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in attachment of mitotic spindle microtubules to kinetochore	IBA Inferred from Biological aspect of Ancestor more info
involved_in embryo development ending in birth or egg hatching	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic spindle assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic spindle assembly checkpoint signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic spindle assembly checkpoint signaling	IEA Inferred from Electronic Annotation more info
involved_in regulation of mitotic sister chromatid separation	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in intracellular organelle	IEA Inferred from Electronic Annotation more info
is_active_in kinetochore	IBA Inferred from Biological aspect of Ancestor more info
located_in kinetochore	IDA Inferred from Direct Assay more info	PubMed
is_active_in mitotic spindle	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nuclear envelope	IBA Inferred from Biological aspect of Ancestor more info
located_in nuclear membrane	IMP Inferred from Mutant Phenotype more info	PubMed
located_in nuclear periphery	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: uncharacterized protein

NP_505462.1

Product from WormBase gene class mdf;
Confirmed by transcript evidence; MAD (yeast Mitosis arrest DeFicient) related

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003283.11 Reference assembly

Range

9541782..9544226 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_073061.5 → NP_505462.1 uncharacterized protein CELE_C50F4.11 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_505462.1

Status: REVIEWED

UniProtKB/TrEMBL

G5EFM0

Conserved Domains (2) summary

TIGR02168
Location:284 → 471

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

pfam05557
Location:392 → 656

MAD; Mitotic checkpoint protein